Potential Utilization of Pineapple Waste Streams for Polyhydroxyalkanoates (PHAs) Production via Batch Fermentation

Abstract

This study involves the production of a value-added product of polyhydroxyalkanoates (PHAs) from pineapple cannery wastes by isolated bacterial strain of Bacillus sp. SV13 via batch fermentation process. Two carbon sources, glucose and sucrose, were used to investigate the potential of PHAs production compared to when the agro-industrial waste was used. Batch fermentation was performed in a flask scale under controlled conditions: 37°C, and 200 rpm of agitation rate. The PHAs concentration [g/L], biomass in the forms of dried cell weight (DCW) [g/L] and specific product yield (Yp/x) were determined by two-way analysis of variance (two-way ANOVA). The results revealed that PHAs concentration, biomass, and Yp/x obtained from pineapple waste were significantly greater than the results obtained from glucose and sucrose. The Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (NMR) techniques were used to examine the chemical structure of PHAs. It was found that the extracted PHAs remarkably showed in the form of polyhydroxybutyrate (PHB).

Valorization of Pineapple Peel Waste for Sustainable Polyhydroxyalkanoates Production

“…1, and the filtrate was collected and finally adjusted pH to 7.0 to obtain pineapple peel hydrolysate (PPH) containing fermentable sugars. The compositions and concentration of the sugars in PPH were analyzed using highperformance liquid chromatography (HPLC) with refractive index detector (RID), Zorbax NH 2 column, mobile phase: acetonitrile/water (75−25% v/v) and flow rate of 1.5 ml/min (high-performance liquid chromatograph, Agilent Technologies, 1200 series, USA) [2,12]. Additionally, the presence of amino acids and essential minerals in PPH were analyzed by HPLC and Inductively Coupled Plasma Optical Emission spectroscopy (ICP-OES), respectively as described by standard procedures.…”

“…Briefly, 500 µl of cell-free supernatant was added to 500 µl of the color reagent. These solutions were heated in boiling water for 10 min and immediately transferred on ice, and the absorbance was measured at 540 nm when the calibration curve was glucose at 0 to 1.0 g/l [2,12].…”

“…Among these, pineapple peel residue has become an attractive low-cost substrate owing to its high sugar content, e.g. sucrose, glucose, fructose, and other carbohydrates, which provide the necessary nutrients for bacterial growth and PHA biosynthesis [1,2,12]. Valorization of this lignocellulosic waste could be a sustainable and eco-friendly solution, in addition to non-competition with food values [5,13,14].…”

Recent Updates on the Conversion of Pineapple Waste (Ananas comosus) to Value-Added Products, Future Perspectives and Challenges

“…Few studies have reported the potential use of pineapple waste in producing PHAs. Earlier, Suwannasing et al [15] reported that pineapple cannery wastes were used to produce PHAs. The produced PHAs were analysed using Fourier Transform Infra-Red (FTIR), and the results showed that the PHAs were present in the form of polyhydroxybutyrate (PHB).…”

“…Considering its characteristics, composition and health benefits, pineapple waste contains proteolytic enzyme for bromelain production [11] and phenolic compound which is a good antioxidant source including antimicrobial and anticancer properties [12]. Pectin, lignin, cellulose and their derivatives from pineapple waste are the major components needed in producing nanocellulose and green packaging [13][14][15]. Since pineapple waste is a good carbon source, organic acid [16], wine [17], vinegar [18], dye adsorbent [19], biofuel [20][21][22] and biogas [23][24][25] can be produced from it.…”