Potential Role of Lipometabolism-Related MicroRNAs in Peripheral Blood Mononuclear Cells as Biomarkers for Coronary Artery Disease

Abstract

Aim: To explore the relationship between lipometabolism-related microRNAs (miRNAs) in peripheral blood mononuclear cells (PBMCs) and the presence of coronary artery disease (CAD).Methods: In the present study, 161 stable CAD patients and 149 health controls were enrolled. The expression levels of seven miRNAs (miR-21, miR-24, miR-29a, miR-33a, miR-34a, miR-103a, and miR-122) in PBMCs were qualified by quantitative real-time polymerase chain reaction (qRT-PCR). The miRNA markers that showed significant difference between the two groups were used for further analysis. The risk of miRNA contributing to the presence of CAD was estimated by univariate and multivariate logistic regression models. The area under the receiver operating characteristic curve (AUC) was used to evaluate diagnostic accuracy.Results: The expression levels of miR-24, miR-33a, miR-103a, and miR-122 in PBMCs were significantly increased in CAD patients compared with controls and were significantly correlated with blood lipids in both CAD patients and controls. The increased levels of miR-24 (adjusted OR = 1.32, 95% CI 1.07–1.62, P = 0.009), miR-33a (adjusted OR = 1.57, 95% CI 1.35–1.81, P < 0.001), miR-103a (adjusted OR = 1.01, 95% CI 1.01–1.02, P < 0.001), and miR-122 (adjusted OR = 1.03, 95% CI 1.01–1.04, P < 0.001) were associated with risk of CAD. We identified a miRNA panel (miR-24, miR-33, miR-103a, and miR-122) that provided a high diagnostic accuracy of CAD (AUC= 0.911, 95% CI 0.880–0.942).Conclusion: The increased expression levels of miR-24, miR-33a, miR-103a, and miR-122 in PBMCs are associated with risk of CAD. A panel of the four miRNAs has considerable clinical value in diagnosing stable CAD.

Global exosome transcriptome profiling reveals biomarkers for multiple sclerosis

“…However, circulating exosomes containing hsa‐miR‐122‐5p also originate from other cell types. Several recent reports have demonstrated a non–liver‐related role of miR‐122, for example, in the apoptosis of myocardium cells, in PBMCs during coronary artery disease, in hypothalamus, and in non–small‐cell lung cancer cells…”

“…25 However, circulating exosomes containing hsa-miR-122-5p also originate from other cell types. Several recent reports have demonstrated a non-liver-related role of miR-122, for example, in the apoptosis of myocardium cells, 26 in PBMCs during coronary artery disease, 27 in hypothalamus, 28 and in non-small-cell lung cancer cells. 29 hsa-miR-196b-5p, hsa-miR-301a-3p, and hsa-miR-532-5p have already been reported to be associated with immune cell function and to be related to inflammatory conditions.…”

Expression of miR‐18a and miR‐34c in circulating monocytes associated with vulnerability to type 2 diabetes mellitus and insulin resistance

“…A structured questionnaire was used to collect information on demographic data, environmental exposure and medical histories [12]. This study was approved by the university ethical committee, and informed consent was obtained from each participant.…”

A novel plasma lncRNA ENST00000416361 is upregulated in coronary artery disease and is related to inflammation and lipid metabolism

“…miRNA-33a and miRNA-33b are intron miRNAs encoded by the intron regions of SREBP1 and SREBP2 genes (48). miRNA-122 affects CAD by regulating genes associated with lipid synthesis and oxidation, including SREBP, microsomal triglyceride transfer protein, Krüppel-like factor 6 and cationic amino acid transporter 1 (49). Silencing of mirna-122 leads to a decrease in total cholesterol and triglyceride in plasma (49).…”