Poxvirus sensitivity of a novel diploid sheep embryonic heart cell line

Rhazi, Halima; Tifrouin, Ikram; Mikou, Karima; Belayadi, Oumaima; Safini, Najete; Alhyane, Meryem; Tadlaoui, Khalid Omari; Lenk, Matthias; Elharrak, Mehdi

doi:10.1007/s00705-023-05855-x

Cited by 2 publications

References 35 publications

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Generation and application of immortalized sheep fetal fibroblast cell line

Du,

Zhang,

Cao

et al. 2024

BMC Vet Res

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Background Primary sheep fetal fibroblasts (SFFCs) have emerged as a valuable resource for investigating the molecular and pathogenic mechanisms of orf viruses (ORFV). However, their utilization is considerably restricted due to the exorbitant expenses associated with their isolation and culture, their abbreviated lifespan, and the laborious procedure. Results In our investigation, the primary SFFCs were obtained and immortalized by introducing a lentiviral recombinant plasmid containing the large T antigen from simian virus 40 (SV40). The expression of fibronectin and vimentin proteins, activity of SV40 large T antigen, cell proliferation assays, and analysis of programmed cell death revealed that the immortalized large T antigen SFFCs (TSFFCs) maintained the same physiological characteristics and biological functions as the primary SFFCs. Moreover, TSFFCs demonstrated robust resistance to apoptosis, extended lifespan, and enhanced proliferative activity compared to primary SFFCs. Notably, the primary SFFCs did not undergo in vitro transformation or exhibit any indications of malignancy in nude mice. Furthermore, the immortalized TSFFCs displayed live ORFV vaccine susceptibility. Conclusions Immortalized TSFFCs present valuable in vitro models for exploring the characteristics of ORFV using various techniques. This indicates their potential for secure utilization in future studies involving virus isolation, vaccine development, and drug screening.

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Generation and application of immortalized sheep fetal fibroblast cell line

Du,

Zhang,

Cao

et al. 2024

BMC Vet Res

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Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

Yao,

Lin,

Zhu

et al. 2024

Preprint

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Background Lumpy skin disease (LSD) is caused by the lumpy skin disease virus (LSDV) and has emerged as a significant threat to the cattle and water buffalo industries in China. Investigating the epidemiological characteristics of LSDV will be beneficial to the control and prevention of this disease. In this study, a Taqman-Probe-Based real-time PCR (qPCR) assay was developed for detecting LSDV nucleotides, 2495 tissue or blood samples were collected from Yunnan province and tested using the established qPCR assay. The GPCR and p32 genes of the LSDV-positive samples were amplified, sequenced, and analyzed. Additionally, an infectious LSDV strain was isolated from one positive sample, and its biological characteristics were explored. Results The established qPCR assay demonstrated high sensitivity with a limit of detection of 4.83 copies/µL. Out of the 2495 collected specimens, six (0.24%) samples were positive for LSDV nucleotides, and all positive samples belonged to the skin scab samples. Molecular and phylogenetic analysis based on the P32 and GPCR genes revealed that six LSDV strains were genetically similar to field LSDV strains from China, Russia, and Thailand. One LSDV strain was isolated and replicated well in PST cells, while which was not sensitive to the MDBK cells. Conclusions This study provides the first report on the epidemiological characteristics of LSDV in Yunnan province of China, which confirmed the prevalence of LSDV in Cattle in this province. These findings will contribute to the development of effective strategies for the prevention and control of LSDV.

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Poxvirus sensitivity of a novel diploid sheep embryonic heart cell line

Cited by 2 publications

References 35 publications

Generation and application of immortalized sheep fetal fibroblast cell line

Generation and application of immortalized sheep fetal fibroblast cell line

Epidemiology and genetic characteristics of the lumpy skin disease virus in Yunnan province, China from 2019 to 2023

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