Potential virulence and antimicrobial susceptibility of Aeromonas popoffii recovered from freshwater and seawater

Soler, L

doi:10.1016/s0928-8244(01)00303-0

Cited by 26 publications

(34 citation statements)

References 28 publications

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“…All presumptive Aeromonas isolates were first screened using Aeromonas-genus-specific primers targeting the glycerophospholipid cholesterol acyltransferase gene (gcat) using the reaction and cycling conditions described by Soler et al (2002); they were then identified at the species level based on the sequencing analysis of 2 housekeeping genes, rpoD and gyrB. An approximately 1100 bp fragment of gyrB and a 820 bp fragment of rpoD were amplified from the template DNA, purified using the FastGene Gel/PCR extraction kit (Nippon Genetics) and sequenced using the BigDye Terminator V3.1 Cycle Sequencing Kit on the 3130xl Genetic Analyzer (Applied Biosystems).…”

Section: Dna Extraction and Molecular Identificationmentioning

confidence: 99%

“…All the isolates were screened by PCR for the presence of 7 genomic markers potentially linked to virulence -aerolysin (aerA), cytotoxic enterotoxin (act), the heat-stable and heat-labile cytotonic enterotoxins (ast and alt, respectively), serine protease (ser), DNase (exu) and flagellin (fla) -using the primers and conditions described by Nawaz et al (2010) and Soler et al (2002). The sequence of each primer used to amplify the target genes, the expected size of the PCR products and their references are listed in Table S3 in the Supplement.…”

Section: Detection Of Virulence-related Genes By Pcrmentioning

confidence: 99%

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Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia

Jagoda¹,

Wijewardana²,

Arulkanthan³

et al. 2014

Dis. Aquat. Org.

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Section: Dna Extraction and Molecular Identificationmentioning

confidence: 99%

Section: Detection Of Virulence-related Genes By Pcrmentioning

confidence: 99%

Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia

Jagoda¹,

Wijewardana²,

Arulkanthan³

et al. 2014

Dis. Aquat. Org.

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“…Although most 16S rRNA gene sequence-derived relationships have correlated well with the DNA homology groups (HG), difficulties have been encountered in splitting closely related species (Martínez-Murcia, 1999;Martínez-Murcia et al, 2005). DNA probes and restriction fragment length polymorphism (RFLP) profiles designed from 16S rRNA gene diagnostic regions have served to identify Aeromonas at the species level (Ash et al, 1993a, b;Borrell et al, 1997;Dorsch et al, 1994;Figueras et al, 2000;Khan & Cerniglia, 1997;Lee et al, 2002;Soler et al, 2002). Recent sequencing analyses based on the gene sequences of gyrB (encoding the B subunit of DNA gyrase, a type II DNA topoisomerase) and rpoD (encoding the s 70 factor, one of the sigma factors that confers promoter-specific transcription initiation on RNA polymerase) have demonstrated that they are excellent molecular markers for phylogenetic inference in the genus Aeromonas (Soler et al, 2004;Yáñez et al, 2003) and these chronometers seem to be well synchronized.…”

mentioning

confidence: 99%

Updated phylogeny of the genus Aeromonas

Saavedra

Figueras

Martínez‐Murcia

2006

International Journal of Systematic and Evolutionary Microbiology

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Recent phylogenetic studies of the genus Aeromonas based on gyrB and rpoD gene sequences have improved the phylogeny based on 16S rRNA gene sequences first published in 1992, particularly in the ability to split closely related species. These studies did not include the recently described species Aeromonas simiae and Aeromonas molluscorum and only a single strain of Aeromonas culicicola was available for analysis at that time. In the present work, these Aeromonas species and newly isolated strains of A. culicicola were examined. Sequence analysis indicates that A. simiae and A. molluscorum belong to non-described phylogenetic lines of descent within this genus, which supports the original description of both species. The most closely related species are Aeromonas schubertii and Aeromonas encheleia, respectively, which is consistent with 16S rRNA gene sequencing results. However, while the five strains of A. molluscorum showed nucleotide differences in their gyrB and rpoD gene sequences, the only two known A. simiae strains exhibited identical gene sequences, suggesting that they are isolates of the same strain. On the basis of the rpoD gene sequence phylogeny, A. culicicola strains from the original description and new isolates from drinking water and ornamental fish clustered within the species Aeromonas veronii, suggesting inconsistencies with previous results. Other strains with previously controversial taxonomy and new isolates from other studies were included in this study in order to clarify their phylogenetic affiliation at the species level.

