Power-line frequency electromagnetic fields do not induce changes in phosphorylation, localization, or expression of the 27-kilodalton heat shock protein in human keratinocytes.

Shi, Baojun; Farboud, Behnom; Nuccitelli, Richard; Isseroff, Roslyn Rivkah

doi:10.1289/ehp.5395

Cited by 27 publications

(24 citation statements)

References 46 publications

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“…Heat shock proteins (HSP) are used as markers for cell responses against various stresses (13). HSP serve protective functions such as apoptosis and act as molecular chaperones during protein biosynthesis and tissue repair (14).…”

Section: Discussionmentioning

confidence: 99%

“…We used scanning electron microscopy (SEM) and characterized the expression of HSP27 mRNA, which is present in osteoblasts lining newly-formed bone. HSP27 mRNA helps repair denatured proteins or promote their degradation after various types of stress (12)(13)(14). Moreover, expression of type I collagen and alkaline phosphatase (ALP) mRNAs was also evaluated using reverse transcription polymerase chain reaction (RT-PCR) (15)(16).…”

Section: Introductionmentioning

confidence: 99%

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Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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Abstract:The aim of this study was to examine the effect of centrifugal force on rat bone marrow osteoblast-like cells (RBM) in vitro. RBM were seeded and cultured for 2 days. Centrifugal forces were added to the cells in the culture dishes using a centrifuge for 20 min either at 600 rpm or 4,800 rpm. The cells without centrifugation were used as control. The expression of HSP27 mRNA of both experimental groups after addition of centrifugal force for 3 hrs was significantly up-regulated compared with the control group. In the 4,800 rpm groups, the cells showed contracted shapes compared to the controls and the 600 rpm groups after treatment with centrifugal force. The maximum height of each cell in the control group was 2.00 ± 0.33 μm. The maximum height of each cell in the 600 rpm group was 1.50 ± 0.23 μm, and in the 4,800 rpm group it was 1.13 ± 0.23 μm. The proliferation ratio in the 4,800 rpm group at day 3 was significantly higher than in the other group. The expression of type I collagen and alkaline phosphatase (ALP) mRNAs in both experimental groups increased at earlier time periods compared to the control groups. ALP activity revealed no significant difference between the experimental groups and the control group. A centrifugal force of 4,800 rpm might promote the proliferation and early differentiation of rat bone marrow cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

et al. 2009

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“…The expression of HSP16, HSP27 and HSP70 has been reported to be increased at magnetic-field strengths ranging from 8 Tt o 500 mT (15)(16)(17). However, other studies have reported that magnetic-field strengths of a similar range (6.3 T-50 mT) have no effect on heat-shock protein expression in a variety of biological models (18)(19)(20)(21). The physiological implications of field-induced heat-shock protein expression are unknown, but they could range from therapeutically beneficial to detrimental.…”

Section: Introductionmentioning

confidence: 99%

Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes

et al. 2004

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Although evidence is controversial, exposure to environmental power-frequency magnetic fields is of public concern. Cells respond to some abnormal physiological conditions by producing cytoprotective heat-shock (or stress) proteins. In this study, we determined whether exposure to power-frequency magnetic fields in the range 0-100 T rms either alone or concomitant with mild heating induced heat-shock protein gene expression in human leukocytes, and we compared this response to that induced by heat alone. Samples of human peripheral blood were simultaneously exposed to a range of magnetic-field amplitudes using a regimen that was designed to allow field effects to be distinguished from possible artifacts due to the position of the samples in the exposure system. Power-frequency magnetic-field exposure for 4 h at 37؇C had no detectable effect on expression of the genes encoding HSP27, HSP70A or HSP70B, as determined using reverse transcriptase-PCR, whereas 2 h at 42؇C elicited 10-, 5-and 12-fold increases, respectively, in the expression of these genes. Gene expression in cells exposed to power-frequency magnetic fields at 40؇C was not increased compared to cells incubated at 40؇C without field exposure. These findings and the extant literature suggest that power-frequency electromagnetic fields are not a universal stressor, in contrast to physical agents such as heat. ᭧

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“…The studies conducted on human keratinocytes subjected to 100 T showed that power line electromagnetic fields cannot induce changes in phosphorylation, localization or expression of the HSP 27 [43,44]. However, the cultures of human endothelial cell lines exposed to mobile phone radiation (RF-EMF) showed an increased expression and phosphorylation of HSP 27.…”

Section: Discussionmentioning

confidence: 99%

Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity

Rajendra¹,

Sujatha²,

Sashidhar³

et al. 2012

Biodiscovery

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The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (p<0.01) and 50 µT (p<0.05) for 16 h as compared to control group. There was a statistically significant decrease (p<0.01) in the specific activity of caspase 9 at 100 µT in cells exposed for 16 h. However, no enhancement of DNA damage was observed at 5, 50 and 100 µT as evidenced by comet assay. Comet assay also confirmed the decreased cell death of exposed cells (100 µT). Experimental data suggests decreased apoptosis at 100 µT (50 Hz), possibly by the suppression of caspase 9 activity leading to the enhanced cell viability. Effect of ELF-EMF on primary lymphocytesBioDiscovery | www.biodiscoveryjournal.co.uk

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Power-line frequency electromagnetic fields do not induce changes in phosphorylation, localization, or expression of the 27-kilodalton heat shock protein in human keratinocytes.

Cited by 27 publications

References 46 publications

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

Mechanical force promotes proliferation and early differentiation of bone marrow derived osteoblast-like cells in vitro

Effect of 50 Hz Electromagnetic Fields on the Induction of Heat-Shock Protein Gene Expression in Human Leukocytes

Viability of unstimulated lymphocytes exposed to extremely low frequency electromagnetic fields is dependent on intensity

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