Potentials of leaves of Aspilia africana (Compositae) in wound care: an experimental evaluation

Okoli, C. O.; Akah, P. A.; Okoli, AS

doi:10.1186/1472-6882-7-24

Cited by 84 publications

(76 citation statements)

References 14 publications

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“…More so, saponnins have been reported to have precipitating and coagulative properties. The result of this study coupled with increased mean platelet volume (MPV) in the aqueous extract and results from previous studies [12][13][14] suggested that the haemostatic property of Chromolaena odorata is mostly from the platelet activity/quality rather than quantity or clotting factors. This was further corroborated by the insignificant difference in the PT and PTTK of the test and control groups ruling out significant contributions from clotting factors.…”

Section: Discussionmentioning

confidence: 75%

Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats

et al. 2016

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This study was designed to investigate the effects of aqueous, ethanol and crude extracts of Chromolaena odorata leaf on haemostatic mechanism of wistar rats and its possible in vitro use in coagulation study. Fifty wistar rats of both sexes weighing between 140-180 g were sorted into 10 groups each fed via oral gavage once daily for 21 days. Sample collection was done by cardiac puncture. Bleeding and clotting times were performed using Duke's and Ivy's methods, respectively. The prothrombin time was performed using the Quick's one stage method, while the partial thromboplastin time using kaolin was done using Macpherson and Hardity method using Giess diagnosis reagent. Significant results were observed in the bleeding and clotting times of the three extracts in a dose-dependent manner. The 300-mg/kg ethanol extract decreased the bleeding time more than the other two extracts. Only the ethanol 150 and 300 mg/mL showed in vitro activity. The study showed the in vivo haemostatic properties of Chromolaena odorata leaf extracts and its possible use in in vitro coagulation study.

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Section: Discussionmentioning

confidence: 75%

Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats

et al. 2016

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“…Crude extracts of plants have been found very useful in ethnomedical practice as part of the process for the treatment of diseases (IWU 1982). In this study, it was shown from the phytochemical studies that there is presence of typical plant constituents (Okoli 2007) such as carbohydrate, calcium, alkaloids, reducing sugar, flavenois, saponins and glycoside, fats and oils, while other constituents such as tanins, resins, terpenoids, acidic compounds, steroids were absent. The acute toxicity study showed that the Canabis sativa extract was non-toxic as shown by the LD 50 (fig 1) of 3000mg/kg.…”

Section: Discussionmentioning

confidence: 99%

Efficacious Effect of Cannabis Sativa (Indian Hemp) on Cutaneous Wound Healing and its Haemostatic and Chemical Profile in Adult Male Albino Wistar Rat

Oguwike¹,

Chianumba²,

Ukekwe³

et al. 2016

GJMS

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Plants and plant extracts have been found very useful for purposes of treatment of disease among other benefits.Cannabis sativa is one of such plants whose effect on wound healing, haemostatic and biochemical profile was studied using albino wistar rats. Canabis sativa is an illicit drug with proven anti-inflammatory, analgesic and anti-diarrhea activities. This present study is aimed at ascertaining the impact of prolonged administration of cannabis sativa on haemostatic mechanism, cutaneous wound healing and biochemical profile of albino wistar rats. Forty (40) albino wistar rats weighing 180-200g were acclimatized in the animal house for 7 days. After this, they were divided into five groups respectively. Group A was the control male rats (6), Group B was the female control rats (6), Group C (10) was the male test rats and Group D (10) was the femaletest rats and Group E (8) for incision. Group C and D male rats were fed for 30 days with aqueous extract of Canabis sativa before 5.0ml of blood sample was collected from the animals for cardiac puncture and placed in haemogram bottles (EDTA and sodium citrate) for haemostatic analysis and in plain bottles for liver function tests while Group E rats were those with incision made in their skin that received topical treatment of aqueous extract of cannabis sativa and injection for 5days.Results from the study showed that the extract significantly increased the total and conjugated bilirubin, (P<0.05), liver enzymes; Alkaline Phosphatase (ALP), Aspartate Transaminase (AST) and Alanine Transaminase (ALT), Haemoglobin concentrations, Packed cell volume, platelet count and white blood cell count. The bleeding and clotting times,ProthrombintimeandPartialthromboplastin times were shortened compared to their corresponding controls. The incised wounds haled within shorter days than those in control rats. The alterations in the results are manifestation of mild hepatorenal toxicity and anti-hypercholestrolemic effect. These liver enzymes are highly affected by cannabis sativa in females than in males.

