Potentiometric Study of Antioxidant Activity: Development and Prospects

Abstract

The increasing interest in the study of the antioxidant activity of different objects is caused by an unbalance between the formation of reactive oxygen species (ROS) and the performance of the antioxidant system in humans under certain conditions, which leads to oxidative stress and pathological states of the organism. This article presents a brief critical review of the methods that are used to measure integrated antioxidant activity (AOA). It is shown that the most promising methods for measuring AOA are electrochemical ones, particularly potentiometry, as it best fits the nature of the processes causing oxidative stress. The article gives the theoretical rational for requirements that an oxidizer of antioxidants (AO) should meet. The work presents the thermodynamic grounds for the use of an earlier proposed mediator system, kinetics of chemical reactions between AO and the mediator system. In order to confirm reliability and accuracy of the results, numerous correlation studies were conducted, aiming to compare the data obtained with the use the proposed method and independent analytical methods. The article presents the results of the potentiometric study of AOA for a variety of objects, including individual antioxidant → nutritional supplements → food → blood and blood fractions.

Optimized Potentiometric Assay for Non‐invasive Investigation of Skin Antioxidant Activity

“…Solutions of the mediator system and its oxidized form was investigated as variants for replacing the gel. Since the rate of interaction of skin antioxidants with potassium hexacyanoferrate (III) in the aqueous medium is higher than in the gel , the use of reagent solution would allow increasing the detected signal and shortening the measurement time.…”

Antioxidant Capacity of Anthocyanin Pigments

“…It is interesting to mention the fact that electrochemical [243][244][245][246][247][248][249] and ESR [250] methods are increasingly being applied to the determination of antioxidant capacity. The kind of technology and free radical generator or oxidant influences the antioxidant capacity measurement.…”

Ferulic Acid and Naturally Occurring Compounds Bearing a Feruloyl Moiety: A Review on Their Structures, Occurrence, and Potential Health Benefits

“…Several authors make a distinction between “antioxidant capacity” and “antioxidant activity,” stating that the former measures the moles of given free radical scavengers by a test solution, while the 2nd refers to a rate constant of the antioxidant effect on free radicals. In addition, other terms such as “antioxidant power” and “antioxidant ability” are also used, and they take into account the antioxidant effect (Ivanova and others ).…”