Potential Role of Anaerobic Bacteria as Fish Pathogens

Abdelsalam, Mohamed

doi:10.4172/2155-9546.1000500

Cited by 7 publications

(5 citation statements)

References 12 publications

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“…In the current study, NLB samples contained significant proportions of known nitrifying genera ( Mesorhizobium and Burkholderia ), which were not as abundant in LB samples specifically. In turn, Propionibacterium , a gram positive anaerobic taxa group, is commonly associated with skin and gland habitats, where they are able to effectively metabolize carbohydrates and carbon dioxide [ 60 , 61 ]. The most significant change in bacterial community composition in AGD affected gills was the presence of an ASV classified to Tenacibaculum dicentrarchi , which was highly abundant (52.3–85.8%) in 50% of the sampled fish, and strikingly was differentially abundant between LB and NLB groups.…”

Section: Discussionmentioning

confidence: 99%

Bacteriomic Profiling of Branchial Lesions Induced by Neoparamoeba perurans Challenge Reveals Commensal Dysbiosis and an Association with Tenacibaculum dicentrarchi in AGD-Affected Atlantic Salmon (Salmo salar L.)

2020

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Amoebic gill disease is a parasitic condition that commonly affects marine farmed Atlantic salmon. The causative agent, Neoparamoeba perurans, induces a marked proliferation of the gill mucosa and focal superficial necrosis upon branchial lesions. The effect that amoebic branchialitis has upon gill associated commensal bacteria is unknown. A 16S rRNA sequencing approach was employed to profile changes in bacterial community composition, within amoebic gill disease (AGD)-affected and non-affected gill tissue. The bacterial diversity of biopsies with and without diseased tissue was significantly lower in the AGD-affected fish compared to uninfected fish. Furthermore, within the AGD-affected tissue, lesions appeared to contain a significantly higher abundance of the Flavobacterium, Tenacibaculum dicentrarchi compared to adjunct unaffected tissues. Quantitative PCR specific to both N. perurans and T. dicentrarchi was used to further examine the co-abundance of these known fish pathogens. A moderate positive correlation between these pathogens was observed. Taken together, the present study sheds new light on the complex interaction between the host, parasite and bacterial communities during AGD progression. The role that T. dicentrarchi may play in this complex relationship requires further investigation.

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Section: Discussionmentioning

confidence: 99%

Bacteriomic Profiling of Branchial Lesions Induced by Neoparamoeba perurans Challenge Reveals Commensal Dysbiosis and an Association with Tenacibaculum dicentrarchi in AGD-Affected Atlantic Salmon (Salmo salar L.)

2020

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“…However, these findings may also be due to a knowledge gap. Most of the 23 recorded obligatory anaerobes are human, mammal, bird, and/or reptile pathogens (Abdelsalam, 2017). Among the anaerobic bacteria with increased abundance in the present study, Lachnoanaerobaculum, Anaeromassilibacillus, and Youngiibacter can cause disease in humans.…”

Section: Discussionmentioning

confidence: 48%

Metabolomic and metagenomic analyses of the Chinese mitten crab Eriocheir sinensis after challenge with Metschnikowia bicuspidata

et al. 2022

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Milky disease caused by Metschnikowia bicuspidata fungus has significantly harmed the Chinese mitten crab Eriocheir sinensis aquaculture industry. However, the effect of M. bicuspidata infection on the metabolism and intestinal flora of the crab remains unclear. In this study, we aimed to explore the changes in the metabolism and intestinal flora E. sinensis after 48 h of infection with M. bicuspidata, using metabolomic and metagenomic analyses. Metabolomic analysis results revealed 420 significantly different metabolites between the infected and control groups, and these metabolites were enriched in 58 metabolic pathways. M. bicuspidata infection decreased the levels of metabolites related to amino acid biosynthesis, the tricarboxylic acid cycle, as well as lysine, histidine, linolenic, arachidonic, and linoleic acid metabolism. These results indicated that M. bicuspidata infection significantly affected the energy metabolism, growth, and immunity of E. sinensis. The results of metagenomic analysis showed that the anaerobes and ascomycetes populations significantly increased and decreased, respectively, after M. bicuspidata infection. These changes in intestinal flora significantly upregulated metabolic and synthetic pathways while downregulating immunity-related pathways. The results of integrated metabolomic and metagenomic analyses showed that 55 differentially expressed genes and 28 operational taxonomic units were correlated with 420 differential metabolites. Thus, the intestinal flora changes caused by M. bicuspidata infection also affected the metabolites. This study provides novel insights into the metabolic-and intestinal microflora-based effects of M. bicuspidata infection in E. sinensis, as well as a theoretical basis for the interaction between fungi and crustaceans.

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“…are found in the fish gastrointestinal tract ( Wang et al., 2018 ). Abdelsalam (2017) also reported that fish slime, gills and intestines were subjected to contamination with anaerobic bacteria such as C. botulinum, C. perfringens and C. bifermentans, or Actinomyces and Eubacterium tarentallus. Hence, this study pointed out the poor evisceration practices of braised fish vendors and also the inefficiency of the washing operation after evisceration.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of sanitary risks associated with the consumption of street food in the city of Yaoundé (Cameroon): case of braised fish from Mvog-Ada, Ngoa Ekélé, Simbock, Ahala and Olézoa

Maffouo

Mouafo

Mouokeu

et al. 2021

Heliyon

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An investigation was performed to evaluate the braising fish practices and the level of hygiene of the sellers in the city of Yaoundé. Thereafter, five braised carp samples were collected from each of the five selected sites and their microbiological quality assessed. The results showed that all the braising sites do not satisfy the Good Hygiene and Manufacturing Practices. Pathogenic microorganisms such as coliforms (2.17 ± 0.08 to 5.94 ± 0.38 Log cfu/g), E. coli (3.71 ± 0.20 to 6.21 ± 0.48 Log cfu/g) and Salmonella spp. were found in braised carp samples at levels higher than the microbiological criteria of the European Commission which are 1, 1 and 0 Log cfu/g, respectively. Spoilage microorganisms such as yeasts (2.39 ± 0.18 to 3.09 ± 0.23 Log cfu/g) and moulds (2.30 ± 0.10 to 2.92 ± 0.18 Log cfu/g) as well as spore-forming (3.02 ± 0.18 to 4.86 ± 0.35 Log cfu/g) and anaerobic (3.74 ± 0.28 to 4.52 ± 0.38 Log cfu/g) bacteria were also found at higher levels. The study highlights the poor microbiological quality of braised fish sold in the city of Yaoundé.

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Potential Role of Anaerobic Bacteria as Fish Pathogens

Cited by 7 publications

References 12 publications

Bacteriomic Profiling of Branchial Lesions Induced by Neoparamoeba perurans Challenge Reveals Commensal Dysbiosis and an Association with Tenacibaculum dicentrarchi in AGD-Affected Atlantic Salmon (Salmo salar L.)

Bacteriomic Profiling of Branchial Lesions Induced by Neoparamoeba perurans Challenge Reveals Commensal Dysbiosis and an Association with Tenacibaculum dicentrarchi in AGD-Affected Atlantic Salmon (Salmo salar L.)

Metabolomic and metagenomic analyses of the Chinese mitten crab Eriocheir sinensis after challenge with Metschnikowia bicuspidata

Evaluation of sanitary risks associated with the consumption of street food in the city of Yaoundé (Cameroon): case of braised fish from Mvog-Ada, Ngoa Ekélé, Simbock, Ahala and Olézoa

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