Potential Theranostics Application of Bio-Synthesized Silver Nanoparticles (4-in-1 System)

Abstract

In this report, we have designed a simple and efficient green chemistry approach for the synthesis of colloidal silver nanoparticles (b-AgNPs) that is formed by the reduction of silver nitrate (AgNO3) solution using Olax scandens leaf extract. The colloidal b-AgNPs, characterized by various physico-chemical techniques exhibit multifunctional biological activities (4-in-1 system). Firstly, bio-synthesized silver nanoparticles (b-AgNPs) shows enhanced antibacterial activity compared to chemically synthesize silver nanoparticles (c-AgNPs). Secondly, b-AgNPs show anti-cancer activities to different cancer cells (A549: human lung cancer cell lines, B16: mouse melanoma cell line & MCF7: human breast cancer cells) (anti-cancer). Thirdly, these nanoparticles are biocompatible to rat cardiomyoblast normal cell line (H9C2), human umbilical vein endothelial cells (HUVEC) and Chinese hamster ovary cells (CHO) which indicates the future application of b-AgNPs as drug delivery vehicle. Finally, the bio-synthesized AgNPs show bright red fluorescence inside the cells that could be utilized to detect the localization of drug molecules inside the cancer cells (a diagnostic approach). All results together demonstrate the multifunctional biological activities of bio-synthesized AgNPs (4-in-1 system) that could be applied as (i) anti-bacterial & (ii) anti-cancer agent, (iii) drug delivery vehicle, and (iv) imaging facilitator. To the best of our knowledge, there is not a single report of biosynthesized AgNPs that demonstrates the versatile applications (4-in-1 system) towards various biomedical applications. Additionally, a plausible mechanistic approach has been explored for the synthesis of b-AgNPs and its anti-bacterial as well as anti-cancer activity. We strongly believe that bio-synthesized AgNPs will open a new direction towards various biomedical applications in near future.

Immunomodulatory properties of silver nanoparticles contribute to anticancer strategy for murine fibrosarcoma

“…2 Silver nanoparticles (AgNPs) are among the most commercialized nanoparticles because of their unique optical, catalytic and disinfectant properties. Currently, AgNPs are being used for many different applications, [3][4][5] ranging from wound dressing and the coatings of surgical instruments and prostheses 6,7 to the use in food container systems or as the coating material for certain household devices, such as washing machines. AgNPs are being incorporated into textiles and are also added to cosmetics.…”

Aspartame-Stabilized Gold–Silver Bimetallic Biocompatible Nanostructures with Plasmonic Photothermal Properties, Antibacterial Activity, and Long-Term Stability

“…Therefore, it is the combi- indicating that possible uptake mechanisms may take place in this case, as it does for AgNP. 9 However, both strains present symptoms of membrane structural damage, which is likely attributed to the antibacterial properties of silver. In conclusion, bimetallic core-shell nanoparticles allow for the exploitation of properties characterstic for monometallic (Au and Ag) nanoparticles.…”

“…This property has been proposed as a powerful tool for photodynamic therapy (PDT), a light-mediated 20 form of clinical treatment; 4,7 (b) Silver nanoparticles (AgNP) have excellent antimicrobial properties that exceed those of silver ions themselves, and it has been proposed that the AgNP themselves are strongly anti-infective, beyond any effect due to Ag + leaching 8,9 and (c) peptides, such as LL37 can stabilize metal nanoparticles, such as AgNP, 25 while they retain their antiinfective properties and showing excellent biocompatibility. 10 Based on this observation, we present here the remarkable behavior of core-shell gold-silver nanostructures stabilized with aspartame (Asm), a common artificial sweetener.…”

Hybrid nanomaterial: biocolloidals

“…For example, specific protein molecules and peptide structures, tertiary protein molecules (Jang and Kim, 2011;De Backer et al, 2015) (integrated with surfactants), casein (Tielemans et al, 2006;Qiu et al, 2012;Seisenbaeva et al, 2012;Yasun et al, 2015), various ionic structures, or two-faced peptides (Breitling et al, 2009;Coppage et al, 2012;Knez et al, 2013) will form self-assembled nanoscale structures in laboratory conditions (Newcomb et al, 2012). Protein cages offer different interior and exterior functional regions that can be utilized for different modifications and are one of the polypeptide-based materials that are increasingly being offered for bioapplications (Bhattacharya and Mukherjee, 2008;Mukherjee et al, 2014;Devetter et al, 2015;Kumar et al, 2015). Other commonly known protein structures involve heat shock proteins, ferritin, and viral-coat materials.…”