Potential Use of Biological Herbicides in a Circular Economy Context: A Sustainable Approach

Stefanski, Fábio Spitza; Camargo, Aline Frumi; Scapini, Thamarys; Bonatto, Charline; Venturin, Bruno; Weirich, Sabrina Natalia; Ulkovski, Cleiton; Carezia, Carine; Ulrich, Alessandro; Michelon, William; Soares, Hugo Moreira; Mathiensen, Alexandre; Fongaro, Gislaine; Mossi, Altemir José; Treichel, Helen

doi:10.3389/fsufs.2020.521102

Cited by 20 publications

(20 citation statements)

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“…Fermentation was carried out in an Erlenmeyer with a practical volume of 100 mL under the conditions previously optimized by Stefanski et al 14 . to produce the target enzymes.…”

Section: Methodsmentioning

confidence: 99%

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Klanovicz

Stefanski

Camargo

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND Our study aimed to characterize and prospect immobilization strategies for a novel fungal peroxidase (POD) and insert it in pollutant remediation context. The enzymatic extract was obtained by submerged fermentation of Trichoderma koningiopsis in an alternative substrate consisting of fresh microalgal biomass. The immobilization efficiency was evaluated by monitoring the residual activity (RA) and the discoloration potential (DP) of a synthetic dye solution. Concomitantly, the POD catalytic properties were explored, and the most promising storage strategy to maintain the enzymatic activity was studied. RESULTS The novel and non‐purified guaiacol peroxidase from T. koningiopsis expressed a specific activity of up to 7801 U mg−1 in the free form, showing stability when subjected to up to 80 °C in a pH range between 4.0 and 8.0. Furthermore, the bioproduct immobilized on Fe3O4 magnetic nanoparticles, named magnetic nanozymes (MN‐POD), expressed up to 689% RA and 100% removal for the Direct Brown 27 dye. An increase in the enzymatic activity, in both free and immobilized forms, was also observed after storage for up to 8 months. The synthesized magnetic nanozymes showed good reusability, maintaining 13 546 U mg−1 after 10 cycles, and removing 94% of color in a second batch. Toxicological evaluation with Allium cepa indicated that the enzymatic discoloration process with immobilized POD was essential for eliminating genotoxic effects. CONCLUSION The T. koningiopsis peroxidase production and immobilization presented in this work are promising for the enzyme market and for wastewater treatment technologies due to their high bioxidative potential. © 2022 Society of Chemical Industry (SCI).

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“…Fermentation was carried out in an Erlenmeyer with a practical volume of 100 mL under the conditions previously optimized by Stefanski et al 14 . to produce the target enzymes.…”

Section: Methodsmentioning

confidence: 99%

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Klanovicz

Stefanski

Camargo

et al. 2022

J of Chemical Tech & Biotech

Self Cite

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“…As well as the fungus fermentation process, the microalgae growth process occurred in a stressful environment, considering that it was cultivated with mainly ammonia and phosphorus availability (Michelon et al, 2016). Thus, according to a previous study (Stefanski et al, 2020), synergism between fungi and microalgae occurs, resulting in microalgae cells incorporated into the fungal hyphae mesh. This synergy may have led to the obtention of an enzymatic extract with high resilience capacity in peroxidase activity, which can be seen in Figure 1, where the interaction between the enzyme and reaction medium over time is shown under constant environmental conditions.…”

Section: Catalytic Properties Of Free and Crude Peroxidasementioning

confidence: 95%

“…Crude extracts with different peroxidase:laccase ratio, obtained in previous studies (Stefanski et al, 2020) varying operational parameters in fermentation, were applied in the dye removal study. The tests were carried out at a volume of 100 mL under constant agitation (160 rpm) and temperature (25 °C) for 5 h. The DP was quanti ed by the difference between initial (Abs i ) and nal (Abs f ) absorbances of the treated samples (Equation 1).…”

