Potentiometric nanosensor for real-time measurement of hydrogen sulfide in single cell

Cheng, Shuwen; Zhang, Shuai; Liu, Rantong; Zeng, Hui; Yin, Yongyue; Zhang, Meining

doi:10.1039/d2cc06557f

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…It can be seen that the potential has little changes with the addition of BSA. This high antifouling property benefit from the sensitivity of potentiometry is independent of the electrochemical surface area. ,− This advantage is beneficial in monitoring the dynamics of neurochemicals for long-term implantation.…”

Section: Results and Discussionmentioning

confidence: 99%

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca²⁺ in Rat Brain

et al. 2023

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In vivo electrochemical analysis is of great significance in understanding the dynamics of various physiological and pathological activities. However, the conventional microelectrodes for electrochemical analysis are rigid and permanent, which comes with increased risks for long-term implantation and secondary surgery. Here, we develop one biodegradable microelectrode for monitoring the dynamics of extracellular Ca 2+ in rat brain. The biodegradable microelectrode is prepared by sputtering gold nanoparticles (AuNPs) on a wet-spun flexible poly(L-lactic acid) (PLLA) fiber for conduction and transduction and coating a Ca 2+ ion-selective membrane (ISM) with a PLLA matrix on the PLLA/AuNPs fiber, forming PLLA/AuNPs/Ca 2+ ISME (ISME = ion-selective microelectrode). The prepared microelectrode shows excellent analytical properties including a near-Nernst linear response toward Ca 2+ over the concentration range from 10 μM to 50 mM, good selectivity, and long-term stability for weeks as well as biocompatibility and biodegradability. The PLLA/AuNPs/ Ca 2+ ISME can monitor the dynamics of extracellular Ca 2+ following spreading depression induced by high potassium even if in the fourth day. This study provides a new design strategy for the biodegradable ISME and promotes the development of biodegradable microelectrodes for long-term monitoring of chemical signals in brain.

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Section: Results and Discussionmentioning

confidence: 99%

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca²⁺ in Rat Brain

et al. 2023

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Reference‐Attached pH Nanosensor for Accurately Monitoring the Rapid Kinetics of Intracellular H⁺ Oscillations

Wen,

Qi,

Jiao

et al. 2024

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Intracellular pH (pHi) is an essential indicator of cellular metabolic activity, as its transient or small shift can significantly impact cellular homeostasis and reflect the cellular events. Real‐time and precise tracking of these rapid pH changes within a single living cell is therefore important. However, achieving high dynamic response performance (subsecond) pH detection inside a living cell with high accuracy remains a challenge. Here a reference‐attached pH nanosensor (R‐pH‐nanosensor) with fast and precise pHi sensing performance is introduced. The nanosensor comprises a highly conductive H+‐sensitive IrRuOx nanowire (SiC@IrRuOx NW) as the intracellular working electrode and a SiC@Ag/AgCl NW as an intracellular reference electrode (RE) to diminish the interferences arising from cell membrane potential fluctuations. This whole‐inside‐cell detection mode ensures that the entire potential detection circuit is located within the same cell, and the R‐pH‐nanosensor is able to quantify the mild acidification of cytosol and completely record the fast pH variation within a single cell. It also enables real‐time potentiometric monitoring of the pHi oscillations, which synchronize with the glycolysis oscillations in cancer cells. Furthermore, the asymmetry in glycolysis oscillations wave is disclosed and the inhibitory effect of just lactate to glycolysis oscillations is further confirmed.

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Development of a Photoelectrochemical Microelectrode Using an Organic Probe for Monitoring Hydrogen Sulfide in Living Brains

Hao,

Yang,

Wang

et al. 2024

Anal. Chem.

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Hydrogen sulfide (H 2 S) is an important bioactive molecule that plays a significant role in various functions, particularly in the living brain, where it is closely linked to cognition, memory, and several neurological diseases. Consequently, developing effective detection methods for H 2 S is essential for studying brain functions and the underlying mechanisms of these diseases. This study aims to construct a novel photoelectrochemical (PEC) microelectrode Ti/TiO 2 @HSP for the quantitative monitoring of H 2 S levels in the living brain. The PEC microelectrode Ti/TiO 2 @HSP is formed by covalently bonding a specifically designed organic PEC probe HSP, which possesses a Dπ−A structure, to the surface of TiO 2 nanotubes generated via in situ anodic oxidation of titanium wire. The PEC probe HSP can effectively react with H 2 S and generate significant photocurrent response under long-wavelength excitation light (560 nm), thereby achieving quantitative detection of H 2 S. The sensor demonstrates high sensitivity and good selectivity. In vivo experiments utilizing the PEC microelectrode Ti/TiO 2 @HSP enable the monitoring of dynamic changes in H 2 S levels across various regions of the mouse brain. The findings reveal that in normal mice, the concentration of H 2 S in the hippocampus is significantly higher than in the striatum and cerebral cortex. Additionally, following propargylglycine drug stimulation, H 2 S concentrations in different brain regions were observed to decrease, with the most substantial reduction noted in the hippocampus. This suggests that cystathionine γ-lyase (CSE) is the primary enzyme responsible for H 2 S production in this area, while the striatum exhibits a less pronounced decrease in H 2 S concentration, indicating a reliance on alternative enzymatic pathways for H 2 S production. Therefore, this study not only successfully develops a high-performance H 2 S detection sensor but also provides new experimental tools and theoretical foundations for further exploring the roles of H 2 S in neurophysiological and pathological processes.

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Potentiometric nanosensor for real-time measurement of hydrogen sulfide in single cell

Abstract: One potentiometric nanosensor for monitoring intracellular hydrogen sulfide (H2S) with fast potential response, high selectivity and excellent antifouling property was developed. This study constructs a powerful tool to real-time track...

Cited by 4 publications

References 32 publications

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca²⁺ in Rat Brain

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca²⁺ in Rat Brain

Reference‐Attached pH Nanosensor for Accurately Monitoring the Rapid Kinetics of Intracellular H⁺ Oscillations

Development of a Photoelectrochemical Microelectrode Using an Organic Probe for Monitoring Hydrogen Sulfide in Living Brains

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Potentiometric nanosensor for real-time measurement of hydrogen sulfide in single cell

Abstract: One potentiometric nanosensor for monitoring intracellular hydrogen sulfide (H2S) with fast potential response, high selectivity and excellent antifouling property was developed. This study constructs a powerful tool to real-time track...

Cited by 4 publications

References 32 publications

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca2+ in Rat Brain

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca2+ in Rat Brain

Reference‐Attached pH Nanosensor for Accurately Monitoring the Rapid Kinetics of Intracellular H+ Oscillations

Development of a Photoelectrochemical Microelectrode Using an Organic Probe for Monitoring Hydrogen Sulfide in Living Brains

Contact Info

Product

Resources

About

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca²⁺ in Rat Brain

Biodegradable Microelectrodes for Monitoring the Dynamics of Extracellular Ca²⁺ in Rat Brain

Reference‐Attached pH Nanosensor for Accurately Monitoring the Rapid Kinetics of Intracellular H⁺ Oscillations