Potential Psyllid Vectors of Candidatus Phytoplasma Mali and Candidatus Phytoplasma Pyri in Turkey

Kaya, Kamuran; Serçe, Çiğdem Ulubaş; Gazel, Mona; Çağlayan, Kadriye; Sauvion, Nicolas

doi:10.21162/pakjas/16.3804

Cited by 7 publications

(3 citation statements)

References 37 publications

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“…Dysaphis plantaginea is not known as a vector of plant viruses, contrary to C. pyri, which transmits phytoplasma. The use of garlic EOs could limit pathogen transmission by C. pyri as it is linked to phloem activity (Mustafa et al, 2015;Kaya et al, 2016), which was negatively impacted in this study. Furthermore, notably in aphids, a decrease in phloem sap ingestion is often linked to a decrease in survival and/or fecundity and, consequently, a decrease in vector population (Chesnais et al, 2019).…”

Section: Discussionmentioning

confidence: 84%

Behavioral disruption of two orchard hemipteran pests by garlic essential oil

Denoirjean

Rivière

Doury

et al. 2022

Entomologia Exp Applicata

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Manipulating the odorscape of insects is a key component of most pest control strategies. It can be achieved using natural chemical products such as those emitted by nonhost plants (Conchou et al., 2019). In particular, companion plants can disrupt the pest olfactory environment, owing to the chemical masking properties and/or repelling effects of non-host volatile organic compounds (VOCs) they naturally emit when associated with focal crops (Parker et al., 2013). Exploiting other sources of non-host plantderived odors can be attained with essential oils (EOs) that provide a wide range of bioactive compounds. Distilled from plants, EOs are highly volatile secondary metabolites that may play a key role in plant-insect interactions, such as alkaloids, amides, chalcones, flavones, kawapirones, lignans, neolignans, or phenols (

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Section: Discussionmentioning

confidence: 84%

Behavioral disruption of two orchard hemipteran pests by garlic essential oil

Denoirjean

Rivière

Doury

et al. 2022

Entomologia Exp Applicata

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“…It is currently unclear how specific the relationships between a given Liberibacter species and psyllid vector are, but some species can have more than one psyllid vector (Haapalainen, 2014). In addition to being the primary and usually exclusive vectors of Ca.Liberibacter, psyllids are also common vectors of phytoplasmas, another group of mycoplasma-like bacterial plant pathogens (Ethmayer et al , 2011;Jarausch et al , 2019;Kaya et al , 2016).…”

Section: Introductionmentioning

confidence: 99%

Detection and identification of a Candidatus Liberibacter species from ash tree infesting psyllids

Wamonje¹,

Zhou²,

Bamrah³

et al. 2020

Preprint

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Candidatus Liberibacter species cause severe, economically important diseases. All known species of these pathogens are putatively insect-transmitted, specifically by psyllids. Detection of Liberibacter in plants is complicated by its uneven distribution in host plants and largely unculturable nature. The death of black (Fraxinus nigra) and mancana (Fraxinus mandshurica) ash trees in Saskatchewan, Canada has been associated with infestation with the cottony ash psyllid (Psyllopsis discrepans). We hypothesized that the symptoms and death could be due to a psyllid-transmitted Liberibacter. We used a combination of conventional PCR and Sanger sequencing of the 16S rDNA for detection of Liberibacter, and the genes CO1 and Cyt-b to determine species of psyllids. The 16S sequencing generated two sequences, NTHA 5 (GenBank accession number MK942379) and NTHA 6 (GenBank accession number MK937570) that were 1058 and 1085 bp long. A BLAST search for homology showed 99-100% sequence similarity to a Candidatus Liberibacter solanacearum sequence (GenBank accession number: KX197200) isolated from the Nearctic psyllid (Bactericera maculipennis) of US provenance. CO1 and Cyt-b gene sequencing of our psyllids yielded sequence information confirming that they were P. discrepans from comparisons with sequences in GenBank and BOLD. Confirmatory sequence comparison with a reference sample from the United Kingdom was concurrent. These results provide the first evidence on the likely cause of ash dieback in Saskatchewan. Further, they suggest a relatively rare example of a Liberibacter adapting a new host plant.

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“…Phytoplasma pyri' and 'Ca. Phytoplasma mali' (Sertkaya et al 2005;Ulubaş Serçe et al 2006;Canik & Ertunç 2007;Gazel et al 2007;Sertkaya et al 2008a, b;Kaya et al 2016;Canik Orel et al 2019).…”

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confidence: 99%

First report of 'Candidatus Phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis L.)

Usta¹,

Güller²,

Sipahioğlu³

2021

Plant Prot. Sci.

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The natural occurrence of 'Candidatus Phytoplasma trifolii' in pear trees (Pyrus communis Linnaeus) is reported here for the first time. In 2017, a total of thirty-five pear trees, two of them exhibiting leaf rolling along the midvein, reddening, bushy appearance, and upright growth symptoms were sampled in different locations in Van province, Turkey. The total deoxyribonucleic acid was extracted from symptomatic and asymptomatic plants. The purified DNA served as a template in nested polymerase chain reaction (nested-PCR) assays, performed to amplify 16S rRNA sequences using universal primer pairs (R16mF2/R16mR1 and R16F2n/R16R2). The resulting PCR products were then cloned into a pGEM T-Easy vector and sequenced bidirectionally. The phytoplasma strain, group, and subgroup identity were determined using the in silico restriction fragment length polymorphism (RFLP) analysis of the 16S ribosomal RNA-encoding gene sequences profiling with seventeen distinct restriction enzymes. Of the thirty-five pear samples, only two yielded 1 256 bp and 1 258 bp DNA fragments and were designated as Van-Pr3 (Acc. No. MH709141) and Van-Pr4 (Acc. No. MH730561), respectively. Based on the in silico virtual RFLP pattern analysis of the 16S rRNA sequences, we confirmed the presence of 'Ca. P. trifolii' belonging to the clover proliferation group and both identified phytoplasmas were identical with the similarity coefficient of 1.00 to the reference pattern of 16Sr group VI, subgroup A (Acc. No. AY390261). Here we report that the pear tree is an alternate host of the 'Ca. P. trifolii'.

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Potential Psyllid Vectors of Candidatus Phytoplasma Mali and Candidatus Phytoplasma Pyri in Turkey

Cited by 7 publications

References 37 publications

Behavioral disruption of two orchard hemipteran pests by garlic essential oil

Behavioral disruption of two orchard hemipteran pests by garlic essential oil

Detection and identification of a Candidatus Liberibacter species from ash tree infesting psyllids

First report of 'Candidatus Phytoplasma trifolii' associated with leaf reddening and upright growth in pears (Pyrus communis L.)

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