Potential stem cell—Conditioned medium and their derived exosomes versus omeprazole in treatment of experimental model of gastric ulcer

Ibrahim, Manar Fouli Gaber; Allam, Fatma Alzhraa Fouad Abdelbaki

doi:10.1016/j.acthis.2022.151896

Cited by 7 publications

(2 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The normal gastric mucosa was complete, and the mucosal layer was covered with a single layer of columnar epithelium. There was no obvious hyperplasia in the gastric mucosa with no inflammatory cell infiltration in the muscularis and adventitia, while broken mucosa with severe hemorrhage (red arrow) and inflammatory cell infiltration (green arrow) appeared in the H&E-stained stomach tissues induced by ethanol in the model group as previously reported by other studies. , Empty NPs did not exhibit any noticeable improvement in pathological tissue, with hemorrhage, neutrophils (green arrows), and lymphocytes (blue arrows) still present. In the OME group, the gastric tissue was also damaged with superficial degeneration, necrosis of the mucosal layer in some areas (black arrow), and loose connective tissue.…”

Section: Resultsmentioning

confidence: 99%

Bletilla striata Polysaccharide Nanoparticles Improved the Therapeutic Efficacy of Omeprazole on the Rat Gastric Ulcer Induced by Ethanol

Jing

Yang

et al. 2023

Mol. Pharmaceutics

View full text Add to dashboard Cite

Gastric ulcers are a common clinical presentation affecting anyone, regardless of their age or gender. Nanoparticles (NPs) containing Bletilla striata polysaccharide (BSP) and omeprazole (OME) were investigated in the study for their therapeutic effect on gastric ulcers. Ethanol-induced gastric ulcers in rats (240 ± 30 g) were established. Our OME-BSP NPs were more stable than free OME in the acidic environment and can increase the absorption of OME in rat stomach, which was confirmed by in situ gastric absorption and distribution experiments. The extended blood circulation of OME-BSP NPs was also observed in rats with gastric ulcer. More importantly, OME-BSP NPs not only decreased the area of gastric ulcer and inhibited gastric acid secretion but also reversed gastric tissue damage and cell apoptosis, as revealed by HE and TUNEL staining. Subsequent SOD, MDA, PGE2, IL-6, and TNF-α tests further verified the superiority of OME-BSP NPs against rat gastric ulcer, which properly originated from superior antioxidant and anti-inflammatory effects. As a result, our OME-BSP NPs’ drug delivery system improved the stability and absorption of OME in the rat stomach and achieved targeted treatment of gastric ulcers.

show abstract

Section: Resultsmentioning

confidence: 99%

Bletilla striata Polysaccharide Nanoparticles Improved the Therapeutic Efficacy of Omeprazole on the Rat Gastric Ulcer Induced by Ethanol

Jing

Yang

et al. 2023

Mol. Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…The exosomes and conditioned medium derived from bone marrow mesenchymal stem cells were used to cure gastric ulcers induced by aspirin in rats. The exosomes restored the tissue structure of gastric mucosa to a more normal state [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Mesenchymal Stem Cells Accelerate Recovery of Acetic Acid‐Induced Chronic Gastric Ulcer by Regulating Ekt/Akt/TRIM29 Axis

Zhao,

Fan,

Jia

et al. 2024

Stem Cells International

View full text Add to dashboard Cite

Chronic gastric ulcer (CGU), a prevalent digestive disease, has a high incidence and is seriously harmful to human health. Mesenchymal stem cells (MSCs) have been proven to have beneficial therapeutic effects in many human diseases. Here, a CGU model induced by acetic acid in mice was used to evaluate the repair effects and potential mechanism of human umbilical cord-derived MSCs (hUC-MSCs) and hUC-MSCs derived conditioned medium (hUC-MSC-CM). We found that hUC-MSCs and hUC-MSC-CM treatment significantly repaired morphological characteristics of CGU, improved proliferation and decreased apoptosis of gastric cells, and promoted the generation of new blood vessels in granulation tissues. In addition, we could detect the homing of MSCs in gastric tissue, and MSCs may differentiate into Lgr5-positive cells. As well as this, in vitro experiments showed that hUC-MSC-CM could promote cell proliferation, stimulate cell cycle progression, and reduce the incidence of apoptosis. The transcriptome of cells and the iTRAQ proteome of gastric tissues suggest that MSCs may play a therapeutic role by increasing the expression of TRIM29. Additionally, it was found that knocking down TRIM29 significantly decreased the ameliorative effects of hUC-MSC-CM on cell apoptosis. As a result of further molecular experiments, it was found that TRIM29 is capable of phosphorylating Erk/Akt in specific cell type. As a whole, it appears that hUC-MSCs can be an effective therapeutic approach for promoting gastric ulcer healing and may exert therapeutic effects in the form of paracrine and differentiation into gastric cells.

show abstract

Oxidative stress and NF-KB/iNOS inflammatory pathway as innovative biomarkers for diagnosis of drowning and differentiating it from postmortem submersion in both fresh and saltwater in rats

Adel,

Ibrahim,

Elsayed

et al. 2024

Int J Legal Med

View full text Add to dashboard Cite

Background Finding a dead body in water raises an issue concerning determining the cause of death as drowning because of the complex pathophysiology of drowning. In addition, the corpse may be submersed postmortem. Objective Evaluate the role of oxidative stress markers and NF-KB/iNOS inflammatory pathway as diagnostic biomarkers in drowning and whether they could differentiate freshwater from saltwater drowning. Methods This study included forty-five adult male albino rats classified into five groups: control group (C), Freshwater-drowned group (FD), Freshwater postmortem submersion group (FPS), saltwater-drowned group (SD), and saltwater postmortem submersion group (SPS). After the autopsy, the rats' lungs in each group were prepared for histological, immunohistochemical (caspase 3, TNF-α, NF-kB, COX-2 & iNOS), biochemical studies; MDA, NOx, SOD, GSH, VCAM-1, COX-2; and RT-PCR for the relative quantification of NF-kB and iNOS genes expression. Results Lung oxidative markers were significantly affected in drowned groups than in postmortem submersion groups. Inflammatory pathway markers were also significantly increased in the drowned groups, with concern that all markers were significantly affected more in saltwater than in freshwater drowned group. Conclusions It is concluded that the tested markers can be used accurately in diagnosing drowning and differentiating it from postmortem submersion with a better understanding of the mechanism of death in drowning as both mechanisms, inflammatory and oxidative stress, were revealed and involved.

show abstract

Potential stem cell—Conditioned medium and their derived exosomes versus omeprazole in treatment of experimental model of gastric ulcer

Cited by 7 publications

References 58 publications

Bletilla striata Polysaccharide Nanoparticles Improved the Therapeutic Efficacy of Omeprazole on the Rat Gastric Ulcer Induced by Ethanol

Bletilla striata Polysaccharide Nanoparticles Improved the Therapeutic Efficacy of Omeprazole on the Rat Gastric Ulcer Induced by Ethanol

Mesenchymal Stem Cells Accelerate Recovery of Acetic Acid‐Induced Chronic Gastric Ulcer by Regulating Ekt/Akt/TRIM29 Axis

Oxidative stress and NF-KB/iNOS inflammatory pathway as innovative biomarkers for diagnosis of drowning and differentiating it from postmortem submersion in both fresh and saltwater in rats

Contact Info

Product

Resources

About