Pouteria ramiflora leaf extract on emulgel in wound healing activity in diabetic rats

Abstract

The genus Pouteria has been studied because it presents various activities, among which is its anti-inflammatory potential. The effects of Pouteria ramiflora Carbopol gel on the healing of skin wounds in diabetic rats were evaluated by microscopic imaging. Streptozotocin was administered intraperitoneally in animals that had fasted for 12 hours, a situation confirmed by the glycemic index (˃ 240 mg dL-1). An excision on the back of the animals was performed and three groups were formed: Control (Gel), Ethanolic extract (Ext) and Gel + extract 2% (Ext+gel); the histopathological evaluation occurred on the 7th, 14th, 21st and 30th days after the post-operative period. The results of the phytochemical prospecting of P. ramiflora extract demonstrated the major presence of phenolic compounds and flavonoids; the assessment of the inflammatory infiltrate on the 7th day was higher on group Ext and Ext+gel when compared to group Control; on the 14th day control and Ext (p<0.05). The quantification of fibroblasts was higher on the 7th day among the three treatments, control and Ext (p<0.05), on the 21st day. Angiogenesis showed a higher number of vessels in Ext+gel group (p<0.05) on the 7th day; in Control, Ext and Ext+gel (p<0.05) on the 14th day; and Control and Ext (p<0.05)on the 21st day. The histopathological results showed that the formulation Ext+gel was efficient in tissue reparation and decrease in inflammatory cells on the diabetic’s animals.

Evaluation of Wound Healing Activity of <i>Vernonia amygdalina</i> Extract in Diabetic Rats

“…Chemotaxis and phagocytosis lead to a reduced ability of wounds to heal and a higher risk of infection in people with Diabetes Mellitus (DM), there is not only an increase in glycemic index level, but also a decrease in permeability and vascular flow, and this affects both the synthesis of collagen fibres and the remodelling of wounded tissue. Increased blood sugar levels have been linked to endothelial damage 14 . Wound healing processes require coordination of overlapping distinct cellular activities, involving phagocytosis, chemotaxis, mitogenesis, angiogenesis, synthesis of collagen and other matrix constituents, and interaction of extracellular matrix molecules, soluble mediators, and various cells that cooperate in the repair procedure 15 .…”

Scientific and technological prospection of Pilocarpus microphyllus and epiisopilothurin related to their anti-inflammatory activity in wound treatment

“…PUBMEDAo utilizar o descritor "feridas", foram resgatados 790 artigos, os quais foram publicados no período entre 1983 e 2021 distribuídos com o maior número em 2010 (6,3%), 2009 (6,2%) e 2006 (5,8%) (figura 4). Os artigos abordam sobre a busca de evidências relacionadas à eficácia e segurança do uso de Aloe vera no tratamento de queimaduras (ZAGO, et al, 2021), efeitos do extrato etanólico das folhas de Pouteria ramiflora na cicatrização de feridas cutâneas em ratos diabéticos(Corrêa, et al, 2021), o uso do ozônio como terapia de suporte para auxiliar no reparo tecidual (Santo, et al, 2021) e outros, demonstrando que trata-se de um tema relevante.…”