Potentiation of Photodynamic Therapy with Hypericin by Mitomycin C in the Radiation-induced Fibrosarcoma–1 Mouse Tumor Model¶

Chen, Bin; Ahmed, Bissan; Landuyt, Willy; Ni, Yicheng; Gáspár, Róbert; Roskams, Tania; Witte, Peter de

doi:10.1562/0031-8655(2003)078<0278:poptwh>2.0.co;2

Cited by 10 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Combining MMC with PDT enables the reduction of both light dose and photosensitizer dose . Geel et al studied the combination of MMC and PDT with three different photosensitizers, meso‐tetrahydroxyphenylchlorin (m‐THPC), bateriochlorin α (BCA) and Photofrin .…”

Section: Combining Phototherapy With Chemotherapymentioning

confidence: 99%

Chemophototherapy: An Emerging Treatment Option for Solid Tumors

et al. 2016

View full text Add to dashboard Cite

Near infrared (NIR) light penetrates human tissues with limited depth, thereby providing a method to safely deliver non‐ionizing radiation to well‐defined target tissue volumes. Light‐based therapies including photodynamic therapy (PDT) and laser‐induced thermal therapy have been validated clinically for curative and palliative treatment of solid tumors. However, these monotherapies can suffer from incomplete tumor killing and have not displaced existing ablative modalities. The combination of phototherapy and chemotherapy (chemophototherapy, CPT), when carefully planned, has been shown to be an effective tumor treatment option preclinically and clinically. Chemotherapy can enhance the efficacy of PDT by targeting surviving cancer cells or by inhibiting regrowth of damaged tumor blood vessels. Alternatively, PDT‐mediated vascular permeabilization has been shown to enhance the deposition of nanoparticulate drugs into tumors for enhanced accumulation and efficacy. Integrated nanoparticles have been reported that combine photosensitizers and drugs into a single agent. More recently, light‐activated nanoparticles have been developed that release their payload in response to light irradiation to achieve improved drug bioavailability with superior efficacy. CPT can potently eradicate tumors with precise spatial control, and further clinical testing is warranted.

show abstract

Section: Combining Phototherapy With Chemotherapymentioning

confidence: 99%

Chemophototherapy: An Emerging Treatment Option for Solid Tumors

et al. 2016

View full text Add to dashboard Cite

show abstract

“…The combination of Hyp PDT and mitomycin C, a bioreductive compound that is especially toxic to hypoxic cells, was studied in the RIF-1 tumor model by Chen et al [208]. Hyp PDT alone caused about 60% direct cell killing and significant decreases in tumor perfusion and oxygenation.…”

Section: Immunological Aspects Of Hyp-based Pdtmentioning

confidence: 99%

“…Under these conditions, injection of mitomycin C (2.5 mg.kg -1 , 20 min prior to irradiation) significantly potentiated the cytotoxic effect of Hyp PDT and caused delayed tumor re-growth compared to PDT or mitomycin C treatment alone. Therefore, the combination with a bioreductive drug such as mitomycin C is suggested to enhance tumor cell killing under hypoxic conditions and might be useful to achieve selective and effective tumor control [208].…”

Section: Immunological Aspects Of Hyp-based Pdtmentioning

confidence: 99%

Cellular Mechanisms and Prospective Applications of Hypericin in Photodynamic Therapy

Kiesslich¹,

Krammer²,

Plaetzer

2006

CMC

112

View full text Add to dashboard Cite

During the last decades, Photodynamic Therapy (PDT) has been established as a powerful alternative approved by health agencies of several countries for treatment of various malignant and some non-malignant diseases. PDT makes use of the light-induced destruction of target cells by formation of cytotoxic products in the presence of a photosensitizing agent and oxygen. The light-dependent tumor destructive properties of Hypericin have drawn attention to its promising application as a photosensitizer in the frame of PDT. Hypericin is a naturally occurring secondary metabolite in plants of the Hypericum genus, with Hypericum perforatum (St. John's wort) as it is a commonly known representative. This review focuses on the cellular mechanisms of Hypericin-based phototoxicity and provides an outlook for future application of Hypericin as a fluorescing and photosensitizing agent for diagnosis and treatment of cancerous diseases, respectively.

show abstract

“…Many investigations have demonstrated that PDT with hypericin significantly inhibits tumor growth and induces apoptosis or necrosis in different cell lines (5–9). Besides, in vivo studies demonstrated the efficacy of hypericin‐mediated PDT (10,11) and clinical trials have confirmed the potential of hypericin‐PDT for the treatment of recurrent mesothelioma (12) and basal and squamous cell carcinoma (13). Hypericin has been evaluated in phase I/II clinical trials, in glioblastoma multiform patients, in patients with cutaneous T‐cell lymphoma, and in psoriasis (14).…”

Section: Introductionmentioning

confidence: 98%

Hypericin‐induced Photocytotoxicity is Connected with G2/M Arrest in HT‐29 and S‐Phase Arrest in U937 Cells

Sačková

Fedoročko

Szilárdiová

et al. 2006

Photochem & Photobiology

View full text Add to dashboard Cite

Susceptibility of the HT-29 human colon adenocarcinoma cell line and human myeloid leukemia cell line U937 to hypericin-mediated photocytotoxicity was investigated and compared in this study. Cellular parameters as viability, cell number, metabolic activity and total protein amount were monitored in screening experiments with subsequent cell-cycle analysis and apoptosis detection to determine the cellular response of the different tumor types to various concentrations of photoactivated hypericin. The results show concentration dependence of the photosensitizer's cytotoxicity on the studied cell lines, with higher sensitivity of U937 cells. Whereas the two extreme hypericin concentrations (1 x 10(-9) M and 1 x 10(-6) M) resulted in similar changes in all tested cellular parameters on the two studied cell lines, 1 x 10(-8) M and 1 x 10(-7) M hypericin treatment resulted in different responses of the cell lines in all monitored parameters except for viability. Although leukemic cells proved sensitive to both 1 x 10(-8) M and 1 x 10(-7) M hypericin, significant changes on HT-29 cells were detected only after the 1 x 10(-7) M hypericin concentration. Cell-cycle arrest was related to simultaneously occurring apoptosis in colon cancer. Remarkable is the difference in cell-cycle profile where G2/M arrest in colon cancer cells versus accumulation of leukemic cells in the S phase appears. This suggests that hypericin treatment affecting the cell-cycle machinery of different cancer cells is not universal in effect.

show abstract

Potentiation of Photodynamic Therapy with Hypericin by Mitomycin C in the Radiation-induced Fibrosarcoma–1 Mouse Tumor Model¶

Cited by 10 publications

References 23 publications

Chemophototherapy: An Emerging Treatment Option for Solid Tumors

Chemophototherapy: An Emerging Treatment Option for Solid Tumors

Cellular Mechanisms and Prospective Applications of Hypericin in Photodynamic Therapy

Hypericin‐induced Photocytotoxicity is Connected with G2/M Arrest in HT‐29 and S‐Phase Arrest in U937 Cells

Contact Info

Product

Resources

About