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“…However, this bacterium is able to produce some virulence factors, conferring a pathogenic role. These genes were present in all A. popoffii strains studied by Soler et al (11,12). For the isolate described here, we demonstrated the presence of genes coding for aerolysin/hemolysin and serine protease.…”

mentioning

confidence: 82%

Aeromonas popoffii Urinary Tract Infection

Hua¹,

Bollet²,

Tercian³

et al. 2004

J Clin Microbiol

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Aeromonas popoffii is a recently described species isolated mainly from freshwater. An isolate of Aeromonas popoffii was found to be responsible for a urinary tract infection in a 13-year-old boy suffering from spina bifida with enterocystoplasty. This is the first reported case of human infection attributed to this species. CASE REPORTA 13-year-old boy was hospitalized in April 2003 for replacement of a urethral catheter. He suffered from congenital spina bifida and myelomeningocele complicated by a neurogenic bladder treated by anticholinergic drugs and intermittent bladder catheterization for 5 years. Imaging explorations did not reveal vesico-ureteral reflux. In 2001, the patient unsuccessfully underwent two transurethral injections of macroplastic substance on the vesical neck in order to palliate increasing urinary incontinence. He underwent an enterocystoplasty, using the sigmoid colon with surgical correction of the vesical neck (Young Dees type) and bilateral ureteral reimplantation with an antireflux valve (Cohen type) in April 2003. On the 10th postoperatory day, he returned home and was again treated by intermittent bladder catheterization (about four times a day). On day 15, the patient presented a fever of 38°C associated with left flank pain, and he was hospitalized. The urine analysis showed 10 5 polymorphonuclear leukocytes and 10 6 bacteria per ml. Blood leukocytes rose from 5,700 to 8,500/ l, platelet count rose from 321,000 to 488,000/l, and the sedimentation rate was 28 mm for the first hour. Preventive antibiotic treatment had been continued postoperatively with intravenously administered ceftriaxone (2 g/day) and metronidazole (1,500 mg/day) in the hospital, which was changed to amoxiclav given orally (1,500 mg/day) when he went home.The microbiological result confirmed a urinary tract infection due to Aeromonas sp., with intermediate susceptibility to amoxicillin and amoxicillin-clavulanate and susceptibility to ceftriaxone, ciprofloxacin, gentamicin, and cotrimoxazole. Treatment was changed to orally administered cotrimoxazole (800/160 mg twice per day), with a favorable clinical evolution later.Urine was cultured aerobically on Trypticase soy agar (bioMérieux, Marcy l'Etoile, France) at 37°C. After 48 h, 3-mm whitish colonies with irregular edges were observed. Gram staining revealed gram-negative, straight motile rods. Catalase and oxidase were positive, with resistance to vibriostatic agent O/129. The API-20 NE (bioMérieux) profile was 7 477 745, corresponding to a low level of discrimination between Aeromonas hydrophila or Aeromonas caviae (percentage of identification, 91.5%; T index, 0.76) and Vibrio parahaemolyticus (8.3% identical; T index, 0.58) (profile book, 6th ed., 1997).The antibiotic susceptibility profile was studied on sheep blood Mueller-Hinton agar plates by the disk diffusion method according to NCCLS recommendations (10). The MICs were 2 g/ml for amoxicillin, 0.25 g/ml for ceftriaxone, 4 g/ml for imipenem, 0.5 g/ml for amikacin, 0.75 g/ml for ciprofloxacin, and 0.25 ...

show abstract

Potential virulence and antimicrobial susceptibility of Aeromonas popoffii recovered from freshwater and seawater

Cited by 26 publications

References 28 publications

Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia

Characterization and antimicrobial susceptibility of motile aeromonads isolated from freshwater ornamental fish showing signs of septicaemia

Updated phylogeny of the genus Aeromonas

Aeromonas popoffii Urinary Tract Infection

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