Potential roles of extracellular vesicles as a noninvasive tool for prenatal genetic diagnosis

Saadeldin, Islam M.; Ehab, Seif; Swelum, Ayman A.

doi:10.1016/j.xfss.2023.01.002

Cited by 3 publications

(2 citation statements)

References 74 publications

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“…Shao-Chen Sun, Nanjing Agricultural University, China Some efforts have focused on using molecular markers from spent media and blastocoel fluid to assess the viability of the preimplantation embryo. Cell-free DNA and the contents of exosomes found in spent embryo culture media have all been studied to try and understand their specific origin from within the preimplantation embryo, and then attempting to clarify what these molecules tell us about the developing embryo (reviewed by Handayani et al, 2023;Saadeldin et al, 2023). The origin of cell-free DNA in the spent media and blastocoel fluid is thought to be from aneuploid cells that underwent apoptosis, yet there has been extensive research to use this cell-free DNA (non-invasive PGT-A) to assess embryo ploidy status that has yielded mixed results (reviewed by García-Pascual et al, 2023;Cinnioglu et al, 2023).…”

Section: Open Access Edited and Reviewed Bymentioning

confidence: 99%

Editorial: Molecular and cellular mechanisms in preimplantation IVF-embryo development

Chosed,

Kavoussi,

Berger

et al. 2023

Front. Cell Dev. Biol.

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Section: Open Access Edited and Reviewed Bymentioning

confidence: 99%

Editorial: Molecular and cellular mechanisms in preimplantation IVF-embryo development

Chosed,

Kavoussi,

Berger

et al. 2023

Front. Cell Dev. Biol.

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“…[9] In recent years, the potential clinical value of exosomal miRNAs in prenatal diagnostics has attracted increasing interest from researchers. [10] The integration of exosomal miRNAs analysis into prenatal diagnostics represents a promising advancement in noninvasive prenatal testing (NIPT). Moreover, the noninvasive nature of this approach minimizes risks to both the mother and the fetus.…”

Section: Introductionmentioning

confidence: 99%

Exosomal miRNAs in prenatal diagnosis: Recent advances

Jin,

Shen,

Shi

et al. 2024

Medicine

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Exosomes, small membranous microvesicles released by cells, contain a range of bioactive molecules, including proteins and miRNAs, which play critical roles in intercellular communication and physiological and pathological processes. Current research suggests that exosomal miRNAs could serve as valuable biomarkers for prenatal diseases, offering a noninvasive method for early detection and monitoring. Studies linking exosomal miRNAs to various birth defects, including fetal growth restriction, urinary tract malformations, cardiovascular system malformations, and hereditary diseases like Down syndrome, were discussed. However, there are some conflicting study findings due to different exosome separation methods. Here, we also discussed exosome separation methods, emphasizing the importance of method selection based on specific purposes and sample types. Further studies are needed to standardize isolation techniques, understand the specific mechanisms underlying exosomal miRNA function, and develop reliable noninvasive prenatal diagnostic indicators. Overall, exosomal miRNAs show promise as potential biomarkers for prenatal diagnosis, but further research is necessary to validate their clinical utility.

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Relevance of multilamellar and multicompartmental vesicles in biological fluids: understanding the significance of proportional variations and disease correlation

Saadeldin,

Ehab,

Cho

2023

Biomark Res

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Extracellular vesicles (EVs) have garnered significant interest in the field of biomedical science due to their potential applications in therapy and diagnosis. These vesicles participate in cell-to-cell communication and carry a diverse range of bioactive cargo molecules, such as nucleic acids, proteins, and lipids. These cargoes play essential roles in various signaling pathways, including paracrine and endocrine signaling. However, our understanding of the morphological and structural features of EVs is still limited. EVs could be unilamellar or multilamellar or even multicompartmental structures. The relative proportions of these EV subtypes in biological fluids have been associated with various human diseases; however, the mechanism remains unclear. Cryo-electron microscopy (cryo-EM) holds great promise in the field of EV characterization due to high resolution properties. Cryo-EM circumvents artifacts caused by fixation or dehydration, allows for the preservation of native conformation, and eliminates the necessity for staining procedures. In this review, we summarize the role of EVs biogenesis and pathways that might have role on their structure, and the role of cryo-EM in characterization of EVs morphology in different biological samples and integrate new knowledge of the alterations of membranous structures of EVs which could be used as biomarkers to human diseases.

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Potential roles of extracellular vesicles as a noninvasive tool for prenatal genetic diagnosis

Cited by 3 publications

References 74 publications

Editorial: Molecular and cellular mechanisms in preimplantation IVF-embryo development

Editorial: Molecular and cellular mechanisms in preimplantation IVF-embryo development

Exosomal miRNAs in prenatal diagnosis: Recent advances

Relevance of multilamellar and multicompartmental vesicles in biological fluids: understanding the significance of proportional variations and disease correlation

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