Potential risks of antibiotic resistant bacteria and genes in bioremediation of petroleum hydrocarbon contaminated soils

Cunningham, Colm; Kuyukina, Maria S.; Ившина, И. Б.; Konev, Alexandr I.; Peshkur, Tatyana; Knapp, Charles W.

doi:10.1039/c9em00606k

Cited by 32 publications

(14 citation statements)

References 100 publications

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“…In our studied UWTPs, sul1 bacterial hosts belong to 63 genera (Figure b). Among which, 36 genera (57.14%) were reported as sulfonamide-resistant bacteria through a culture-based method or correlation analysis, − suggesting that the detected hosts by epicPCR were consistent with traditional culture-based methods. Meanwhile, the sul1 gene found in cultured species was most closely related to class 1 integrons, conjugative plasmids, or other mobile genetic elements (MGEs), indicating that this gene was aggressive to transfer among bacterial hosts.…”

Section: Discussionmentioning

confidence: 80%

High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants

Wei

Feng

Yang

et al. 2021

Environ. Sci. Technol.

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The spread of antibiotic resistance genes (ARGs) has gained much attention worldwide, while the contribution of vertical gene transfer (VGT) and horizontal gene transfer (HGT) is still elusive. Here, we improved an emerging high-throughput single-cell-based technology, emulsion, paired isolation, and concatenation polymerase chain reaction (epicPCR), by lengthening the sequence of ARG in the fused ARG-16S rRNA fragments to cover the variance of both ARG and its hosts. The improved epicPCR was applied to track the hosts of a widely detected ARG, sul1 gene, in five urban wastewater treatment plants (UWTPs) during two seasons. The sul1 host bacteria were highly diverse and mostly classified as Proteobacteria and Bacteroidetes. Clear seasonal divergence of α-diversity and interaction networks were present in the host community. The consensus phylogenetic trees of the sul1 gene and their host demonstrated incorrespondence on the whole and regularity on abundant groups, suggesting the important role of both HGT and VGT, respectively. The relative importance of these two ways was further measured; HGT (54%) generally played an equal or even more important role as VGT (46%) in UWTPs. The application of the improved epicPCR technology provides a feasible approach to quantify the relative contributions of VGT and HGT in environmental dissemination of ARGs.

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Section: Discussionmentioning

confidence: 80%

High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants

Wei

Feng

Yang

et al. 2021

Environ. Sci. Technol.

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“…Bioremediation has generally been considered a sustainable approach to managing petroleum hydrocarbon contaminated soils. There has been an increasing focus on 'green' and 'sustainable' remediation and an international standard ISO18504:2017 Soil quality -Sustainable remediation was recently published (2017) [82].…”

Section: Discussionmentioning

confidence: 99%

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Miral

Kautsky

Alves-Carvalho

et al. 2022

Preprint

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As rock-inhabitants, lichens are exposed to extreme and fluctuating abiotic conditions associated with poor sources of nutriments. These extreme conditions confer to lichens the unique ability to develop protective mechanisms. Consequently, lichen-associated microbes disclose highly versatile lifestyles and ecological plasticity, enabling them to withstand extreme environments. Because of their ability to grow on poor and extreme habitats, bacteria associated with lichens can tolerate a wide range of pollutants and on the other hand secrete antimicrobial compounds. In addition, lichen-associated bacteria were described to harbor ecological functions crucial for the evolution of the lichen holobiont. Nevertheless, the ecological features of lichen-associated microbes are still underestimated. To explore the untapped ecological diversity of lichen-associated bacteria, we adopted a novel culturomic approach on the crustose lichen Rhizocarpon geographicum. We sampled R. geographicum in French habitats exposed to oil spill and we combined 9 culturing methods with 16S rRNA sequencing to capture the largest bacterial diversity. A deep functional analysis of the lichen-associated bacterial collection showed the presence of a set of bacterial strains resistant to a wide range of antibiotics and displaying tolerance to Persistent Organic Pollutants (POP). Our study is a starting point to explore the ecological features of the lichen microbiota.

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“…In addition, a list of criteria and properties are needed for establishing a database to help identify and examine the environment for highly pathogenic resistant bacterial types [37][38][39][40][41][42][43][44][45][46][47][48]. Additional procedures that avoid and prevent bacterial antibiotic resistance include the following: regulate the antibiotics given to animals, perform future research that elucidates the mechanisms of antibiotic resistance and the monitoring of resistance [49], improve the laws and regulations to reduce the prevalence of antibiotic resistance, amplify the research of more novel therapies [50], and provide more aid to developing and lowincome countries to more effectively regulate issues of antibiotic resistance [50]. Currently, WHO has an antimicrobial surveillance system that collects data to help reduce the rate of antibiotic resistance [CHokshi].…”

Section: Combating Bacterial Antibiotic Resistancementioning

confidence: 99%

“…AcrS was determined to be an inhibitor of acrD expression. Therefore, inhibiting acrS can export more aminoglycosides through alternative and other RND efflux pumps [49][50][51][52][53][54]. AcrD is a cytoplasmic membrane drug efflux exporter.…”

Section: Rnd-efflux Pumps and Antibiotc-resistancementioning

confidence: 99%

Reducing bacterial antibiotic resistance by targeting bacterial metabolic pathways and disrupting RND efflux pump activity

Hillman¹

2022

Iberoam J Med

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Antibiotic resistance is a significant issue for the medical community, worldwide. Many bacteria develop drug resistance by utilizing multidrug resistant or MDR efflux pumps that can export antibiotics from bacterial cells. Antibiotics are expelled from bacteria by efflux pumps a part of the resistance nodulation division (RND) family. Types of RND efflux pumps include the AcrAB-TolC tripartite protein pump. There are an excessive number of antibiotic compounds that have been discovered; however, only a few antibiotics are effective against MDR bacteria. Many bacteria become drug resistant when sharing genes that encode MDR efflux pump expression. MDR efflux pump encoding genes are incorporated into plasmids and then shared among bacteria. As a consequence, advancements in genetic engineering can sufficiently target and edit pathogenic bacterial genomes for perturbing drug resistance mechanisms. In this perspective and review, support will be provided for utilizing genetic modifications as an antimicrobial approach and tool that may effectively combat bacterial MDR. Ayhan et al. found that deleting acrB, acrA, and tolC increased the levels of antibiotic sensitivity in Escherichia coli. Researchers also found that glucose, glutamate, and fructose all induced the absorption of antibiotics by upregulating the gene expression of maeA and maeB that is a part of the MAL-pyruvate pathway. Therefore, the current perspective and review will discuss the potential efficacy of reducing antibiotic resistance by inhibiting genes that encode efflux protein pump expression while simultaneously upregulating metabolic genes for increased antibiotic uptake.

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Potential risks of antibiotic resistant bacteria and genes in bioremediation of petroleum hydrocarbon contaminated soils

Abstract: The problems associated with potential risks of antibiotic resistance spreading during bioremediation of oil-contaminated soil are discussed. Careful selection of bacterial strains and pretreatment of organic wastes used as fertilizers are suggested.

Cited by 32 publications

References 100 publications

High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants

High-Throughput Single-Cell Technology Reveals the Contribution of Horizontal Gene Transfer to Typical Antibiotic Resistance Gene Dissemination in Wastewater Treatment Plants

Rhizocarpon geographicumlichen discloses a highly diversified microbiota carrying antibiotic resistance and Persistent Organic Pollutants tolerance

Reducing bacterial antibiotic resistance by targeting bacterial metabolic pathways and disrupting RND efflux pump activity

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