Potential Yeast from Indonesian Wild Forest Honey Showing Ability to Produce Lipase for Lipid Transesterification

Abstract

Biodiesel is produced through the transesterification process in the presence of alcohol and a catalyst that catalyzes the conversion of triglycerides to esters and glycerol compounds. A more optimal product conversion can be achieved using enzymes, such as lipase. Lipase is reported to be produced in osmophilic yeasts due to the low water content in their natural habitats. Wild forest honey is one of the osmophilic natural habitats in Indonesia. However, lipase-producing yeast has not been reported in the Indonesian honey. In this study, we screened the lipase-producing yeasts isolated from wild forest honey collected from Central Sulawesi. The production profile and activity of lipase were determined at different pH values and temperatures. One promising yeast was isolated from the honey, which was identified as Zygosaccharomyces mellis SG 1.2 based on ITS sequence. The maximum lipase production (24.56 ± 1.30 U/mg biomass) was achieved by culturing the strain in a medium containing 2% olive oil as a carbon source at pH 7 and 30℃ for 40 h. The optimum pH and temperature for lipase activity were 6 and 55℃, respectively. The enzyme maintained 80% of its activity upon incubation at 25℃ for 4 h. However, the enzyme activity decreased by more than 50% upon incubation at 35 and 40℃ for 2 h. This is the first study to report the lipase producing capability of Z. mellis. Further studies are needed to optimize the enzyme production.

Optimization of Medium for Lipase Production from Zygosaccharomyces mellis SG1.2 Using Statistical Experiment Design

“…Validation was performed by growing the strain Z. mellis SG 1.2 into production medium in a previous study by Palilu et al [11]…”

“…Microorganism lipases have previously been studied, such as Yarrowia lypolitica which has productivity of 0.758 U/mg, Aspergillus ibercus 0.127 ± 0.017 U/ mg and Penicillium restrictum with productivity of 0.0123 U/mg [8−10]. Zygosaccharomyces mellis SG 1.2 isolated from forest honey was able to produce lipase and had a lipase productivity of 24.56 U/mg of biomass [11]. Z. mellis SG 1.2 is a promising new source of lipase and its productivity needs to be increased by optimization.…”

Analysis of the Diversity of Yeast Communities in Xinjiang Honey

“…But so far, whether the yeast in honey is detrimental to health is still unknown (Devi et al 2021). Some people have reported that yeast that produces erythritol from honey has been found (Deng et al 2013) ethanol-producing yeast (Joseph et al 2018), lipaseproducing yeast (Palilu et al 2019), but yeast can also affect the quality of honey (Hajimohammadi et al 2017). Therefore, we are quite interested in the biodiversity of yeast in honey.…”

“…Rering Caitlin C et al found that pollen and yeast may work together to alter the chemical composition of nectar, thereby affecting honey quality (Rering et al 2021). Echeverrigaray Sergio et al isolated yeast from 17 stingless bee honey samples in southern Brazil and found that some yeasts were only identi ed in honey samples from speci c bee species, indicating that these yeasts are closely related to insects (Echeverrigaray et al 2021), and the isolated yeast species exhibited high osmotic pressure (Palilu et al 2019)and low sugar assimilation (Park et al 1996)capacity. Osmophilic yeasts such as Yong Kun Park isolated from honey and pollen not only convert sucrose to polymers but also to fructooligosaccharides in good yields.…”

“…It has been reported that osmophilic yeast produce lipase due to their low water content in their natural habitat. Palilu Prayolga Toban (Palilu et al 2019)Lipase-producing yeast isolated from wild forest honey in Sulawesi. Xinjiang is a traditional beekeeping province with an extensive mass beekeeping base, and it has a typical continental climate with large diurnal temperature differences(ZHANG et al 2020) providing unique conditions for the formation and evolution of species (Zhu et al 2021).…”