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“…Crude extracts of plants have been found very useful in ethnomedical practice as part of the process for the treatment of diseases (Iwu, 1982). In this study, it was shown from the phytochemical studies that there is presence of typical plant constituents (Okoli, 2007) such as calcium, alkaloids, carbohydrates, tannins, saponins, fats, reducing sugar, while other constituents such as resins, terpenoids, acidic compounds, steroids, oil, glycosides were absent. The calcium and alkaloids (protein precipitates) contained in this plant extract are known to be involved in the precipitation of coagulation factors (Taofeeq et al, 2008) which promotes clotting in rats.…”

Section: Discussionmentioning

confidence: 99%

Effect of Pawpaw (Carica papaya) Latex on the Haemostatic Mechanism and Biochemical Profile of Male Albino Wistar Rats

Oguwike¹,

Nwafia²,

Nwozor³

et al. 2017

GJMS

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Extracts of roots, stem, bark, leaves and sap of some medicinal plants have been known to have effect against the most dreaded pathogenic organism like the bacteria, Fungi, viruses (Russel et al, 1997). Soldiers need training, men and women do engage in exercises, children play in the field, most times people are not allowed to partake in their trainings, exercises or play due to bleeding from minor injuries which could be arrested using simple remedies from plants that could be found around the bush. Hence the effect of pawpaw (Carica papaya) latex on the haemostatic mechanism and biochemical profile of male albino wistar rats was studied to show the ability of Carica papaya latex to enhance haemostatic mechanism and stop bleeding.Forty (40) male albino rats weighing 200-250g were selected for the study. Group 1 (10 rats) is the control group. Group 2 (10 rats) received topical application of the latex after wound laceration, Group 3 (10 rats) was fed orally (Enteral) with the latex for 5 days, while Group 4 (10 rats) was injected (Parenteral) with the latex (0.5ml) for 5 days. Thereafter, blood samples were collected from Groups 1, 3 and 4 for analysis such as Full blood count, Platelet count, Prothrombin time test, Partial thromboplastin time test, bleeding time, clotting time, biochemical test such as serum total bilirubin, conjugated bilirubin, alkaline phosphatise, aspartate transaminase and alanin transaminase.Calcium and alkaloids (protein precipitates) was discovered present in the latex of Carica papaya. They are known to be involved in the precipitation of coagulation factors (Tafeeq, et al 2008) which promotes clotting in rats. The latex of Carica papaya reduced haemoglobin concentration, packed cell volume and white blood cell count significantly (p<0.05) but the platelet count was very high (p<0.05). It also increased the total bilirubin, conjugated bilirubin and the liver enzymes but shortened bleeding and clotting times in vitro. It could be deduced that the presence of calcium and papain helped to reduce the bleeding and clotting times.

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Potentials of leaves of Aspilia africana (Compositae) in wound care: an experimental evaluation

Cited by 84 publications

References 14 publications

Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats

Haemostatic property of Chromolaena odorata leaf extracts: in vitro and in vivo evaluation in wistar rats

Efficacious Effect of Cannabis Sativa (Indian Hemp) on Cutaneous Wound Healing and its Haemostatic and Chemical Profile in Adult Male Albino Wistar Rat

Effect of Pawpaw (Carica papaya) Latex on the Haemostatic Mechanism and Biochemical Profile of Male Albino Wistar Rats

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