Potential Roles of Dec and Bmal1 Genes in Interconnecting Circadian Clock and Energy Metabolism

Sato, Fuyuki; Kohsaka, Akira; Bhawal, Ujjal K.; Muragaki, Yasuteru

doi:10.3390/ijms19030781

Cited by 62 publications

(42 citation statements)

References 129 publications

(202 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cells in peripheral tissues, including metabolic tissues such as liver, pancreas, and kidney, also display rhythms in activity that are regulated by an intrinsic clock machinery. Since metabolic events are tightly regulated by the circadian clock, disruptions of the circadian timing system can result in metabolic dysfunctions and contribute to obesity and type 2 diabetes (2)(3)(4)(5)(6)(7)(8)(9)(10). Thus, the intracellular clock system is tightly involved in the control of metabolic rhythms that are essential for metabolic homeostasis.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Rev-erbα Agonist SR9011 on the Immune Response and Cell Metabolism of Microglia

et al. 2020

View full text Add to dashboard Cite

Microglia are the immune cells of the brain. Hyperactivation of microglia contributes to the pathology of metabolic and neuroinflammatory diseases. Evidence has emerged that links the circadian clock, cellular metabolism, and immune activity in microglia. Rev-erb nuclear receptors are known for their regulatory role in both the molecular clock and cell metabolism, and have recently been found to play an important role in neuroinflammation. The Rev-erbα agonist SR9011 disrupts circadian rhythm by altering intracellular clock machinery. However, the exact role of Rev-erbα in microglial immunometabolism remains to be elucidated. In the current study, we explored whether SR9011 also had such a detrimental impact on microglial immunometabolic functions. Primary microglia were isolated from 1-3 days old Sprague-Dawley rat pups. The expression of clock genes, cytokines and metabolic genes was evaluated using RT-PCR and rhythmic expression was analyzed. Phagocytic activity was determined by the uptake capacity of fluorescent microspheres. Mitochondria function was evaluated by measuring oxygen consumption rate and extracellular acidification rate. We found that key cytokines and metabolic genes are rhythmically expressed in microglia. SR9011 disturbed rhythmic expression of clock genes in microglia. Pro-inflammatory cytokine expression was attenuated by SR9011 during an immune challenge by TNFα, while expression of the anti-inflammatory cytokine Il10 was stimulated. Moreover, SR9011 decreased phagocytic activity, mitochondrial respiration, ATP production, and metabolic gene expression. Our study highlights the link between the intrinsic clock and immunometabolism of microglia. We show that Rev-erbα is implicated in both metabolic homeostasis and the inflammatory responses in microglia, which has important implications for the treatment of metabolic and neuroinflammatory diseases.

show abstract

Section: Introductionmentioning

confidence: 99%

The Effect of Rev-erbα Agonist SR9011 on the Immune Response and Cell Metabolism of Microglia

et al. 2020

View full text Add to dashboard Cite

show abstract

“…SCN's "circadian clock" genes can control behavior, feeding, and reproduction through neurotransmitters and hormones ( Figure 1) [2,8]. These circadian clock genes drive the body's circadian rhythm, and their disruption can lead to a host of issues such as cancer, obesity, and atherosclerosis [3,[9][10][11][12][13][14][15][16]. Disruption of the clock genes has also been implicated in a variety of malfunctions of homeostasis, including glucose, and lipid metabolism [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Circadian Clock, Time-Restricted Feeding and Reproduction

Pan

Taylor

Cohen

et al. 2020

IJMS

View full text Add to dashboard Cite

The goal of this review was to seek a better understanding of the function and differential expression of circadian clock genes during the reproductive process. Through a discussion of how the circadian clock is involved in these steps, the identification of new clinical targets for sleep disorder-related diseases, such as reproductive failure, will be elucidated. Here, we focus on recent research findings regarding circadian clock regulation within the reproductive system, shedding new light on circadian rhythm-related problems in women. Discussions on the roles that circadian clock plays in these reproductive processes will help identify new clinical targets for such sleep disorder-related diseases.

show abstract

“…This heterodimer can activate transcription of PER1, PER2, PER3, CRY1 and CRY2 genes [36] . Defects in ARNTL have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns [37] . The BHLHE40 protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1 [38] .…”

Section: Discussionmentioning

confidence: 99%

Excavating thyroid carcinoma risk metabolic genes associated with biological clock by bioinformatics analysis: A study based on TCGA and GEO data

wang

Zhang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Thyroid carcinoma (TC) is malignant tumor of the endocrine gland with a significantly increased incidence in recent years, which related to metabolic diseases and biological clock disorder. The molecular mechanism and risk metabolic genes associated with biological clock of TC require conducting study. Methods We mined differentially expressed genes of TC (TC-DEGs) from TCGA and GEO database. Searching for biorhythm related genes (BRGs) based on core clock genes by STRING analysis. Next, we used a series of bioinformatics methods to analyze the TC-DEGs and BRGs, including chromosome location, GO and KEGG pathway annotation. Then, we extracted the expression of statistically significant TC metabolic genes by Perl program combined with a computational method of GSEA. Furthermore, screen the risk metabolic genes and reveal potential underlying KEGG pathways of the gene signatures. Results Our results identified 474 DEGs (P < 0.05; |log FC| > 1) in patients with TC compared with the normal controls using the available numerical mRNA expression values. The chromosomal assignments of TC-DEGs and BRGs were inhomogeneous. These genes were mainly distributed on Chrom01, 02, 11, 12, 04, 03 and X, none of the associated genes were distributed on sex Y chromosome. ARNTL and BHLHE40 belong to both the TC-DEGs and BRGs. PER2, NCOR1, RXRG, CCND1, SERPINE1 and PLAU were mined which act as the hub of signaling pathways linking “Thyroid hormone signaling pathway”, “Circadian rhythm”, “Transcriptional misregulation in cancer” and so on. One hundred and forty-one metabolic genes were extracted to train the prognostic model, and 11 risk genes were found, among them, 10 risk gene products have indirect interaction with ARNTL and BHLHE40. Such important genes for TC were regulated by many common conserved microRNAs. Conclusions Our study identified 8 key genes and 11 risk metabolic genes of thyroid carcinoma associated with biological clock by bioinformatics analysis. And the coding products of these genes form a clear interaction network, which is conducive to further study the molecular mechanism, reveal the role of circadian clock genes and metabolic genes in thyroid cancer.

show abstract

Potential Roles of Dec and Bmal1 Genes in Interconnecting Circadian Clock and Energy Metabolism

Cited by 62 publications

References 129 publications

The Effect of Rev-erbα Agonist SR9011 on the Immune Response and Cell Metabolism of Microglia

The Effect of Rev-erbα Agonist SR9011 on the Immune Response and Cell Metabolism of Microglia

Circadian Clock, Time-Restricted Feeding and Reproduction

Excavating thyroid carcinoma risk metabolic genes associated with biological clock by bioinformatics analysis: A study based on TCGA and GEO data

Contact Info

Product

Resources

About