Potential Wound Healing Activities of Galla Rhois in Human Fibroblasts and Keratinocytes

Park, Hyo-Hyun; Park, Na-Young; Kim, Sun-Gun; Jeong, Kyu-Tae; Lee, Eu Jin; Lee, Eunkyung

doi:10.1142/s0192415x15500925

Cited by 12 publications

(8 citation statements)

References 18 publications

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“…Normally, for the damage of the external environment, skin can protect the integrity and function of internal organs very effectively (22,32). Therefore, in the process of resisting environmental stimuli, the skin will be damaged to varying degrees.…”

Section: Discussionmentioning

confidence: 99%

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Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

et al. 2020

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The skin protects body from environmental damage. Skin wounds lead to microbial infection and harmful agent injury. Thus, wound repair is crucial for the recovery of the normal function of skin tissue. The present study investigated the promoting effects of spirulina protein (SPcP) in mice on skin wound repair and also aimed to elucidate the potential underlying mechanisms. The results revealed that SPcP promoted the skin wound repair in a mouse model of full-thickness excisional wounds. SPcP induced an increase in the expression level of α-smooth muscle actin (α-SMA). The activities of superoxide dismutase (SOd) and catalase (cAT) were enhanced by SPcP treatment in the granulation tissue. In addition, SPcP decreased the level of malondialdehyde (MdA) in the granulation tissue. Western blot analysis revealed that SPcP enhanced the phosphorylation and activation of protein kinase B (Akt) and extracellular signal-regulated kinase (ERK). Moreover, the expression level of transforming growth factor β1 (TGF-β1) was increased in the SPcP-treated groups. The phosphorylation level of Smad2 was also increased by treatment of SPcP. Furthermore, SPcP promoted the expression of collagen in the granulation tissue. Taken together, these findings indicate that SPCP exerts a promoting effect on skin wound repair. The Akt, ERK and TGF-β1 signaling pathways are involved in this process.

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Section: Discussionmentioning

confidence: 99%

“…Appropriate levels of ROS are beneficial for wound repair. However, excessive ROS production leads to oxidative stress, and inhibits the proliferation and migration of cells in wound repair (21,22). In addition, high levels of oxidative stress prolong the inflammatory process, induce tissue damage and results in the delay of wound repair.…”

Section: Introductionmentioning

confidence: 99%

Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

et al. 2020

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“…In general, skin is regarded as a barrier to protect the inner organs from environmental damage, which makes it extremely vulnerable to different types of lesions [30]. Skin wounds can cause the inner organ to become ill with poor nutrition and even death [31].…”

Section: Discussionmentioning

confidence: 99%

Wound Healing Potential of Spirulina Protein on CCD-986sk Cells

et al. 2019

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Wound healing is a dynamic and complex process. The proliferation and migration of dermal fibroblasts are crucial for wound healing. Recent studies have indicated that the extracts from Spirulina platensis have a positive potential for wound healing. However, its underlying mechanism is not fully understood. Our previous study showed that spirulina crude protein (SPCP) promoted the viability of human dermal fibroblast cell line (CCD-986sk cells). In this study, we further investigated the wound healing effect and corresponding mechanisms of SPCP on CCD-986sk cells. Bromodeoxyuridine (BrdU) assay showed that SPCP promoted the proliferation of CCD-986sk cells. The wound healing assay showed that SPCP promoted the migration of CCD-986sk cells. Furthermore, cell cycle analysis demonstrated that SPCP promoted CCD-986sk cells to enter S and G2/M phases from G0/G1 phase. Western blot results showed that SPCP significantly upregulated the expression of cyclin D1, cyclin E, cyclin-dependent kinase 2 (Cdk2), cyclin-dependent kinase 4 (Cdk4), and cyclin-dependent kinase 6 (Cdk6), as well as inhibited the expression of CDK inhibitors p21 and p27 in CCD-986sk cells. In the meanwhile, SPCP promoted the phosphorylation and activation of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt). However, the phosphorylation of Akt was significantly blocked by PI3K inhibitor (LY294002), which in turn reduced the SPCP-induced proliferation and migration of CCD-986sk cells. Therefore, the results presenting in this study suggested that SPCP can promote the proliferation and migration of CCD-986sk cells; the PI3K/Akt signaling pathway play a positive and important role in these processes.

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“…Moreover, HSF also played an important role in wound healing. 26,27 We therefore experimentally tested whether exosomal miR-19b could have an effect on HSF. We first confirmed that exosomes could be taken up by HSF, and we observed a significant uptake of exosomes by HSF after 48 hours of incubation with exosomes ( Figure 6A).…”

Section: Exosomal Mir-19b Contributes To the Proliferation And Migratmentioning

confidence: 99%

<p>Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-β Signaling Axis</p>

Cao¹,

Chen²,

Zhang³

et al. 2020

CCID

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Introduction Human adipose-derived mesenchymal stem cells (ADMSCs) with their secretory factors are able to induce collagen synthesis and fibroblast migration in the wound healing process. This study is launched to figure out the effect of human ADMSCs-derived exosomes on skin wound healing. Methods ADMSCs were extracted and ADMSCs-derived exosomes were identified. Skin damage models were established by treating HaCaT cells and human skin fibroblasts with H 2 O 2 . Next, the roles of ADMSCs and their derived exosomes were investigated. The exosomal miRNA then was analyzed, and the function of miRNA on the H 2 O 2 -induced cells was studied by miRNA suppression. Bioinformatics analysis, luciferase activity and RIP assays were implemented to find the target genes ofthe miRNA and the modulated pathways. A mouse skin damage model was induced to elucidate the effects of exosomes in vivo by injecting exosomes. Results H 2 O 2 treatment significantly reduced the viability of HaCaT cells and increased their apoptosis rate. Co-culture with ADMSCs or their derived exosomes could improve the cell damage caused by H 2 O 2 . Meanwhile, H 2 O 2 treatment promoted the internalization of exosomes. ADMSCs and their derived exosomes significantly increased miR-19b expression in the recipient cells, while inhibiting miR-19b resulted in a reduction in the therapeutic effect of ADMSCs-derived exosomes. Besides, miR-19b regulated the TGF-β pathway by targeting CCL1. The therapeutic effect of exosomes was further confirmed by a mouse model of skin damage. Conclusion Our study indicates that exosomal miR-19b derived from ADMSCs regulates the TGF-β pathway by targeting CCL1, thereby promoting the healing of skin wounds.

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Potential Wound Healing Activities of Galla Rhois in Human Fibroblasts and Keratinocytes

Cited by 12 publications

References 18 publications

Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

Spirulina protein promotes skin wound repair in a mouse model of full-thickness dermal excisional wound

Wound Healing Potential of Spirulina Protein on CCD-986sk Cells

<p>Human Adipose-Derived Mesenchymal Stem Cells-Derived Exosomal microRNA-19b Promotes the Healing of Skin Wounds Through Modulation of the CCL1/TGF-β Signaling Axis</p>

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