Potentiation of regulatory volume decrease by P2U purinoceptors in HSG-PA cells

Kim, H. D.; Bowen, Jesse W.; James-Kracke, Marilyn R.; Landon, Linda A.; Camden, Jean M.; Burnett, Jane E.; Turner, John T.

doi:10.1152/ajpcell.1996.270.1.c86

Cited by 35 publications

(10 citation statements)

References 12 publications

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“…The influence of ATP as modulator of RVD was first described in hepatoma cells, and salivary gland duct cells (Kim et al, 1996;Wang et al, 1996), and since then, a number of other studies have extended this observation while attempting to elucidate the molecular mechanisms involved in this phenomenon. Controversy still exists as to whether ATP is acting as a necessary signal for the activation of osmolyte efflux pathways (Wang et al, 1996;Light et al, 1999;Musante et al, 1999;Roman et al, 1999a,b;Feranchak et al, 2000;Perez-Samartin et al, 2000;Darby et al, 2003), or if its influence is only exerted once the osmolyte pathways have been already activated ( Van der Wijk et al, 1999;Dezaki et al, 2000;Okada et al, 2001;Rubera et al, 2001;Junankar et al, 2002;Mongin and Kimelberg, 2002;Franco et al, 2004b).…”

Section: Autocrine Regulation Of Cell Volumementioning

confidence: 99%

“…The ATP actions on RVD and osmolyte fluxes appear to involve preferentially the activation of metabotropic purinergic receptors P2Y (Kim et al, 1996;Wang et al, 1996;Roman et al, 1999a,b;Dezaki et al, 2000;Feranchak et al, 2000;Light et al, 2001Light et al, , 2003Shinozuka et al, 2001;Junankar et al, 2002;Darby et al, 2003;Pafundo et al, 2004;Franco et al, 2004b;Mongin and Kimelberg, 2005;Hafting et al, 2006). However, RVD was shown to depend on CFTR mediated ATP release and autocrine signaling through both P2Y and the ionotropic P2X receptors in human airway epithelial cells (Braunstein et al, , 2004.…”

Section: Autocrine Regulation Of Cell Volumementioning

confidence: 99%

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Autocrine signaling involved in cell volume regulation: The role of released transmitters and plasma membrane receptors

Franco

Panayiotidis

Paz

2008

Journal Cellular Physiology

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Cell volume regulation is a basic homeostatic mechanism transcendental for the normal physiology and function of cells. It is mediated principally by the activation of osmolyte transport pathways that result in net changes in solute concentration that counteract cell volume challenges in its constancy. This process has been described to be regulated by a complex assortment of intracellular signal transduction cascades. Recently, several studies have demonstrated that alterations in cell volume induce the release of a wide variety of transmitters including hormones, ATP and neurotransmitters, which have been proposed to act as extracellular signals that regulate the activation of cell volume regulatory mechanisms. In addition, changes in cell volume have also been reported to activate plasma membrane receptors (including tyrosine kinase receptors, G-protein coupled receptors and integrins) that have been demonstrated to participate in the regulatory process of cell volume. In this review, we summarize recent studies about the role of changes in cell volume in the regulation of transmitter release as well as in the activation of plasma membrane receptors and their further implications in the regulation of the signaling machinery that regulates the activation of osmolyte flux pathways. We propose that the autocrine regulation of Ca2+-dependent and tyrosine phosphorylation-dependent signaling pathways by the activation of plasma membrane receptors and swelling-induced transmitter release is necessary for the activation/regulation of osmolyte efflux pathways and cell volume recovery. Furthermore, we emphasize the importance of studying these extrinsic signals because of their significance in the understanding of the physiology of cell volume regulation and its role in cell biology in vivo, where the constraint of the extracellular space might enhance the autocrine or even paracrine signaling induced by these released transmitters.

