Potential use of lactic acid bacteria Leuconostoc mesenteroides as a probiotic for the removal of Pb(II) toxicity

Abstract

It has been demonstrated that certain lactic acid bacteria (LAB) can sequester metal ions by binding them to their surfaces. In the present study, lead (Pb)-resistant LAB were isolated from kimchi, a Korean fermented food. A total of 96 different LAB strains were isolated, and 52 strains showed lead resistance. Among them, an LAB strain-96 (L-96) identified as Leuconostoc mesenteroides showed remarkable Pb resistance and removal capacity. The maximum adsorption capacity of this strain calculated using the Langmuir isotherm was 60.6 mg Pb/g. In an in vivo experiment, young male mice were provided with water (A), Pb-water (B), or Pb-water+ L-96 (C) during puberty. Lower glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) levels in Pb-exposed male mice that received strain L-96 as a probiotic were suggestive of reduced hepatotoxicity. Moreover, feces from mice treated with L-96 contained more Pb than feces from untreated mice. Increased Pb elimination likely reduced internal accumulation, and this hypothesis was supported by significantly lower Pb concentrations in kidneys and testes of the mice treated with strain L-96. The motility and ATP content of epididymal spermatozoa were partially restored if strain L-96 was administered. In conclusion, isolated L-96 LAB had lead-biosorption activity and efficiently detoxified lead-poisoned male mice, resulting in recovering male reproductive function. These results suggest the potential use of LAB as a probiotic to protect humans from the adverse effects of Pb exposure.

Assessment of the heavy metal bioremediation efficiency of the novel marine lactic acid bacterium, Lactobacillus plantarum MF042018

“…Where; "C e " is the metal concentration at equilibrium. Both the Langmuir and the Freundlich isotherm models were applied to the metal uptake data of the LAB isolates as described by Khan et al 39 and Yi et al 35 . The Langmuir isotherm model is represented using the following equation;…”

“…In contrast, Halttunen et al 34 reported significant increase in Cd removal by Lactobacillus rhamnosus GG when the incubation temperature was raised to 37 °C. Furthermore, Yi et al 35 and Halttunen et al 14 indicated that, for in vivo practical use, LAB perform high Pb removal capacity at low pH that could be due to a higher number of phosphate groups available on the bacterial surface. Similarly, Hansen et al 36 revealed that acidic pH was optimum for maximum removal of As(V) by Lessonia nigrescens.…”

Biosorption of cadmium by potential probiotic Pediococcus pentosaceus using in vitro digestion model

“…Some studies have shown that in addition to conventional chelation therapy for cadmium poisoning, the use of microorganism‐based dietary therapy offers more effective and safer for patients. Over the past two decades, several microorganisms such as Pseudomonas , Streptococcus , Bacillus , and lactic acid bacteria (LAB) have been reported to exhibit metal‐binding abilities. However, using these microorganisms as a therapeutic strategy against Cd toxicity contains major issues because of safeguarding humans and tolerance to extreme environments in the human gut.…”

Dynamique des Bactéries Lactiques des ferments traditionnels de manioc (<i>Manihot esculenta, Crantz</i>) destinés à la production de l’attiéké <i>Adjoukrou, Ahizi</i> et <i>Ebrié</i>, en Côte d’Ivoire

“…Leuconostoc mesenteroides qui constitue la particularité de la production de l'attiéké Ebrié sur les sites étudiés dans la ville d'Abidjan, est impliquée dans de le processus de fermentation pour la production d'aliments fermentés tels que le kim-chi et la choucroute. Cette espèce de bactérie lactique est même incluse dans divers starters en panification et production laitière (Yi et al;Lee et al, 2015 ;De Paula et al;2014;Oyedeji et al;Otgonbayar et al, 2011 ). En outre, les Leuconostoc, sont utilisées comme starter pour leur aptitude à produire des exo-polysaccharides (dextrane ou levane), des oligosaccharides, du mannitol, des bactériocines et des vitamines (Shin et Han, 2015).…”