Potential risk of monochorionic dizygotic twin blastocyst formation associated with early laser zona dissection of group cultured embryos

Schiewe, M.C.; Whitney, John P.; Anderson, Robert E.

doi:10.1016/j.fertnstert.2014.11.009

Cited by 18 publications

(25 citation statements)

References 31 publications

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“…Day 5 and Day 6 blastocyst ICM and TE were independently graded from A to C quality, with A=excellent, B=good-fair and C=poor [1]. All blastocysts minimally achieved full blastocoele expansion prior to grading [8]. Grading was recorded for ICM first followed by TE.…”

Section: Methodsmentioning

confidence: 99%

“…Using MCO-5M mini Sanyo/Panasonic tri-gas incubators (5% 02/5.3-6.0% CO 2 ), we group cultured up to 8 embryos per 25 uL droplet of Global™ medium (LG; Life Global, Guilford, CT) supplemented with 7.5% synthetic protein supplement under Ovoil™ (Vitrolife, Englewood, CO) until blastocyst biopsy [8]. All oocytes retrieved were evaluated for maturity and had intracytoplasmic sperm injection (ICSI) performed 3-6 hours post egg retrieval [9].…”

Section: Embryo Culture Biopsy and Pgs Testingmentioning

confidence: 99%

“…All oocytes retrieved were evaluated for maturity and had intracytoplasmic sperm injection (ICSI) performed 3-6 hours post egg retrieval [9]. Embryos were initially evaluated on day 3; laser zona dissection was performed using a 1410-nm diode laser (Zilos-tk™; Hamilton Thorne, Beverly, MA) and embryo incubation continued until day 5/6 evaluations [8]. The zona opening created on day 3 allowed TE to prematurely rupture through a 10-12-µm furrow in the zona.…”

Section: Embryo Culture Biopsy and Pgs Testingmentioning

confidence: 99%

“…The zona opening created on day 3 allowed TE to prematurely rupture through a 10-12-µm furrow in the zona. The same laser was used on day 5/6 for biopsying using a combination of laser pulses and mechanical separation to achieve 3-10 TE cells [4,8] used for aCGH and NextGen PGS (Genesis Genetics, Plymouth, MI).…”

Section: Embryo Culture Biopsy and Pgs Testingmentioning

confidence: 99%

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Euploidy Predictability of Human Blastocyst Inner Cell Mass and Trophectoderm Grading

Whitney¹,

Anderson²

2015

Ann Clin Lab Res

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Background: The selection of viable, potentially normal, embryos is vital to successful embryo transfers. Subjective morphology grading of blastocysts has been the most accepted and reliable standard to judge embryo quality. However, good to excellent embryo development alone does not necessarily insure euploidy. Our objective was to determine if independent quality grades for blastocyst inner cell mass (ICM) and trophectoderm (TE) can predict early embryo euploidy. Secondly, we aimed to develop a comparison chart for preference of blastocyst quality grades utilizing both ICM and TE grades to improve the decision making process.

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Section: Methodsmentioning

confidence: 99%

Section: Embryo Culture Biopsy and Pgs Testingmentioning

confidence: 99%

Section: Embryo Culture Biopsy and Pgs Testingmentioning

confidence: 99%

Section: Embryo Culture Biopsy and Pgs Testingmentioning

confidence: 99%

See 2 more Smart Citations

Euploidy Predictability of Human Blastocyst Inner Cell Mass and Trophectoderm Grading

Whitney¹,

Anderson²

2015

Ann Clin Lab Res

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“…Meanwhile, the full impact of today's PGS capabilities would be impossible without the advent of vitrification as a highly efficient cryopreservation procedure. Together, the transfer of vitrified-warmed single human euploid blastocysts is capable of achieving >70% implantation rates, independent of age [29].…”

Section: Genetic Testing Of Preimplantation Embryosmentioning

confidence: 99%

The Historic Development and Incorporation of Four Assisted Reproductive Technologies Shaping Today′s IVF Industry

Schiewe¹

2016

JFIV Reprod Med Genet

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The concept of assisted reproductive technologies was most notably derived from the late 19th Century experiments of Sir Walter Heape who successfully transplanted rabbit embryos. Interestingly, it was not until the late 1940's and 1950's that renewed interests in rabbit embryo transfer and cryobiology occurred. The history behind developing effective procedures can be fascinating, though few could be more accidental than Dr. Chris Polge's discovery of glycerol (1948), from a mislabeled bottle of sugar solutions, being an effective cryoprotective agent for sperm freezing. The purpose of this review paper is to discuss four key scientific breakthrough technologies occurring between 1985 and 1995, which ultimately shaped the future of today's human in vitro fertilization (IVF) industry. More importantly, this paper highlights the foundation of underlying related discoveries and some unique stories involving their development and publication. In the end, this paper emphasizes the value of understanding scientific discovery timetables and the eventual re-discovery in the hands and minds of creative, determined and dedicated scientists, as history tends to repeat itself before its useful application is realized.

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Use of imaging software for assessment of the associations among zona pellucida thickness variation, assisted hatching, and implantation of day 3 embryos

Lewis

Farhadifar

Farland

et al. 2017

J Assist Reprod Genet

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show abstract

Potential risk of monochorionic dizygotic twin blastocyst formation associated with early laser zona dissection of group cultured embryos

Cited by 18 publications

References 31 publications

Euploidy Predictability of Human Blastocyst Inner Cell Mass and Trophectoderm Grading

Euploidy Predictability of Human Blastocyst Inner Cell Mass and Trophectoderm Grading

The Historic Development and Incorporation of Four Assisted Reproductive Technologies Shaping Today′s IVF Industry

Use of imaging software for assessment of the associations among zona pellucida thickness variation, assisted hatching, and implantation of day 3 embryos

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