Potential therapeutic approaches of microRNAs for COVID-19: Challenges and opportunities

Farshbaf, Alieh; Mohtasham, Nooshin; Zare, Reza; Mohajertehran, Farnaz; Rezaee, Seyed Abdolrahim

doi:10.1016/j.jobcr.2020.12.006

Cited by 13 publications

(10 citation statements)

References 66 publications

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“…The COVID‐19‐encoded miRNAs as autocrine or paracrine agonists of host cells increased nuclear factor kappa B activity and stimulated proinflammatory cytokines (Arisan et al, 2020 ). Therefore, further investigation is required to identify specific miRNAs that target and stop virus‐induced infection (Farshbaf et al, 2020 ; Pontecorvi et al, 2020 ).…”

Section: Limitations Of Mirnas Against Covid‐19mentioning

confidence: 99%

miRNAs; a novel strategy for the treatment of COVID‐19

Hao

Ebrahimi

Keivan

et al. 2021

Cell Biology International

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Coronavirus disease 2019 (COVID‐19) is the seventh member of the bat severe acute respiratory syndrome family. COVID‐19 can fuse their envelopes with the host cell membranes and deliver their genetic material. COVID‐19 attacks the respiratory system and stimulates the host inflammatory responses, enhances the recruitment of immune cells, and promotes angiotensin‐converting enzyme 2 activities. Patients with confirmed COVID‐19 may have experienced fever, dry cough, headache, dyspnea, acute kidney injury, acute respiratory distress syndrome, and acute heart injury. Several strategies such as oxygen therapy, ventilation, antibiotic or antiviral therapy, and renal replacement therapy are commonly used to decrease COVID‐19‐associated mortality. However, these approaches may not be good treatment options. Therefore, the search for an alternative‐novel therapy is urgently important to prevent the disease progression. Recently, microRNAs (miRNAs) have emerged as a promising strategy for COVID‐19. The design of oligonucleotide against the genetic material of COVID‐19 might suppress virus RNA translation. Several previous studies have shown that host miRNAs play an antiviral role and improve the treatment of patients with COVID‐19. miRNAs by binding to the 3′‐untranslated region (UTR) or 5′‐UTR of viral RNA play an important role in COVID‐19‐host interplay and viral replication. miRNAs interact with multiple pathways and reduce inflammatory biomarkers, thrombi formation, and tissue damage to accelerate the patient outcome. The information in this review provides a summary of the current clinical application of miRNAs for the treatments of patients with COVID‐19.

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Section: Limitations Of Mirnas Against Covid‐19mentioning

confidence: 99%

miRNAs; a novel strategy for the treatment of COVID‐19

Hao

Ebrahimi

Keivan

et al. 2021

Cell Biology International

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“…SARS-CoV-2 encoded miRNAs alter host gene expression to create a favorable environment for viral infection. Interestingly, host miRNAs can play dual roles as both antiviral and proviral factors [ 45 ]. The number of host miRNAs targeting the SARS-CoV-2 genome sequence inhibits viral replication by regulating innate antiviral immunity [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

“…Aydemir et al have identified 20 pre-miRNA candidates and 40 mature miRNAs encoded by SARS-CoV-2, which can regulate host gene expression by targeting NFKB, JAK/STAT, and TGFB signaling pathways [ 44 ] ( Table 2 ). Therefore, viral miRNAs, such as SARS-CoV-mir-D8- 5p and SARS-CoV-mir-R1-5p, target many human genes involved in the transcription, metabolism, and immune systems, implicating the roles of miRNAs during viral infection [ 44 , 45 ]. Likewise, predicted 26 mature miRNAs from the SARS-CoV-2 genome might target human genes involved in innate antiviral immunity [ 46 ].…”

Section: Methodsmentioning

confidence: 99%

Interplays between human microbiota and microRNAs in COVID-19 pathogenesis: a literature review

