Potential Prognostic Significance of Cytometrically Determined DNA Abnormality in GI Tract Human Tumors<sup>a</sup>

Teodori, Laura; Tirindelli-Danesi, Donatella; Cordelli, Eugenia; Uccelli, Raffaella; Vita, R. De; Spanò, Marcello; Mauro, F.; Schillaci, Alberto; Moraldi, A; Capurso, Lucio; Stipa, S

doi:10.1111/j.1749-6632.1986.tb42047.x

Cited by 11 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2) suggests that the DI value increases from benign to malignant lesions. These data are in agreement with previous preliminary observations obtained with colorectal adenomas and adenocarcinomas (7,20) and with chronic atrophic gastritis and gastric carcinomas (18). DNA aneuploidy incidence and DNA index values also seem to increase for the adenomas with foci of adenocarcinomas, a group of special interest since they represent a link in the colorectal adenoma-adenocarcinoma sequence.…”

Section: Discussionsupporting

confidence: 82%

DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas

Giaretti¹,

Sciallero

Bruno³

et al. 1988

Cytometry

View full text Add to dashboard Cite

DNA ploidy of 64 colorectal adenomas and 49 adenocarcinomas, examined endoscopically, was studied by flow cytometry. We found DNA aneuploidy in none of the 105 normal mucosa samples (O%), in 20 adenomas (31%), and in 36 adenocarcinomas (74%). DNA ploidy of adenomas correlated with size ( P = 0.02) and degree of dysplasia ( P < 0.01) but not with histologic type. Adenomas had a 45% incidence of DNA aneuploid stem lines in the DNA index range of 0.80-1.20, compared with 8% in the case of adenocarcinomas. The distribution of the DNA index values of adenocarcinomas was approximately normal, with a mean value 1.63 f 0.28. The mean DNA index for the three cases of "carcinoma in adenoma" with invasion of the stalk of the adenoma was 1.52 f 0.18. These results, using DNA flow cytometry, provide evidence for the progression of colorectal adenoma to adenocarcinoma. The classification of adenomas according to DNA ploidy may be information of considerable practical value to the clinician in predicting risk of further adenomas andlor risk of cancer.

show abstract

Section: Discussionsupporting

confidence: 82%

DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas

Giaretti¹,

Sciallero

Bruno³

et al. 1988

Cytometry

View full text Add to dashboard Cite

show abstract

“…A large amount of studies are dealing with the cellular DNA content (20,21). However, the reported controversial results, in our opinion, are also due to the monitoring of one parameter-DNA content alone-that is unable to discriminate abnormal diploid from normal diploid clones or small aneuploid clones, thus underestimating the presence of aneuploidy.…”

mentioning

confidence: 94%

DNA/protein flow cytometry as a predictive marker of malignancy in dysplasia-free Barrett's esophagus: Thirteen-year follow-up study on a cohort of patients

et al. 1998

View full text Add to dashboard Cite

Intestinal metaplasia identifies Barrett's esophagus (BE) and is associated with an increased risk for esophageal adenocarcinoma. Dysplasia occurs as an intermediate step. However, progression from metaplasia to neoplasia without the demonstration of dysplasia has been described. The role of dual‐parameter flow cytometry (FC) as a predictor of neoplastic risk in dysplasia‐free cases was evaluated. DNA/protein FC and histology were performed on 362 samples from 30 dysplasia‐free BE patients, followed up since 1985 once every 1–2 years. Nine cases were aneuploid, five of which (group IV) were frankly aneuploid; in the other four cases (group III), aneuploidy was detectable by dual‐parameter analysis only. Twenty‐one patients were diploid. Twelve (group II) also had an abnormally high G1‐phase protein content compared to group I (nine patients), which were diploid with a low‐moderate protein content. In three patients of group IV an adenocarcinoma in situ was diagnosed, after 5, 6, and 10 years, respectively. In two patients of group III, a low‐ and a high‐grade dysplasia were observed at 3 and 6 years follow‐up, respectively. One patient of group I first acquired a high protein content, then an aneuploid DNA content, and then progressed to adenocarcinoma (12 years). None of the still diploid patients (17 cases) have progressed to dysplasia or cancer compared with 6 of 13 presently aneuploid patients (P < 0.01). In conclusion, DNA/protein FC is a marker of increased malignant potential and thus may be used to detect patients at higher risk in dysplasia‐free BE and assist in understanding the various stages of malignant transformation in long‐term follow‐up studies. Cytometry (Comm. Clin. Cytometry) 34:257–263, 1998. © 1998 Wiley‐Liss, Inc.

show abstract

Flow cytometric analysis of nuclear DNA content in esophageal cancer. Aneuploidy as an index for highly malignant potential

Kaketani

Saito

Kobayashi

1989

Cancer

View full text Add to dashboard Cite

show abstract

Potential Prognostic Significance of Cytometrically Determined DNA Abnormality in GI Tract Human Tumors^a

Cited by 11 publications

References 6 publications

DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas

DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas

DNA/protein flow cytometry as a predictive marker of malignancy in dysplasia-free Barrett's esophagus: Thirteen-year follow-up study on a cohort of patients

Flow cytometric analysis of nuclear DNA content in esophageal cancer. Aneuploidy as an index for highly malignant potential

Contact Info

Product

Resources

About

Potential Prognostic Significance of Cytometrically Determined DNA Abnormality in GI Tract Human Tumorsa

Cited by 11 publications

References 6 publications

DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas

DNA flow cytometry of endoscopically examined colorectal adenomas and adenocarcinomas

DNA/protein flow cytometry as a predictive marker of malignancy in dysplasia-free Barrett's esophagus: Thirteen-year follow-up study on a cohort of patients

Flow cytometric analysis of nuclear DNA content in esophageal cancer. Aneuploidy as an index for highly malignant potential

Contact Info

Product

Resources

About

Potential Prognostic Significance of Cytometrically Determined DNA Abnormality in GI Tract Human Tumors^a