Potentiometric stripping analysis

Jagner, Daniel; Granéli, Anders

doi:10.1016/s0003-2670(01)84627-4

Cited by 205 publications

(85 citation statements)

References 2 publications

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“…Blood lead analysis was carried out using anodic stripping voltammetry on an ESA-3010B lead analyser (17). Haemoglobin was estimated using the Drabkins method (18), ALAD activity was measured using the European standardized method (19), ZPP was estimated using front-face fluorometry on a haematofluorometer (20) and serum aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transpeptidase (γ-GT), bilirubin, total proteins, urea, uric acid, creatinine, calcium and phosphorous were estimated using fully automated methods on an Excel-300 ERBA auto analyzer.…”

Section: Methodsmentioning

confidence: 99%

Lead Toxicity in a Family as a Result of Occupational Exposure

Raviraja¹,

Babu²,

Bijoor³

et al. 2008

Archives of Industrial Hygiene and Toxicology

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This article describes an entire family manufacturing lead acid batteries who all suffered from lead poisoning. The family of five lived in a house, part of which had been used for various stages of battery production for 14 years. Open space was used for drying batteries. They all drank water from a well located on the premises. Evaluation of biomarkers of lead exposure and/or effect revealed alarming blood lead levels [(3.92±0.94) , while blood pressure was high in the head of the family and his wife and normal in children. Lead concentration in well water was estimated to 180 µg L -1 . The family was referred to the National Referral Centre for Lead Poisoning in India, were they were received treatment and were informed about the hazards of lead poisoning. A follow up three months later showed a slight decrease in blood lead levels and a significant increase in haemoglobin. These findings can be attributed to behavioural changes adopted by the family, even though they continued producing lead batteries.

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Section: Methodsmentioning

confidence: 99%

Lead Toxicity in a Family as a Result of Occupational Exposure

Raviraja¹,

Babu²,

Bijoor³

et al. 2008

Archives of Industrial Hygiene and Toxicology

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“…8 It has been applied to metal analysis and bioanalysis. 9,10 In this work, electrochemical methods, i.e., potentiometric stripping analysis (PSA) and cyclic voltammetry (CV), were used to investigate the electrochemical behavior of breast cancer cells and to evaluate the influence of diosgenin on the viability and proliferation of breast cancer cells.…”

mentioning

confidence: 99%

Electrochemical Study of Breast Cancer Cells MCF-7 and Its Application in Evaluating the Effect of Diosgenin

Liu

Guo

et al. 2005

ANAL. SCI.

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Cell growth and the effect of some drugs on cells can be evaluated by traditional morphological observation methods or by biological technology.However, to perform these experiments, complex instruments and professionals are always required.Recent studies indicated that electrochemical methods, such as CV, scanning electrochemical microscopy, electric impedance and oxygen electrode, could also provide useful information on the living cell because the cell viability has positive relation with its electrode behavior. 1-4As a plant steroid produced by secondary metabolism, diosgenin shows many important biological functions in plants and animals, acting as a regulator of developmental and physiological processes in animal system. 5It is also widely used to synthesize new steroidal drugs. 6 Recently, some studies have concerned its utilization in cancer therapy. 7 However, to our knowledge, few reports about the effect of diosgenin on the breast cancer cells can be found in the literature.Potential stripping analysis, first introduced by Jagner and Granile in 1976, is a highly sensitive electroanalysis technology including two steps: (1) preconcentration of the analyte by electrodeposition; and (2) stripping of the analyte by an appropriate oxidant. 8 It has been applied to metal analysis and bioanalysis. 9,10 In this work, electrochemical methods, i.e., potentiometric stripping analysis (PSA) and cyclic voltammetry (CV), were used to investigate the electrochemical behavior of breast cancer cells and to evaluate the influence of diosgenin on the viability and proliferation of breast cancer cells. Trypan Blue dyeing tests were also employed to verify the results obtained by PSA. Experimental ChemicalsDiosgenin was from the Institute of Life Science and Technology of Hunan University. A stock solution of 1.0 × 10 -2 M diosgenin was prepared with ethanol and diluted to desired concentrations with water. Phosphate buffer solution (PBS) composed of 136.7 mM NaCl, 2.7 mM KCl, 9.7 mM Na2HPO4 and 1.5 mM KH2PO4 was used as the background electrolyte in all experiments. Other chemicals are of analytical grade. InstrumentationAll electrochemical measurements were performed at a CHI 660A electrochemical analyzer (Chenhua Company, China) by applying a three-electrode system, with a graphite electrode (0.45 cm 2 ) as working electrode, platinum as counter electrode and Ag/AgCl electrode as reference electrode. Cell culture and cell treatmentBreast cancer cell line MCF-7 was kindly provided by Dr. K. Q. Deng (Institute of Life Science and Technology of Hunan University) and was routinely cultured in RPMI-1640 medium (Invitrogen Corporation) + 10% fetal bovine serum (Sigma) + 0.05 mg mL -1 gentamicin (Sigma) + 100 U mL -1 penicillin (Sigma) at 37˚C in a CO2 incubator containing 5% CO2.After the breast cancer cells were cultured for five days, they were collected by trypsinization with 0.05% (v/v) trypsin (Sigma) and centrifugation at 1200 rpm for 10 min, and then suspended in PBS. It should be mentioned that PBS-containing cells s...

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“…The extracts were analised for of Cu, Pb and Cd by Potentiometric Stripping and Zn by Atomic Absorption Spectrophotometry (Talbot, 1983;Jagner & Aren, 1979;Jagner, 1982;Cavalcante et al, 1994). The results of the analytical determinations of the replicates were expressed in dry weight.…”

Section: Methodsmentioning

confidence: 99%