Potentiation of Bleomycin Lethality in HeLa and V79 Cells by Bee Venom

Oršolić, Nada

doi:10.2478/10004-1254-60-2009-1936

Cited by 30 publications

(29 citation statements)

References 35 publications

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“…The same result was observed in Chinese hamster lung fibroblasts subjected to cotreatment with BV and BLM [25]. Moreover, the inhibitory impact of BV solely on cell viability and proliferation was observed in several types of nontumor isolated cells (i.e., normal human lymphocytes) and cell lines (human embryonic kidney HEK-293 cells and normal Hef fibroblasts), as well as tumor cell lines (human laryngeal HEp-2 and cervical carcinoma HeLa cells and their drug resistant sublines, breast adenocarcinoma MCF-7 cells, colon adenocarcinoma SW620 cells, and glioblastoma A1235 cells) [38, 39].…”

Section: Discussionsupporting

confidence: 79%

“…However, BLM increases the risk for a life threating lung injury and a wide variety of chromosomal aberrations in noncancerous cells [21]. In addition to its diverse curative applications, BV has been proven to be a useful compound in potentiating the apoptotic killing capacity of some chemotherapeutic agents such as BLM in cancer cell lines [25, 36]. However, Park et al [37] recorded the antiapoptotic effect of BV against tumor necrosis factor (TNF)- α with actinomycin D-induced apoptosis in normal hepatocyte cell lines.…”

Section: Discussionmentioning

confidence: 99%

“…Previously studied cancer cell lines have illuminated the mitigating effect of BV on the adverse effects of BLM [25]. However, little is known regarding the combined effects of BV and BLM on healthy isolated lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

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Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: AnIn VitroStudy

Abd‐Elhakim

Khalil

Awad

et al. 2014

BioMed Research International

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This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques. Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μg/mL respectively for 24 and 72 hrs. An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects. To determine the predominant type of BV and/or BLM-induced cell death, LDH release assay was employed beside quantitative expression analyses of the apoptosis-related genes (Caspase-3 and Bcl-2). The genotoxic effects of the tested compounds were evaluated via DNA fragmentation assay. The results of these assays demonstrated that BV potentiates BLM-induced cytotoxicity through increased LDH release and diminished cell viability. Nevertheless, BV significantly inhibited the BLM-induced DNA damage. The results verify that BV significantly attenuates the genotoxic effects of BLM on noncancerous isolated rat lymphocytes but does not diminish BLM cytotoxicity.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

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Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: AnIn VitroStudy

Abd‐Elhakim

Khalil

Awad

et al. 2014

BioMed Research International

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“…Orsolic [29], while investigating cytotoxic effects of bee venom applied alone or in combination with the DNA-damaging drug bleomycin on HeLa and V79 cells, found that bleomycin caused a dose-dependent decrease in cell survival. When used with a non-lethal dose of the BV, its lethal effect was potentiated.…”

Section: Discussionmentioning

confidence: 99%

“…When used with a non-lethal dose of the BV, its lethal effect was potentiated. The author inferred that BV, by preventing repair of damaged DNA, increases bleomycin lethality and inhibited recovery from bleomycin-induced damage [29]. …”

Section: Discussionmentioning

confidence: 99%

Potentiation of a novel palladium (II) complex lethality with bee venom on the human T-cell acute lymphoblastic leukemia cell line (MOLT-4)

Safaeinejad

Nabiuni

Nazari

2013

J Venom Anim Toxins incl Trop Dis

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BackgroundAlthough honeybee venom (BV) has been reported to induce apoptosis in different types of cancerous cells, its synergistic effects with customary anti-cancer drugs remain largely unknown. In the present study, we evaluated the cytotoxic effect of BV alone (as a natural product) and the synergistic cytological effects of this component in combination with [Pd (bpy) (Pi-Pydtc)]NO3 – a novel palladium complex on human T-cell lymphoblastic leukemia cells. To investigate the cytotoxic effect of the BV alone and in combination with palladium complex on MOLT-4 cells MTT assay was performed. In order to determine the apoptotic effects of BV separately and in combination with Pd (II) complex on these cells and its ability to induce apoptosis, morphological examination, flowcytometric analysis and caspase-3 colorimetric assay were done.ResultsWe found that BV induced morphological changes, namely nuclear shrinkage, and inhibited MOLT-4 cell proliferation; both effects were dose- and time-dependent. Flow cytometry by Annexin-V antibody demonstrated that BV induced apoptosis in MOLT-4 cells. Furthermore, BV induced apoptosis independently of caspase-3 in these cells. In addition, we proved a clear synergistic effect of BV on [Pd (bpy) (Pi-Pydtc)]NO3. The apoptotic pathway activated by BV in combination with Pd complex was caspase-3-dependent.ConclusionsThese observations provide an explanation for the anti-proliferative properties of BV, and suggest that this agent may be useful for treating lymphoblastic leukemia alone or in combination with chemotherapy drugs pending further investigations on animal models as preclinical tests.

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Combined antitumor effects of bee venom and cisplatin on human cervical and laryngeal carcinoma cells and their drug resistant sublines

Gajski

Čimbora‐Zovko

Rak

et al. 2013

J of Applied Toxicology

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In the present study, we investigated the possible combined anticancer ability of bee venom (BV) and cisplatin towards two pairs of tumour cell lines: parental cervical carcinoma HeLa cells and their cisplatin-resistant HeLa CK subline, as well as laryngeal carcinoma HEp-2 cells and their cisplatin-resistant CK2 subline. Additionally, we identified several peptides of BV in the BV sample used in the course of the study and determined the exact concentration of MEL. BV applied alone in concentrations of 30 to 60 μgml -1 displayed dose-dependent cytotoxicity against all cell lines tested. Cisplatin-resistant cervical carcinoma cells were more sensitive to BV than their parental cell lines (IC 50 values were 52.50 μgml -1 for HeLa vs. 47.64 μgml -1 for HeLa CK cells), whereas opposite results were obtained for cisplatin-resistant laryngeal carcinoma cells (IC 50 values were 51.98 μgml -1 for HEp-2 vs. > 60.00 μgml -1 for CK2 cells). Treatment with BV alone induced a necrotic type of cell death, as shown by characteristic morphological features, fast staining with ethidium-bromide and a lack of cleavage of apoptotic marker poly (ADP-ribose) polymerase (PARP) on Western blot. Combined treatment of BV and cisplatin induced an additive and/or weak synergistic effect towards tested cell lines, suggesting that BV could enhance the killing effect of selected cells when combined with cisplatin. Therefore, a greater anticancer effect could be triggered if BV was used in the course of chemotherapy. Our results suggest that combined treatment with BV could be useful from the point of minimizing the cisplatin concentration during chemotherapy, consequently reducing and/or postponing the development of cisplatin resistance.

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Potentiation of Bleomycin Lethality in HeLa and V79 Cells by Bee Venom

Cited by 30 publications

References 35 publications

Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: AnIn VitroStudy

Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes: AnIn VitroStudy

Potentiation of a novel palladium (II) complex lethality with bee venom on the human T-cell acute lymphoblastic leukemia cell line (MOLT-4)

Combined antitumor effects of bee venom and cisplatin on human cervical and laryngeal carcinoma cells and their drug resistant sublines

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