Potentiation of Bacillus thuringiensis insecticidal activity by serine protease inhibitors

MacIntosh, Susan; Kishore, Ganesh M.; Perlak, Frederick J.; Marrone, Pamela G.; Stone, Terry B.; Sims, Steven R.; Fuchs, Roy L.

doi:10.1021/jf00094a051

Cited by 131 publications

(79 citation statements)

References 22 publications

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“…It is also possible that boiling the soybean flour in water solubilises the tyrpsin inhibitors. KSTI and other trypsin inhibitors are known to have synergistic effects with delta endotoxins of Bacillus thuringiensis against H. armigera and other insects (Manduca sextai, Heliothis virescens, Trichoplusia ni, Helicoverpa zea) and Leptinotarsa decemlineata (Colorado potato beetle) ( Macintosh et al, 1990;Shao et al, 1998;Zhang et al, 2000, Zhu et al, 2007Christtellier et al, 1992;Hubert et al, 2008). It was suggested that the mechanism of synergism might be prevention of activation of the toxin by gut serine proteases due to KSTI action (Zhu et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…KSTI and other potent trypsin inhibitors are also known to have synergistic effects with delta endotoxins of Bacillus thuringiensis. This has been shown in Lepidoptera (H. armigera, Manduca sexta, Heliothis virescens, Trichoplusia ni and H. zea), as well as Leptinotarsa decemlineata (Colorado potato beetle) (Macintosh et al, 1990;Shao et al, 1998;Zhang et al, 2000, Zhu et al, 2007Christtellier et al, 1992;Hubert et al, 2008). A recent study has shown that raw soybean diets can prevent the detection of non-specific esterase mediated resistance to Bacillus thuringensies toxins in H. armigera (Gunning & Moores, 2010).…”

Section: Introductionmentioning

confidence: 87%

“…Leguminous seeds, such as those from soybean, are protected against herbivores, including insects, by anti-nutritional defence proteins-trypsin inhibitors, which are found in the seeds and raw flour made from the seeds. Levels of these plant protease inhibitors are high in legume seeds, comprising 1-5% of total protein (Macintosh et al, 1990;Sastry et al, 1987) Trypsin inhibitors of the Kunitz type (Kunnitz, 1945a;Kunnitz, 1945b) are single chain polypeptides (~ 20 kDa) that act on target serine proteases in the gut forming a 1:1 complex. Perhaps the best known of these inhibitors is Kunitz soybean trypsin inhibitor (KSTI).…”

Section: Introductionmentioning

confidence: 99%

“…Midgut serine protease activity has been found in a wide variety of Lepidopteran pests, including H. armigera, where trypsin is a major protease in the H. armigera midgut (Macintosh et al, 1990). In H. armigera, KSTI acts against gut serine proteases and are larval growth retardants and may cause death through the prevention of protein digestion (Johnston et al, 1993;Wang et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Dietary Soybean Trypsin Inhibitors on Detection of Resistance to Pyrethroid and Spinosad Insecticides in Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Gunning¹,

Moores²

2011

Soybean and Health

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Dietary Soybean Trypsin Inhibitors on Detection of Resistance to Pyrethroid and Spinosad Insecticides in Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Gunning¹,

Moores²

2011

Soybean and Health

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“…Activity of Bt in transgenic plants can be enhanced by serine protease inhibitors (MacIntosh et al 1990). Activity of Bt can also be increased in combination with tannic acid (Gibson et al 1995).…”

Section: Gene Pyramidingmentioning

confidence: 99%

Prospects for using transgenic resistance to insects in crop improvement

Sharma¹,

Sharma²,

Seetharama³

et al. 2000

Electron. J. Biotechnol.

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Integrated pest management has historically placed great hopes on host plant resistance. However, conventional host-plant resistance to insects involves quantitative traits at several loci. As a result, the progress has been slow and difficult to achieve. With the advent of genetic transformation techniques, it has become possible to clone and insert genes into the crop plants to confer resistance to insect pests. Resistance to insects has been demonstrated in transgenic plants expressing genes for δ δ-endotoxins from Bacillus thuringiensis (Bt), protease inhibitors, enzymes and plant lectins. Most of the plant derived genes produce chronic rather than toxic effects and some insect pests are not sensitive to some of these factors. The potential of plant derived genes can be realised by deploying them in combination with host plant resistance and exotic genes. Genes conferring resistance to insects have been inserted into crop plants such as maize, cotton, potato, tobacco, potatoes, rice, broccoli, lettuce, walnuts, apples, alfalfa and soybean. Genetically transformed crops with Bt genes have been deployed for cultivation in USA, China and Australia. However, very little has been done to use this technology for improving crop production in the harsh environments of the tropics, where the need for increasing food production is most urgent. International agricultural research centres, advanced research institutes and the seed sector should make an effort to use these new tools for increasing food *Corresponding author production in poorer regions of the world. There is an urgent need to develop a scientifically sound strategy to deploy exotic and plant derived genes for minimising the extent of losses caused by insect pests. Equally important is the need for following the biosafety regulations, more responsible public debate, social attitude and better presentation of the benefits for a rational deployment of the genetically transformed plants.

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Identifying proteins with insecticidal activity: use of encoding genes to produce insect-resistant transgenic crops

Gatehouse

1998

Pestic. Sci.

169

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: Crops resistant to insect attack o †er an alternative strategy of pest control to a total reliance upon chemical pesticides. Transgenic plant technology can be a useful tool in producing resistant crops, by introducing novel resistance genes into a plant species. This technology is seen very much as forming an integral component of a crop management programme.Several di †erent classes of plant proteins have been shown to be insecticidal towards a range of economically important insect pests from di †erent orders ; in some cases a role in the defence of speciÐc plant species against phytophagous insects has been demonstrated. Genes encoding insecticidal proteins have been isolated from various plant species and transferred to crops by genetic engineering. Amongst these genes are those that encode inhibitors of proteases (serine and cysteine) and a-amylase, lectins, and enzymes such as chitinases and lipoxygenases.Examples of genetically engineered crops expressing insecticidal plant proteins from di †erent plant species, with enhanced resistance to one or more insect pests from the orders Lepidoptera, Homoptera and Coleoptera are presented. The possibility of "pyramidingÏ di †erent resistance genes to improve the e †ectiveness of protection and durability is discussed and exempliÐed. The number of di †erent crop species expressing such genes is very diverse and ever-increasing. The viability of this approach to crop protection is considered.

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Potentiation of Bacillus thuringiensis insecticidal activity by serine protease inhibitors

Cited by 131 publications

References 22 publications

Effects of Dietary Soybean Trypsin Inhibitors on Detection of Resistance to Pyrethroid and Spinosad Insecticides in Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Effects of Dietary Soybean Trypsin Inhibitors on Detection of Resistance to Pyrethroid and Spinosad Insecticides in Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

Prospects for using transgenic resistance to insects in crop improvement

Identifying proteins with insecticidal activity: use of encoding genes to produce insect-resistant transgenic crops

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