Powdery Mildew of Prairie Gentian: Characteristics, Molecular Phylogeny and Pathogenicity

Okamoto, Jun; Limkaisang, Saranya; Nojima, Hidenobu; Takamatsu, Susumu

doi:10.1007/pl00013077

Cited by 13 publications

(11 citation statements)

References 18 publications

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“…ITS sequences of Group II were most similar to those of the E. trifolii complex (Takamatsu et al. , 1999; Okamoto et al. , 2002; Cunnington et al.…”

Section: Discussionmentioning

confidence: 84%

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Erysiphe trifolii– a newly recognized powdery mildew pathogen of pea

et al. 2010

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Diversity of powdery mildew pathogens infecting pea (Pisum sativum) in the US Pacific Northwest was investigated using both molecular and morphological techniques. Phylogenetic analyses based on rDNA ITS sequences, in combination with assessment of morphological characters, defined two groups of powdery mildews infecting pea. Group I (five field samples and three glasshouse samples) had ITS sequences 99% similar to those of Erysiphe pisi in GenBank and exhibited simple, mycelioid type of chasmothecial appendages typical of E. pisi. Erysiphe pisi is normally considered as the powdery mildew pathogen of pea. Group II (four glasshouse samples and two field samples) had ITS sequences 99% similar to those of E. trifolii and produced chasmothecia with dichotomously branched appendages similar to those of E. trifolii. There are fourteen nucleotide differences in the ITS region between the two groups. The correlation of rDNA ITS sequences with teleomorphic features for each of the two groups confirms their identity. Repeated samplings and artificial inoculations indicate that both E. pisi and E. trifolii infect pea in the US Pacific Northwest. Erysiphe trifolii is not previously known as a pathogen of pea. The existence of two distinct powdery mildew species infecting pea in both glasshouse and field environments may interfere with the powdery mildew-resistance breeding programmes, and possibly explains putative instances of breakdown of resistance in previously resistant pea breeding lines.

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“…ITS sequences of Group II were most similar to those of the E. trifolii complex (Takamatsu et al. , 1999; Okamoto et al. , 2002; Cunnington et al.…”

Section: Discussionmentioning

confidence: 84%

“…from three different hosts from Japan which were in an E. bauemleri / E. trifolii clade (Fig. 1 in Okamoto et al. , 2002).…”

Section: Resultsmentioning

confidence: 99%

Erysiphe trifolii– a newly recognized powdery mildew pathogen of pea

et al. 2010

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“…Takam., based on rDNA ITS sequences . Okamoto et al (2002) suggested a close relationship of Oidium subgenus Pseudoidium on prairie gentian (Eustoma grandiflorum) with E. baeumleri (Magnus) U. Braun & S. Takam.…”

Section: Introductionmentioning

confidence: 96%

Molecular phylogenetic and morphological analyses of Oidium heveae, a powdery mildew of rubber tree

et al. 2005

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Powdery mildew of rubber tree caused by Oidium heveae is an important disease of rubber plantations worldwide. Identification and classification of this fungus is still uncertain because there is no authoritative report of its morphology and no record of its teleomorphic stage. In this study, we compared five specimens of the rubber powdery mildew fungus collected in Malaysia, Thailand, and Brazil based on morphological and molecular characteristics. Morphological results showed that the fungus on rubber tree belongs to Oidium subgen. Pseudoidium. Nucleotide sequence analysis of the ribosomal DNA internal transcribed spacer (ITS) region and the large subunit rRNA gene (28S rDNA) were conducted to determine the relationships of the rubber powdery mildew fungus and to link this anamorphic fungus with its allied teleomorph. The results showed that the rDNA sequences of the two specimens from Malaysia were identical to a specimen from Thailand, whereas they differed by three bases from the two Brazilian isolates: one nucleotide position in the ITS2 and two positions in the 28S sequences. The ITS sequences of the two Brazilian isolates were identical to sequences of Erysiphe sp. on Quercus phillyraeoides collected in Japan, although the 28S sequences differed at one base from sequences of this fungus. Phylogenetic trees of both rDNA regions constructed by the distance and parsimony methods showed that the rubber powdery mildew fungus grouped with Erysiphe sp. on Q. phillyraeoides with 100% bootstrap support. Comparisons of the anamorph of two isolates of Erysiphe sp. from Q. phillyraeoides with the rubber mildew did not reveal any obvious differences between the two powdery mildew taxa, which suggests that O. heveae may be an anamorph of Erysiphe sp. on Q. phillyraeoides. Cross-inoculation tests are required to substantiate this conclusion.

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“…Recently, molecular analysis based on nucleotide sequences of the nuclear rDNA has been used to determine the phylogenetic relationship among powdery mildew fungi (Takamatsu et al 1998(Takamatsu et al , 1999Saenz and Taylor 1999;Braun and Takamatsu 2000;Mori et al 2000;Kiss et al 2001;Okamoto et al 2002;Matsuda and Takamatsu 2003;Takamatsu 2004). This technique can be applied to investigate the relationship between an anamorphic species and its suspected teleomorphic stage (Cunnington 2002;Cunnington et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogenetic analyses reveal a close relationship between powdery mildew fungi on some tropical trees and Erysiphe alphitoides, an oak powdery mildew

Limkaisang¹,

Takamatsu²,

Cunnington³

et al. 2006

Mycoscience

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To investigate the phylogenetic relationships among the powdery mildew fungi of some economically important tropical trees belonging to Oidium subgenus Pseudoidium, we conducted molecular phylogenetic analyses using 30 DNA sequences of the rDNA internal transcribed spacer (ITS) regions and 26 sequences of the domains D1 and D2 of the 28S rDNA obtained from the powdery mildews on Hevea brasiliensis (para rubber tree), Anacardium occidentale (cashew), Bixa orellana, Citrus spp., Mangifera indica (mango), and Acacia spp. The results indicate that the powdery mildew fungi isolated from these tropical trees are closely related to one another. These powdery mildews are also closely related to E. alphitoides (including Erysiphe sp. on Quercus phillyraeoides). Because of the obligate biotrophic nature of the powdery mildew fungi, the relationship between powdery mildews and their host plants is conservative. However, the present study suggests that a particular powdery mildew species has expanded its host ranges on a wide range of the tropical trees. This article also suggests that a powdery mildew fungus distributed in temperate regions of the Northern Hemisphere expanded its host ranges onto tropical plants and may be a good example of how geographical and host range expansion has occurred in the Erysiphales.

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Powdery Mildew of Prairie Gentian: Characteristics, Molecular Phylogeny and Pathogenicity

Cited by 13 publications

References 18 publications

Erysiphe trifolii– a newly recognized powdery mildew pathogen of pea

Erysiphe trifolii– a newly recognized powdery mildew pathogen of pea

Molecular phylogenetic and morphological analyses of Oidium heveae, a powdery mildew of rubber tree

Molecular phylogenetic analyses reveal a close relationship between powdery mildew fungi on some tropical trees and Erysiphe alphitoides, an oak powdery mildew

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