Several ratios of silver/selenium (Ag/Se) nanoparticles generated via laser ablation were used to create nanocomposite scaffolds based on polyvinylidene difluoride (PVDF). XRD, FTIR, and HR-TEM were used to examine the nanocomposite membrane that were created. XRD, FTIR results confirm the interaction and complexation between PVDF and (Ag@Se) nanoparticles. In dark and light medium, the antibacterial activity of the produced compositions are evaluated. Additionally, the removal of methylene blue from liquids was detected under visible light. The inhibition zone in visible light of 3(Ag@Se) have the highest values, it was 38 ± 1.6, 41 ± 1.2 mm for Escherichia coli and Staphylococcus aureus, respectively. The removal efficiency rises from 88.8, 89.7, 93.6, to 98.3% upon the Ag@Se contributions after 240 min of continuous irradiation of visible light.