Potentiation of the Effect of Paclitaxel and Carboplatin by Antioxidant Mixture on Human Lung Cancer H520 Cells

Abstract

The data suggests that the apoptotic effects of paclitaxel and carboplatin are enhanced by pretreatment with the antioxidant mixture. Thus, the most promising sequence of these agents, which emerged in this study, was pretreatment with antioxidant mixture for 24 hours followed by paclitaxel treatment for 24 hours followed by carboplatin exposure for 24 hours.

Cytotoxic, apoptotic and anti-α-glucosidase activities of 3,4-di-O-caffeoyl quinic acid, an antioxidant isolated from the polyphenolic-rich extract of Elephantopus mollis Kunth.

“…The antioxidative phenolic compounds such as complanatuside A and luteolin were also found to activate apoptosis through death receptor-dependent and mitochondria-dependent pathways in human hepatocarcinoma SMMC-7721 cells (Hu et al, 2009) and human colon carcinoma COLO 201 cells (Imai et al, 2009), respectively. Furthermore, the role of other natural antioxidants such as vitamin C, vitamin E, vitamin A and carotenoids in triggering and regulating of apoptosis were also extensively demonstrated in various cancer cells (Cole and Prasad, 1997;Malafa and Neitzel, 2000;Pathak et al, 2002;Henry et al, 2006).…”

Oxidative stress and apoptosis: Impact on cancer therapy

“…Considerable existing data show increased effectiveness of many chemotherapeutic agents, as well as a decrease in toxic adverse effects, when given concurrently with antioxidants. 21,97,[102][103][104][105][106][107][108] Oxidative stress interferes with cellular processes that are necessary for antineoplastic agents to exert their optimal cytotoxicity on cancer cells, and modest levels of oxidative stress have been shown to reduce the cytotoxicity of anticancer drugs. 5,94 Thus, it was claimed that the formation of ROS that occurs when anticancer drugs are administered may diminish the effectiveness of the treatment.…”

Benzamide riboside induced mitochondrial mediated apoptosis in human lung cancer H520 cells

“…Fixed cells were washed and resuspended in a buffer containing 50 ug/ml propidium iodide [PI], 0.1% sodium citrate, and 1% Triton-X-100. PI stained cells were analyzed using a FACScan cytometer [Coulter] equipped with an argon laser using Win MdI 2.8 software (Pathak et al, 2002).…”