Potential‐Responsive Surfaces for Manipulation of Cell Adhesion, Release, and Differentiation

Abstract

In living systems, interfacial molecular interactions control many biological processes. New stimuli‐responsive strategies are desired to provide versatile model systems that can regulate cell behavior in vitro. Described here are potential‐responsive surfaces that control cell adhesion and release as well as stem cell differentiation. Cell adhesion can be modulated dynamically by applying negative and positive potentials to surfaces functionalized with tailored monolayers. This process alters cell morphology and ultimately controls behavior and the fate of the cells. Cells can be detached from the electrode surface as intact clusters with different geometries using electrochemical potentials. Importantly, morphological changes during adhesion guide stem cell differentiation. The higher accessibility of the peptide under a positive applied potential causes phenotypic changes in the cells that are hallmarks of osteogenesis, whereas lower accessibility of the peptide promoted by negative potentials leads to adipogenesis.

Peptide-Functionalized Nanostructured Microarchitectures Enable Rapid Mechanotransductive Differentiation

“…Electrodeposition was performed through direct current potential amperometry to grow nanostructured gold on the apertures. ,, In this study, a high degree of nanostructuring was introduced as it has been shown previously to more efficiently upregulate neural differentiation . Following electrodeposition, a self-assembled monolayer (SAM) was formed on the surface of NMs by depositing 11-mercaptoundecanoic acid (MUA) . The carboxylic group of the MUA was then conjugated with the RGD peptide through 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/ N -hydroxysuccinimide (EDC/NHS) chemistry to form RGD-functionalized NMs.…”

Directed Conformational Switching of a Zinc Finger Analogue Regulates the Mechanosensing and Differentiation of Stem Cells

“…A number of fascinating strategies to temporally or reversibly manipulate the presentation of bio-ligands using various external stimuli have been proposed to tune cellmaterial interactions, such as light, [5] electric fields, [6] supramolecular interactions, [7] and magnetic fields. [8] For instance, light [5e] and electrical potential [6] decrease or increase the RGD accessibility to the cells, thereby, suppressing or facilitating cell adhesion on materials, respectively. Sugar-responsive catechol-boronate chemistry and fluidic surface coatings via supramolecular assembly can dynamically present the cell-binding motifs to modulate cell adhesion.…”

MSN based biointerfaces to advance knowledge on ligand-stem cell interaction

“…The release of Azo-E7 peptides weakened adhesion and mobility of BMSCs [131] Electric potential RGD/Sulfonate/Au Switchable surfaces dynamically modulated stem cell adhesion, morphology and differentiation, with a higher RGD accessibility leading to osteogenesis [132] Ligand mobility Magnetic force PEG-MNP@SiO2-RGD Low RGD mobility promoted adhesion, spreading, osteogenic differentiation of hMSCs [133] Magnetic force PEG-SPION@SiO2-RGD Nanoscale motion of RGD influenced the adhesion and differentiation of hMSCs both in vitro and in vivo [134] 4…”

“…In another example of electrochemically responsive platforms, a mixture of RGDmodified 11-mercaptoundecanoic acid (MUA) and negatively charged, sulfonate moieties-modified MUA was used to modify a gold surface to form an electrodepatterned, self-assembled monolayer (SAM) [132]. When applying a negative voltage, the negatively charged sulfonate moieties were repelled from the gold electrode surface, which concealed RGD.…”