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Section: Autocrine Regulation Of Cell Volumementioning

confidence: 99%

Section: Autocrine Regulation Of Cell Volumementioning

confidence: 99%

Autocrine signaling involved in cell volume regulation: The role of released transmitters and plasma membrane receptors

Franco

Panayiotidis

Paz

2008

Journal Cellular Physiology

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“…The effect of ATP on various types of preparations from submandibular salivary glands has been reported, including the human submandibular duct cell line, HSG-PA (Yu and Turner 1991;Kim et al 1996;Kurihara et al 1997), rat submandibular gland acini (Hurley et al 1993(Hurley et al , 1994(Hurley et al , 1996 crude cell suspensions of whole rat submandibular glands (Dehaye 1993;Lachish et al 1996;Métioui et al 1996;Turner et al 1997Turner et al , 1998Zeng et al 1997) or ductal cells (Amsallem et al 1996) and a mouse submandibular epithelial salivary cell line, ST 885 (Gibb et al 1994). Studies of rat submandibular gland acini identified, as for parotid acini, a P2X 7 /P2XZ receptor, which promoted Ca 2+ and Na + influx, but not release, from intracellular stores (Hurley et al 1993(Hurley et al , 1996.…”

Section: Exocrine Glandsmentioning

confidence: 99%

Peripheral Nervous System

Burnstock¹,

Verkhratsky

2012

Purinergic Signalling and the Nervous System

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“…Similarly, RVD was activated by UTP in salivary gland duct cells (17) and by ATP, UTP, UDP, or ATP␥S in trout and goldfish hepatocytes (18,19).…”

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confidence: 98%

A Volume Regulatory Response Can Be Triggered by Nucleosides in Human Erythrocytes, a Perfect Osmometer No Longer

Pafundo

Álvarez

Krumschnabel

et al. 2010

Journal of Biological Chemistry

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Human erythrocytes have been regarded as perfect osmometers, which swell or shrink as dictated by their osmotic environment. In contrast, in most other cells, swelling elicits a regulatory volume decrease (RVD) modulated by the activation of purinic and pyrimidinic receptors (P receptors). For human erythrocytes this modulation has not been tested, and we thus investigated whether P receptor activation can induce RVD in these cells. Further, because ectonucleotidases may scavenge ATP or ADP or act as a source for extracellular adenosine and therefore modulate P receptor activation and RVD, we also determined their activity in intact erythrocytes. We found relatively low ectoATPase but significant ectoADPase and ectoAMPase activities. When erythrocytes were exposed to hypotonic medium alone, they swelled as expected for an osmometric response and showed no RVD. Activation of P2 receptors by exogenous ATP or ADP did not trigger RVD, whereas P1 agonists adenosine and adenosine-5-N-ethylcarboxamide induced significant RVD. The effect of adenosine-5-N-ethylcarboxamide was dose-dependent (maximal RVD of 27%; apparent K 1 ⁄ 2 of 1.6 ؎ 1.7 M). The RVD induced by adenosine was blocked 80% with the non-selective P1 antagonist 8-(p-sulfophenyl theophylline) or the P1-A 2B inhibitor MRS1754, but not by inhibitors of P1 subtypes A 1 , A 2A , and A 3 . In addition, forskolin (an inducer of intracellular cAMP formation) could mimic the effect of adenosine, supporting the idea of P1-A 2B receptor activation. In conclusion, we report a novel P1-A 2B receptor-mediated RVD activation in mature human erythrocytes and thus indicate that these long held perfect osmometers are not so perfect after all.

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Potentiation of regulatory volume decrease by P2U purinoceptors in HSG-PA cells

Cited by 35 publications

References 12 publications

Autocrine signaling involved in cell volume regulation: The role of released transmitters and plasma membrane receptors

Autocrine signaling involved in cell volume regulation: The role of released transmitters and plasma membrane receptors

Peripheral Nervous System

A Volume Regulatory Response Can Be Triggered by Nucleosides in Human Erythrocytes, a Perfect Osmometer No Longer

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