Potential serum biomarkers associated with mild and severe leptospirosis infection: A cohort study in the Malaysian population

Tan, Xue Ting; Amran, Fairuz; Thayan, Ravindran; Ahmad, Norazah; Jaafar, Rosmina; Haron, Rahimah; Abdullah, Rafidah; Riffin, Nor Suhaila binti Md.; Abdul‐Rahman, Puteri Shafinaz

doi:10.1002/elps.201600471

Cited by 5 publications

(1 citation statement)

References 72 publications

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“…The response promotes host survival during the initial stages of injury and a return to homeostasis . A similar, well-defined group of APP proteins has been described in cats, dogs, ruminants, and humans. ,− A decreased synthesis of the proteins that decrease in abundance (negative APPs) may free up amino acids for the synthesis of the cytoprotective positive APPs. Albumin was the predominant negative APP in mice.…”

Section: Discussionmentioning

confidence: 99%

Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice

et al. 2021

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Performing large-scale plasma proteome profiling is challenging due to limitations imposed by lengthy preparation and instrument time. We present a fully automated multiplexed proteome profiling platform (AutoMP3) using the Hamilton Vantage liquid handling robot capable of preparing hundreds to thousands of samples. To maximize protein depth in single-shot runs, we combined 16-plex Tandem Mass Tags (TMTpro) with high-field asymmetric waveform ion mobility spectrometry (FAIMS Pro) and real-time search (RTS). We quantified over 40 proteins/min/sample, doubling the previously published rates. We applied AutoMP3 to investigate the naked mole-rat plasma proteome both as a function of the circadian cycle and in response to ultraviolet (UV) treatment. In keeping with the lack of synchronized circadian rhythms in naked mole-rats, we find few circadian patterns in plasma proteins over the course of 48 h. Furthermore, we quantify many disparate changes between mice and naked mole-rats at both 48 h and one week after UV exposure. These species differences in plasma protein temporal responses could contribute to the pronounced cancer resistance observed in naked mole-rats. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [1] partner repository with the dataset identifier PXD022891.

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Section: Discussionmentioning

confidence: 99%

Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice

et al. 2021

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Plasma proteome profiling reveals differentially expressed lipopolysaccharide-binding protein among leptospirosis patients

Fish-Low

Than

Ling

et al. 2020

Journal of Microbiology, Immunology and Infection

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The Potential of Eight Plasma Proteins as Biomarkers in Redefining Leptospirosis Diagnosis

Fish-Low,

Than,

Ling

et al. 2024

J. Proteome Res.

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Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing. This study investigated the plasma proteome of leptospirosis patients with leptospiraemia and seroconversion compared with dengue patients and healthy subjects using isobaric tags for relative and absolute quantitation (iTRAQ)-mass spectrometry (MS). The iTRAQ analysis identified a total of 450 proteins, which were refined to a list of 290 proteins through a series of exclusion criteria. Differential expression in the plasma proteome of leptospirosis patients compared to the control groups identified 11 proteins, which are apolipoprotein A-II (APOA2), C-reactive protein (CRP), fermitin family homolog 3 (FERMT3), leucine-rich alpha-2glycoprotein 1 (LRG1), lipopolysaccharide-binding protein (LBP), myosin-9 (MYH9), platelet basic protein (PPBP), platelet factor 4 (PF4), profilin-1 (PFN1), serum amyloid A-1 protein (SAA1), and thrombospondin-1 (THBS1). Following a study on a verification cohort, a panel of eight plasma protein biomarkers was identified for potential leptospirosis diagnosis: CRP, LRG1, LBP, MYH9, PPBP, PF4, SAA1, and THBS1. In conclusion, a panel of eight protein biomarkers offers a promising approach for leptospirosis diagnosis, addressing the limitations of the "one disease, one biomarker" concept.

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Potential serum biomarkers associated with mild and severe leptospirosis infection: A cohort study in the Malaysian population

Cited by 5 publications

References 72 publications

Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice

Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice

Plasma proteome profiling reveals differentially expressed lipopolysaccharide-binding protein among leptospirosis patients

The Potential of Eight Plasma Proteins as Biomarkers in Redefining Leptospirosis Diagnosis

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