Potentiality of an organometallic labelled streptavidin—biotin system in metalloimmunoassay

Remy, Isabelle; Brossier, Pierre; Lavastre, I.; Besançon, J.; Moı̈se, Claude

doi:10.1016/0731-7085(91)80031-4

Cited by 9 publications

(2 citation statements)

References 5 publications

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“…The detection is based on the intrinsic elemental composition of labeling reagents (atomic spectrometry), thus largely alleviate the worry about their synthesis and stability. However, due to either the lack of multiplex potential or low sensitivity, the detection by atomic spectrometry including atomic absorption spectrometry (AAS), atomic fluorescence spectrometry (AFS), and atomic emission spectrometry (AES) are useful but not attractive (Mariet et al, ; Remy et al, ; Wang et al, ; Wu et al, ; Liang et al, ; Liu et al, ; Tung et al, ). Only after the distinct merits of multiplex potential and high sensitivity were brought by ICPMS, the combination of atomic spectrometry and element‐tagged immunoassay opened up a new avenue for quantitative bioanalysis.…”

Section: Icpms‐based Quantitative Immunoassaymentioning

confidence: 99%

Inductively coupled plasma mass spectrometry‐based immunoassay: A review

Liu

Yang

et al. 2013

Mass Spectrometry Reviews

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The last 10 years witnessed the emerging and growing up of inductively coupled plasma mass spectrometry (ICPMS)-based immunoassay. Its high sensitivity and multiplex potential have made ICPMS a revolutionary technique for bioanalyte quantification after element-tagged immunoassay. This review focuses on the major developments and the applications of ICPMS-based immunoassay, with emphasis on methodological innovations. The ICPMS-based immunoassay with elemental tags of metal ions, nanoparticles, and metal containing polymers was discussed in detail. The recent development of multiplex assay, mass cytometry, suspension array, and surface analysis demonstrated the versatility and great potential of this technique. ICPMS-based immunoassay has become one of the key methods in bioanalysis.

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Section: Icpms‐based Quantitative Immunoassaymentioning

confidence: 99%

Inductively coupled plasma mass spectrometry‐based immunoassay: A review

Liu

Yang

et al. 2013

Mass Spectrometry Reviews

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“…CMT and its derivatives are stable, finding use as an anti-knocking additive in gasoline. 22 CMT has been investigated in the form of bioconjugates with biotin [23][24][25] and with cell penetrating peptides as a means of introducing cytotoxic carbon monoxide to cancer cells. We are investigating the suitability of a suite of metal carbonyl complexes for use as biophysical probes, including cymantrene due to its relatively wide array of proven applications.…”

Section: Introductionmentioning

confidence: 99%

Ultrafast 2DIR probe of a host-guest inclusion complex: Structural and dynamical constraints of nanoconfinement

Osborne

King

Dunbar

et al. 2013

The Journal of Chemical Physics

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Two-dimensional infrared (2DIR) spectroscopy is used to study the influence of nanoconfinement on the spectral diffusion dynamics of cyclopentadienyl manganese tricarbonyl (CpMn(CO)3, CMT) free in solution and confined in the cavity of β-cyclodextrin. Contrary to the reorientation correlation function of the solvent molecules, determined through molecular dynamics simulations, measurements in three different solvents indicate that CMT confined in β-cyclodextrin undergoes spectral diffusion that is faster than free CMT. To account for this discrepancy, we propose that spectral diffusion time scales contain a dynamical contribution that is dependent on the effective size of the conformational space presented by the solvation environment. This solvation state space size is related to the number of participating solvent molecules, which in turn is proportional to the solvent accessible surface area (SASA). We test the role of the number of participating solvent molecules using a simple Gaussian-Markov simulation and find that an increase in the number of participating solvent molecules indeed slows the time required to search the available conformational space. Finally, we test this dependence by comparing the spectral diffusion of a previously studied manganese carbonyl, dimanganese decacarbonyl (Mn2(CO)10, DMDC), to CMT and find that DMDC, which has a larger SASA, exhibits slower spectral diffusion. The experimental observations and the supporting simplistic solvation model suggest that vibrational probe molecules, such as CMT, might be able to function as sensors of conformational entropy.

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