Section: Discoloration Studiesmentioning

confidence: 99%

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Klanovicz

Stefanski

Camargo

et al. 2021

Preprint

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The occurrence of micropollutants in aqueous matrices has become a global concern and a challenge for wastewater treatment plants. Monitoring their toxicity has shown that these compounds, even at low concentrations, pose risks to human and environmental health. Therefore, our study aimed to prospect immobilization strategies for non-commercial oxidoreductase enzymes and insert them in the context of pollutant remediation. The enzymatic extract was obtained by submerged fermentation of the fungus Trichoderma koningiopsis in an alternative substrate, consisting of fresh microalgal biomass from the phycoremediation process. The immobilization efficiency of peroxidase (POD) was evaluated by monitoring the residual activity (RA) and the discoloration potential (DP) of a synthetic dye solution. Concomitantly, the catalytic properties of free POD were explored, and the most promising storage strategy to maintain the enzymatic activity was studied. Guaiacol peroxidase from T. koningiopsis expressed specific activity of up to 7801.1 U mg− 1 in the free form, showing stability when subjected to up 80°C in a pH range between 4.0–8.0. Furthermore, the bioproduct immobilized on magnetic nanoparticles expressed up to 688% RA and 100% DP. An increase in the RA of the enzyme, both in free and immobilized form, was also observe after storage for up to 8 months. The synthesized magnetic nanozymes showed good reusability, maintaining 13546.4 U mg− 1 after ten cycles and removing 93% of color in a second batch. Toxicological evaluation with Allium cepa indicated that the enzymatic process of color removal with immobilized POD, despite maintaining unwanted cytotoxic effects, was essential to eliminate genotoxic effects. In this sense, the immobilization processes of T. koningiopsis peroxidase presented in our work are promising for the enzyme market and for the wastewater treatment sector.

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“…Their role is to attack specific weeds through the spread of diseases on them. In fact, the use of bioherbicides is especially important given the willingness to reinforce the circular economy; indeed, agricultural wastes could be used (e.g., as green manure) only if they are free of synthetic herbicides [31]. However, bioherbicides are few in number, have a narrow spectrum of use, and are hardly commercialized [31,32].…”

Section: Bioherbicides: Sustainable Circular But With Low Profitability and Efficiencymentioning

confidence: 99%

“…In fact, the use of bioherbicides is especially important given the willingness to reinforce the circular economy; indeed, agricultural wastes could be used (e.g., as green manure) only if they are free of synthetic herbicides [31]. However, bioherbicides are few in number, have a narrow spectrum of use, and are hardly commercialized [31,32]. Indeed, weed resistance and high production costs are serious challenges to overcome for the development of bioherbicides.…”

Section: Bioherbicides: Sustainable Circular But With Low Profitability and Efficiencymentioning

confidence: 99%

Sustainability, Efficiency, and Circularity of Weedy Rice Management Strategies

Svizzero

2021

Circ.Econ.Sust.

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Weeds have always been a serious problem for farmers and especially nowadays given the current challenges related to food security and agriculture sustainability. During the last three decades, the increasing scarcity of labor, energy, and water has led to a switch of the rice establishment method from traditional hand transplanting to direct seeding. While the latter presents some advantages, it also fosters weeds among which weedy rice is considered the worst because it has a strong competitive ability, and as a congeneric of cultivated rice is very difficult to control. There are currently three main weed management strategies in rice: synthetic herbicides, herbicide-resistant rice varieties, and integrated weed management (IWM). However, all these strategies have low effectiveness and sustainability. Even though IWM is strongly recommended, its adoption remains very low owing to its complexity and the additional cost it induces. The use of crop rotation and cover crops is sustainable and consistent with the circularity principles, but this strategy presents the same drawbacks than those associated with IWM. We stress that other strategies used to control or suppress weedy rice are more efficient, sustainable, and consistent with the bioeconomy principles. They encompass the control of the pathways to weediness by ferality as well as the improvement of rice cultivars' fitness based on allelopathic effects. Other non-chemical weed management strategies, such as the use of bioherbicides, are promising given the current transition towards bioeconomy and circular economy.

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Potential Use of Biological Herbicides in a Circular Economy Context: A Sustainable Approach

Cited by 20 publications

References 64 publications

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Complete wastewater discoloration by a novel peroxidase source with promising bioxidative properties

Sustainability, Efficiency, and Circularity of Weedy Rice Management Strategies

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