This study was conducted to determine the effect of four weeks of high intensity interval training on blood oxygen saturation, body composition and some performance parameters in young male football players. Pre-test-post-test control group experimental method was used in the study. Twelve experimental (age: 16,33,77 years; height: 171,50,6,23 cm; body weight: 58,33,5,19 kg) and 12 control (age: 16,50,52 years; height: 168,58,5,68 cm; body weight: 58,05,4,60 kg) male football players aged 15-17 years, who regularly exercise and have been playing football for at least 3 years participated in the study. In the study, the experimental group performed high intensity interval training 3 days a week in addition to football training for 4 weeks. The control group only performed routine football training. In the study, height and body weight, resting heart rate, systolic and diastolic blood pressure, blood oxygen saturation, body composition, 20 m sprint test, Illinois agility test and Yo-Yo interval running test were applied as pre and post tests. The normality level of the data was determined by Shapiro-Wilk test. Independent Sample T Test for independent groups and Paired Sample T Test for dependent groups were used to analyse the normally distributed data. According to the findings of the study, while statistical significance was determined in blood oxygen saturation, body composition, Illinois agility test and Yo-Yo interval running parameters of the experimental group (p<0.05), no significant difference was found in 20 m sprint test (p>0.05). According to the results of the study, it can be said that 4-week high intensity interval training has positive effects on blood oxygen saturation, body composition and performance parameters in young male football players.