Potential viral vectors for the stimulation of mucosal antibody responses against enteric viral antigens in pigs

Abstract

Four viruses were compared for their ability to induce an intestinal antibody response in piglets. Antibodies were not detected in response to oral vaccination with either fowlpox virus or a baculovirus (BV). Simultaneous oral dosing and parenteral inoculation with high concentrations of ~v in an oil emulsion adjuvant induced high levels of circulating virus neutralising (VN) antibodies, and also low levels of intestinal antibodies when booster doses of virus were given. In response to oral vaccination with swinepox virus (sPy), low levels of circulating and intestinal VN antibodies, and higher titres of antibodies reactive in an enzyme immunoassay, including intestinal antibodies of the IgA class, were detected. Oral vaccination with porcine adenovirus type 3 (PAV-3) stimulated both circulating and intestinal VN antibodies, and IgA antibodies were demonstrated in the intestinal contents.It was concluded that sPv and PAV-3 might be suitable vectors for the expression of genes en: coding the protective antigens of porcine enteric viruses.

An adenovirus recombinant expressing the spike glycoprotein of porcine respiratory coronavirus is immunogenic in swine

“…Therefore, they may be particularly well suited as recombinant live vectors (Tuboly et al, 1993) for antigen delivery at the respiratory mucosal lymphoid tissues. Human adenovirus type 5 (Ad5), the serotype most commonly used in current work, can accept inserts of up to 4 kbp of foreign DNA following a partial deletion of the non-essential early transcription region 3 (E3) from the genome (Graham & Prevec, 1991).…”

Effect of different levels of mannan‐oligosaccharide supplementation on some immunological variables in weaned piglets

“…All piglets were weighed and slaughtered at week 10. Digesta from three different parts of the small intestine were collected as described by Tuboly et al. (1993) and tested for the presence of TGEV and secretory (s)IgA antibodies.…”

Circumventing Antivector Immunity: Potential Use of Nonhuman Adenoviral Vectors

“…The idea of using PAdV (genus Mastadenovirus, species Porcine A, B, C) vectors was published over 20 years ago (Tuboly et al, 1993). A few years later it was discovered that the virus can enter but is unable to replicate in dog, sheep, bovine, and human cells (Reddy et al, 1999a).…”