PP2A alleviates oxidized LDL-induced endothelial dysfunction by regulating LOX-1/ROS/MAPK axis

Xu, Kang; Liu, Xiwen; Yin, Di; Ren, Guanghao; Zhao, Yue

doi:10.1016/j.lfs.2020.117270

Cited by 6 publications

(6 citation statements)

References 32 publications

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“…In addition, Ox-LDL-induced HUVECs model is one of the commonly used models in the study of atherosclerosis. Our previous studies have shown that Ox-LDL-treatment promotes inflammation and dysfunction of HUVECs [ 15 ]. Similarly, the present research has reached a consistent conclusion.…”

Section: Discussionmentioning

confidence: 99%

“…The HUVECs were inoculated into 6-well plates for 24 h. Apoptosis experiments in this study were detected using the corresponding Annexin V-FITC apoptosis detection kits (Solarbio, Beijing, China) according to the instructions. We followed the methods of Xu et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…The fluorescence was analyzed using fluorescence microscope (BD Biosciences, CA) and fluorescence microplate reader. We followed the methods of Xu et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…The data were analyzed by the 2 – ΔΔ CT method with GAPDH as a normalizing gene. We followed the methods of Xu et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…Then, the enhanced laboratories (ECL) darkroom development, Bio-Rad Laboratories (California, USA) scan record, and anti-GAPDH as internal reference were used for analysis and comparison. We followed the methods of Xu et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

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VGLL4 Protects against Oxidized-LDL-Induced Endothelial Cell Dysfunction and Inflammation by Activating Hippo-YAP/TEAD1 Signaling Pathway

Zhao

Qiu

et al. 2020

Mediators of Inflammation

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Vestigial-like 4 (VGLL4) has been found to have multiple functions in tumor development; however, its role in cardiovascular disease is unknown. The aim of this study was to investigate the effect of VGLL4 on the dysfunction and inflammatory response of Ox-LDL-induced human umbilical vein endothelial cells (HUVECs) and its mechanism, so as to provide a new theoretical basis for the diagnosis and treatment of atherosclerosis. In the present study, the protective activity of VGLL4 inhibiting Ox-LDL-induced apoptosis, oxidative stress, inflammation, and injury as well as its molecular mechanisms was examined using human umbilical vein endothelial cells (HUVECs). The results showed that the expression of VGLL4 was decreased with the increase of Ox-LDL concentration in HUVECs. In addition, the functional study found that VGLL4 overexpression alleviated Ox-LDL-induced oxidative stress, inflammation, and dysfunction and inhibited apoptosis. Further research found that VGLL4 regulated Hippo-YAP/TEAD1 signaling pathway, and the Hippo-YAP/TEAD1 signaling pathway was involved in the protective mechanism of VGLL4 on HUVECs. In conclusion, it suggests that VGLL4 protects against oxidized-LDL-induced endothelial cell dysfunction by activating the Hippo-YAP/TEAD1 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The fluorescence was analyzed using fluorescence microscope (BD Biosciences, CA) and fluorescence microplate reader. We followed the methods of Xu et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…The data were analyzed by the 2 – ΔΔ CT method with GAPDH as a normalizing gene. We followed the methods of Xu et al [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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