Hong

Kim

2021

Phys Act Nutr

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[Purpose] Recent studies have shown that COVID-19 is often associated with altered gut microbiota composition and reflects disease severity. Furthermore, various reports suggest that the interaction between COVID-19 and host-microbiota homeostasis is mediated through the modulation of microRNAs (miRNAs). Thus, in this review, we aim to summarize the association between human microbiota and miRNAs in COVID-19 pathogenesis.[Methods] We searched for the existing literature using the keywords such “COVID-19 or microbiota,” “microbiota or microRNA,” and “COVID-19 or probiotics” in PubMed until March 31, 2021. Subsequently, we thoroughly reviewed the articles related to microbiota and miRNAs in COVID-19 to generate a comprehensive picture depicting the association between human microbiota and microRNAs in the pathogenesis of COVID-19.[Results] There exists strong experimental evidence suggesting that the composition and diversity of human microbiota are altered in COVID-19 patients, implicating a bidirectional association between the respiratory and gastrointestinal tracts. In addition, SARS-CoV-2 encoded miRNAs and host cellular microRNAs modulated by human microbiota can interfere with viral replication and regulate host gene expression involved in the initiation and progression of COVID-19. These findings suggest that the manipulation of human microbiota with probiotics may play a significant role against SARS-CoV-2 infection by enhancing the host immune system and lowering the inflammatory status.[Conclusion] The human microbiota-miRNA axis can be used as a therapeutic approach for COVID-19. Hence, further studies are needed to investigate the exact molecular mechanisms underlying the regulation of miRNA expression in human microbiota and how these miRNA profiles mediate viral infection through host-microbe interactions.

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“…Similar DNA lengths are commonly exploited for PCR primers, or in structural DNA-nanotechnology applications [26]. Moreover, 23 bases represents the average length of micro-RNA (miRNA) biomarkers, a class of naturally occurring, small, noncoding RNA molecules, which are the target of several microarrays or biosensors [23,27]. The sequence of the surface-immobilized probe was: 5 -GCCCACCTATAAGGTAAAAGTGA-3 .…”

Section: Dna Strands and Reagentsmentioning

confidence: 99%

Copolymer Coatings for DNA Biosensors: Effect of Charges and Immobilization Chemistries on Yield, Strength and Kinetics of Hybridization

et al. 2021

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The physical–chemical properties of the surface of DNA microarrays and biosensors play a fundamental role in their performance, affecting the signal’s amplitude and the strength and kinetics of binding. We studied how the interaction parameters vary for hybridization of complementary 23-mer DNA, when the probe strands are immobilized on different copolymers, which coat the surface of an optical, label-free biosensor. Copolymers of N, N-dimethylacrylamide bringing either a different type or density of sites for covalent immobilization of DNA probes, or different backbone charges, were used to functionalize the surface of a Reflective Phantom Interface multispot biosensor made of a glass prism with a silicon dioxide antireflective layer. By analyzing the kinetic hybridization curves at different probe surface densities and target concentrations in solution, we found that all the tested coatings displayed a common association kinetics of about 9 × 104 M−1·s−1 at small probe density, decreasing by one order of magnitude close to the surface saturation of probes. In contrast, both the yield of hybridization and the dissociation kinetics, and hence the equilibrium constant, depend on the type of copolymer coating. Nearly doubled signal amplitudes, although equilibrium dissociation constant was as large as 4 nM, were obtained by immobilizing the probe via click chemistry, whereas amine-based immobilization combined with passivation with diamine carrying positive charges granted much slower dissociation kinetics, yielding an equilibrium dissociation constant as low as 0.5 nM. These results offer quantitative criteria for an optimal selection of surface copolymer coatings, depending on the application.

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Potential therapeutic approaches of microRNAs for COVID-19: Challenges and opportunities

Cited by 13 publications

References 66 publications

miRNAs; a novel strategy for the treatment of COVID‐19

miRNAs; a novel strategy for the treatment of COVID‐19

Interplays between human microbiota and microRNAs in COVID-19 pathogenesis: a literature review

Copolymer Coatings for DNA Biosensors: Effect of Charges and Immobilization Chemistries on Yield, Strength and Kinetics of Hybridization

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