Powdery mildew susceptibility of spring wheat cultivars as a major constraint on grain yield

Draz, Ibrahim Sobhy; Esmail, Samar M.; Abou-Zeid, Mohamed A.; Essa, Tarek A.

doi:10.1016/j.aoas.2019.05.007

Cited by 35 publications

(19 citation statements)

References 31 publications

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“…In Egypt, this pathogen causes 1.96 to 8.21% losses in yield of most of the local wheat varieties [5]. In Egypt, leaf rust caused by Puccinia triticina could cause yield loss of 5-10% or more in commercial wheat cultivars as they lack adequate resistance to the pathogen [6,7].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity, antifungal evaluation and molecular docking studies of Cu-chitosan nanoparticles as prospective stem rust inhibitor candidates among some Egyptian wheat genotypes

et al. 2021

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Wheat has a remarkable importance among cereals worldwide. Wheat stem and leaf rust constitute the main threats that destructively influence grain quality and yield production. Pursuing resistant cultivars and developing new genotypes including resistance genes is believed to be the most effective tool to overcome these challenges. This study is the first to use molecular markers to evaluate the genetic diversity of eighteen Egyptian wheat genotypes. Moreover, the molecular docking analysis was also used to assess the Cu-chitosan nanoparticle (CuChNp) treatment and its mode of action in disease control management. The tested genotypes were categorized into two main cluster groups depending on the similarity matrix, i.e the most resistant and susceptible genotypes to stem and leaf rust races. The results of SCoT primers revealed 140 polymorphic and 5 monomorphic bands with 97% polymorphism. While 121 polymorphic and 74 monomorphic bands were scored for SRAP primers (99% polymorphism). The genotypes Sakha 94, Sakha 95, Beni Sweif 4, Beni Sweif 7, Sohag 4 and Sohag 5 were resistant, while Giza 160 was highly susceptible to all stem rust races at the seedling stage. However, in the adult stage, the 18 genotypes were evaluated for stem and leaf rust-resistant in two different locations, i.e. Giza and Sids. In this investigation, for the first time, the activity of CuChNp was studied and shown to have the potential to inhibit stem and leaf rust in studied Egyptian wheat genotypes. The Spraying Cu-chitosan nanoparticles showed that the incubation and latent periods were increased in treated plants of the tested genotypes. Molecular modeling revealed their activity against the stem and leaf rust development. The SRAP and SCoT markers were highly useful tools for the classification of the tested wheat genotypes, although they displayed high similarities at the morphological stage. However, Cu-chitosan nanoparticles have a critical and effective role in stem and leaf rust disease control.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity, antifungal evaluation and molecular docking studies of Cu-chitosan nanoparticles as prospective stem rust inhibitor candidates among some Egyptian wheat genotypes

et al. 2021

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“…tritici (Bgt), respectively [5,6]. Both fungi grow usually only on the corresponding host [7] while infection with either Bgt or Bgh can lead to severe yield losses of wheat and barley [8,9], which are the second and fourth most produced grains in the world, respectively [10]. Moreover, barley is a model organism to study plant-pathogen interactions in cereals.…”

Section: Introductionmentioning

confidence: 99%

Identification of novel genetic factors underlying the host-pathogen interaction between barley (Hordeum vulgare L.) and powdery mildew (Blumeria graminis f. sp. hordei)

et al. 2020

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Powdery mildew is an important foliar disease of barley (Hordeum vulgare L.) caused by the biotrophic fungus Blumeria graminis f. sp. hordei (Bgh). The understanding of the resistance mechanism is essential for future resistance breeding. In particular, the identification of race-nonspecific resistance genes is important because of their regarded durability and broad-spectrum activity. We assessed the severity of powdery mildew infection on detached seedling leaves of 267 barley accessions using two poly-virulent isolates and performed a genome-wide association study exploiting 201 of these accessions. Two-hundred and fourteen markers, located on six barley chromosomes are associated with potential race-nonspecific Bgh resistance or susceptibility. Initial steps for the functional validation of four promising candidates were performed based on phenotype and transcription data. Specific candidate alleles were analyzed via transient gene silencing as well as transient overexpression. Microarray data of the four selected candidates indicate differential regulation of the transcription in response to Bgh infection. Based on our results, all four candidate genes seem to be involved in the responses to powdery mildew attack. In particular, the transient overexpression of specific alleles of two candidate genes, a potential arabinogalactan protein and the barley homolog of Arabidopsis thaliana's Light-Response Bric-a-Brac/-Tramtrack/-Broad Complex/-POxvirus and Zinc finger (AtLRB1) or AtLRB2, were top candidates of novel powdery mildew susceptibility genes.

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“…Blumeria graminis f. sp. hordei (Bgh) is a model obligate biotrophic fungus that causes powdery mildew on barley (Hordeum vulgare L.), leading to nearly 40% yield loss in cereals (Draz et al, 2019). When a Bgh spore lands on a barley leaf, it first lays down a primary germ tube that adheres it to the waxy cuticle .…”

Section: Overviewmentioning

confidence: 99%

“…hordei (Bgh), the causal agent of barley (Hordeum vulgare L.) powdery mildew, is an economically important biotrophic fungus (Murray and Brennan, 2010). The association between Bgh and barley represents a principle model for interactions among Triticeae grain crops and biotrophic pathogens, which are constrained to colonize and gain their nutrients from living tissue (Draz et al, 2019). During infection, Bgh releases avirulence (AVR) effector proteins into the host via haustorial feeding structures .…”

Section: Introductionmentioning

confidence: 99%

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Biological and molecular insights on MLA-based resistance of barley to powdery mildew derived from investigation of an in-frame Lys-Leu deletion in the essential protein SGT1

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Powdery mildew susceptibility of spring wheat cultivars as a major constraint on grain yield

Cited by 35 publications

References 31 publications

Genetic diversity, antifungal evaluation and molecular docking studies of Cu-chitosan nanoparticles as prospective stem rust inhibitor candidates among some Egyptian wheat genotypes

Genetic diversity, antifungal evaluation and molecular docking studies of Cu-chitosan nanoparticles as prospective stem rust inhibitor candidates among some Egyptian wheat genotypes

Identification of novel genetic factors underlying the host-pathogen interaction between barley (Hordeum vulgare L.) and powdery mildew (Blumeria graminis f. sp. hordei)

Biological and molecular insights on MLA-based resistance of barley to powdery mildew derived from investigation of an in-frame Lys-Leu deletion in the essential protein SGT1

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