Potentiometric biosensors using immobilized enzyme layers mixed with hydrophilic polyurethane

Shin, Jae Ho; Yoon, Sang Yong; Yoon, In Jun; Choi, Sung Hyuk; Lee, Sung Dong; Nam, Hakhyun; Sig, Geun

doi:10.1016/s0925-4005(98)00151-8

Cited by 44 publications

(26 citation statements)

References 13 publications

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“…3). This detection limit is markedly lower than those (50-100µM) previously reported for pH-FET biosensors [1,2] and similar to the value (1µM) recently obtained with potentiometric biosensors [23]. It appears clearly that the better detection limit is obtained when the detected specie is NH 4 + .…”

Section: Analytical Characteristics Of the Urea Sensor Based On Ammonsupporting

confidence: 84%

Development of new polymeric membranes for ENFETs for biomedical and environmental applications

Jaffrézic‐Renault¹,

Wan²,

Senillou³

et al. 1999

Analusis

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Two ways have been investigated for the preparation of urea ENFETs : covalent bonding of urease directly on the surface of an ammonium sensitive FET and control of the diffusion of charged species into the enzymatic layer on pH-ISFET using additional charged polymeric membranes. Trichlorfon as a representative pesticide is detected using a BuChE-FET sensor which is prepared either with cross-linked BSA-glutaraldehyde membrane or photocross-linkable PVA/SbQ (poly(vinyl alcohol) containing styrylpyridinium) membrane.

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Section: Analytical Characteristics Of the Urea Sensor Based On Ammonsupporting

confidence: 84%

Development of new polymeric membranes for ENFETs for biomedical and environmental applications

Jaffrézic‐Renault¹,

Wan²,

Senillou³

et al. 1999

Analusis

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“…Typically photo-linking prepared polymers are used (Jae Ho Shin et al, 1998;Jobst et al, 1993;Nakako at al., 1986;Barie et al, 1998;Dobrikov & Shishkin, 1983a, 1983bDontha et al, 1997;Leca et al, 1995;Nakayama & Matsuda, 1992;Nakayama et al, 1995;Navera et al, 1991). Polyvinyl derivatives such as polyvinyl chloride were widely used at the creation of biosensors (Jae Ho Shin et al, 1998).…”

Section: Photo Linking In Biosensoricsmentioning

confidence: 99%

“…Polyvinyl derivatives such as polyvinyl chloride were widely used at the creation of biosensors (Jae Ho Shin et al, 1998). It was communicated about photo-linked polyvinyl alcohol in aqueous solution for the immobilization of cells of Arthrobacter globiformis.…”

Section: Photo Linking In Biosensoricsmentioning

confidence: 99%

Photopolymerizable Materials in Biosensorics

Starodub¹

2011

Environmental Monitoring

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“…A variety of ammonium ion-selective electrodes has been developed [19]. To overcome most of the obstacles faced by potentiometric assays a number of laboratory prototypes of amperometric ammonium sensors have been developed with ion-selective components [23] and enzymes [3].…”

Section: Introductionmentioning

confidence: 99%

Direct detection of ammonium ion by means of oxygen electrocatalysis at a copper-polyaniline composite on a screen-printed electrode

Zhybak

Vagin

Beni³

et al. 2016

Microchim Acta

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We describe a composite material for use in electrochemical oxygen reduction. A screenprinted electrode (SPE) was consecutively modified with electrodeposited copper, a Nafion membrane and electropolymerised polyaniline (PANi) to give an electrocatalytic composite of type PANi/Nafion/Cu2O/SPE that displays good electrical conductivity at neutral pH values. It is found that the presence of ammonia causes complex formation with Cu(I), and this causes electroreduction of oxygen to result in an increased cathodic current. The finding was applied to the quantification of ammonium ions in the 1 to 1000 µM concentration range by amperometry at -0.45 V (vs. Ag/AgCl). This Faradaic phenomenon offers the advantage of direct voltammetric detection, one of the lowest known limits of detection (0.5 µM), and high sensitivity (250 mA•M -1 •cm -2 ). It was applied to the determination of ammonium ion in human serum where it compared well with the photometric routine approach for clinical analysis using glutamate dehydrogenase.

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Potentiometric biosensors using immobilized enzyme layers mixed with hydrophilic polyurethane

Cited by 44 publications

References 13 publications

Development of new polymeric membranes for ENFETs for biomedical and environmental applications

Development of new polymeric membranes for ENFETs for biomedical and environmental applications

Photopolymerizable Materials in Biosensorics

Direct detection of ammonium ion by means of oxygen electrocatalysis at a copper-polyaniline composite on a screen-printed electrode

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