Potential serum biomarkers and metabonomic profiling of serum in ischemic stroke patients using UPLC/Q-TOF MS/MS

Abstract

BackgroundStroke still has a high incidence with a tremendous public health burden and it is a leading cause of mortality and disability. However, biomarkers for early diagnosis are absent and the metabolic alterations associated with ischemic stroke are not clearly understood. The objectives of this case-control study are to identify serum biomarkers and explore the metabolic alterations of ischemic stroke.MethodsMetabonomic analysis was performed using ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry and multivariate statistical analysis was employed to study 60 patients with or without ischemic stroke (30 cases and 30 controls).ResultsSerum metabolic profiling identified a series of 12 metabolites with significant alterations, and the related metabolic pathways involved glycerophospholipid, sphingolipid, phospholipid, fat acid, acylcarnitine, heme, and purine metabolism. Subsequently, multiple logistic regression analyses of these metabolites showed uric acid, sphinganine and adrenoyl ethanolamide were potential biomarkers of ischemic stroke with an area under the receiver operating characteristic curve of 0.941.ConclusionsThese findings provide insights into the early diagnosis and potential pathophysiology of ischemic stroke.

Serum Metabolomic Patterns in Young Patients with Ischemic Stroke

“…Previous studies have found that PC has many bene cial effects, including attenuating liver steatosis, slowing down aging-related processes, and improving brain function [36][37]. Consistent with previous research in older people, serum PCs were decreased in patients with ischemic stroke [33][34]. Moreover, the present study also found that young patients with ischemic stroke had increased PE (18:0/20:4, 16:0/22:5) levels and decreased levels of SM (d18:1/23:0, d20:0/19:1, d18:1/22:0, d16:0/26:1, d16:0/18:0, d16:0/22:1, d18:1/19:1, d16:0/17:1, d16:1/24:1, d18:1/19:0).…”

“…Several studies have demonstrated that lipid metabolites are associated with ischemic stroke [9,[33][34].…”

Metabolomics study on promoting blood circulation and ameliorating blood stasis: Investigating the mechanism of Angelica sinensis and its processed products

“…Sphingolipids is a class of lipids that, in addition to being involved in membrane domain formation (Simons & Gerl, ), is also a signaling molecule, which participates in cellular processes in animals (Hla & Dannenberg, ; Morad & Cabot, ; Van Echten‐Deckert & Walter, ). Recent studies have shown that abnormalities of sphingolipid metabolism were closely related to the occurrence of BSS, ischemic stroke and atherosclerosis (Gao, Ke, et al, ; Li et al, ; Sun, Zhao, Zhong, & Li, ). In this study, the plasma sphingomyelin, glucosylceramide and galactosylceramide, which involved in sphingolipid metabolism pathways, were significantly increased in BSS rats (Figure ).…”

Identification of potential diagnostic biomarkers of cerebral infarction using gas chromatography-mass spectrometry and chemometrics

“…MetaboAnalyst 3.0 (http://www.metaboanalyst.ca/) is a comprehensive tool suite for metabolomic data analysis, which combines the results from a powerful pathway enrichment analysis concerning the conditions under study and uses the high-quality KEGG pathway database as its back end knowledge base. 13 It was found that two metabolic pathways of arachidonic acid metabolism and fatty acid metabolism play important roles in the incidence of CI (Fig. 4).…”