Potentiometric competitive immunoassay for determination of aflatoxin B1 in food by using antibody-labeled gold nanoparticles

Li, Qunfang; Lu, Minghua; Lin, Zhenzhen; Tang, Dianping

doi:10.1007/s00604-016-1929-x

Cited by 41 publications

(9 citation statements)

References 27 publications

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“…Table 1 summarizes the comparison of a few methods for detection of AFB1 using antibodies or aptamers. The sensitivity of our aptamer-based SPR assay is better than that reported antibody SPR assay [30,34], and comparable to that of competitive potentiometric immunoassay [7]. Compared with previous work using the same kind of aptamer with longer sequence (showing similar binding affinity), our detection limit is close to that reported by colorimetric assay based on DNAzyme [16], competitive chemiluminescence assay [17].…”

Section: Spr Responses To Afb1supporting

confidence: 66%

“…Detection of AFB1 is important in food safety, quality control, environmental analysis, and toxin sensing. Currently, the existing methods for AFB1 detection are mainly based on high performance liquid chromatography (HPLC) [3] and immunoassay [4][5][6][7]. HPLC coupled with fluorescence detection or mass spectrometry has strength in high accuracy and good repeatability, but it usually needs time-consuming and laborious process.…”

Section: Introductionmentioning

confidence: 99%

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Aptamer based surface plasmon resonance sensor for aflatoxin B1

Sun

Zhao

2017

Microchim Acta

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The authors describe a surface plasmon resonance (SPR) based aptasensor for the carcinogenic mycotoxin aflatoxin B1 (AFB1) in a direct assay format. The aptamer is immobilized on the surface of a commercial sensor chip, and the SPR signal increases on binding of AFB1. The sensor chip can be fully regenerated by passing a flow of buffer over it upon which bound AFB1 dissociates from the aptamer. The biosensor works in the 0.4 nM to 200 nM AFB1 concentration range and has a 0.4 nM detection limit. It allows AFB1 to be determined in complex samples such as diluted red wine and beer. The assay is sensitive, and the chip is easily regenerated and stable. The method therefore overcomes certain limitations of antibody-based SPR assays and of competitive SPR assays for AFB1.

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Section: Spr Responses To Afb1supporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Aptamer based surface plasmon resonance sensor for aflatoxin B1

Sun

Zhao

2017

Microchim Acta

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“…Compared with other typical methods, the results in this work demonstrate an excellent sensitivity for the detection of AFB1. Although the LOD was somewhat higher than those of some methods (Potentiometric immunosensor, Li, Lv, Lu, Lin, & Tang, 2016), it offered relative advantages of low cost, simpler operation procedure and good reproducibility, meanwhile, the detection time is short. The results are listed in Table 1.…”

Section: Evaluation Of the Methodologymentioning

confidence: 89%

Development and evaluation of the magnetic particle-based chemiluminescence immunoassay for rapid and quantitative detection of Aflatoxin B1 in foodstuff

Xie

Zhu

Liu

et al. 2017

Food and Agricultural Immunology

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Herein, a simple and cost-effective chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) is presented for the rapid detection and analysis of Aflatoxin B1, which is a potent carcinogen found in the environment. Several physicochemical factors that potentially influence the assay performance of the proposed method were investigated in detail, including concentrations of FITC-labelled antibody, AFB1-alkaline phosphatase and magnetic particles concentration. Under the optimized experiment condition, the method showed: (i) a low limit detection of 0.05 ng/mL; (ii) satisfactory recoveries in real grain and oil samples ranging from 78% to 109%, which was well within the requirement of residue analysis, and (iii) excellent sensitivity, wide dynamic range and easy to separate in the washing process, which was superior to enzyme-linked immunosorbent assay (ELISA) method. In addition, the device used is of low cost. Moreover, the MPCLIA results for the real samples were in good agreement with that obtained by the high-performance liquid chromatography. These results suggest that the proposed MPCLIA method has potential application for screening other toxic mycotoxins occur in the food industry or other applied fields.

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“…17,18 Hence, this study may provide a possibility of studying other reactions by controlling the release of suitable reagents such as antigens or toxins. 28,29 The inset of Fig. 3 shows the obtained k values in correspondence to different pH conditions, which are usually ascribed to the distribution of the acid-base species of the reactants.…”

Section: Resultsmentioning

confidence: 99%