The importance of regionalization of Polish agriculture is constantly increasing, mostly due to its environmental, organizational and economic differences between regions. The process of regionalization has become especially visible after Polish accession to the European Union. The study compared the changes in the utilization of production capacity for selected voivodships, located in different parts of Poland, i.e. Dolnoślaskie and Opolskie (A) and Małopolskie and Podkarpackie (B), in the years 2002–2004 and 2012–2014. The main data sources were Central Statistical Office of Poland publications and the results of an own study. The analysis showed significant differences in the production capacity of Polish agriculture and in the utilization of production potential in different regions. The differences between the analyzed groups of voivodships were mostly due to management and intensity differences of agricultural production, while habitat and natural conditions were of a minor importance. Agriculture of Dolnośląskie and Opolskie proved to be the most effective in achieving production and environmental objectives.