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Campanella, James J.; Bakllamaja, Vinela; Restieri, Tracie; Vomacka, Michael; Herron, Jason; Patterson, Megan; Shahtaheri, Shahin

doi:10.1023/a:1022528318188

Cited by 15 publications

(4 citation statements)

References 17 publications

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“…The decrease in glycine content of mtpin4 may affect various vital metabolic processes and seed development. Plants use various mechanisms to regulate IAA levels, such as conjugation with amino acids and hydrolysis, which can affect IAA quantity and activity (Campanella et al., 2003; Ljung et al., 2002; Normanly, 1997; Rampey et al., 2004). IAA can be converted into its stored form by conjugation with glycine (Bajguz & Piotrowska, 2009; Rampey et al., 2004).…”

Section: Discussionmentioning

confidence: 99%

MtPIN4 plays critical roles in amino acid biosynthesis and metabolism of seed in Medicago truncatula

Jiang,

Xie,

et al. 2024

The Plant Journal

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SUMMARYThe regulation of seed development is critical for determining crop yield. Auxins are vital phytohormones that play roles in various aspects of plant growth and development. However, its role in amino acid biosynthesis and metabolism in seeds is not fully understood. In this study, we identified a mutant with small seeds through forward genetic screening in Medicago truncatula. The mutated gene encodes MtPIN4, an ortholog of PIN1. Using molecular approaches and integrative omics analyses, we discovered that auxin and amino acid content significantly decreased in mtpin4 seeds, highlighting the role of MtPIN4‐mediated auxin distribution in amino acid biosynthesis and metabolism. Furthermore, genetic analysis revealed that the three orthologs of PIN1 have specific and overlapping functions in various developmental processes in M. truncatula. Our findings emphasize the significance of MtPIN4 in seed development and offer insights into the molecular mechanisms governing the regulation of seed size in crops. This knowledge could be applied to enhance crop quality by targeted manipulation of seed protein regulatory pathways.

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Section: Discussionmentioning

confidence: 99%

MtPIN4 plays critical roles in amino acid biosynthesis and metabolism of seed in Medicago truncatula

Jiang,

Xie,

et al. 2024

The Plant Journal

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“…Indoleacetamide hydrolase catalyzes the formation of indole acetic acid from indole acetamide, and leads directly to the increase of auxin level in plant tissues (Staswick et al, 2005). TaIAR3, an ortholog for the Arabidopsis IAR3 auxin amidohydrolase gene isolated from wheat is able to cleave conjugates of auxin molecules with longer side chains (LeClere et al, 2002;Campanella et al, 2003). In addition, the TaIAR3 mRNA shows a different spatial and temporal regulation compared to IAR3 from Arabidopsis (Davies et al, 1999;Rampey et al, 2003), suggesting that TaIAR3 enzymes may be employed in different developmental pathways in wheat.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Dynamic Changes of Endogenous Plant Hormones and Hormone-Related Gene Expression between Near Isogenic Lines with Normal Spike and Branched Spike Wheat

Li¹,

Jiang²,

He³

et al. 2015

JAS

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Branched spike wheat is a hexaploid wheat with branched rachis on its main rachis, and branched rachises in the shoot apex occur and develop just after the two ridges stage. In this study, dynamic changes of four endogenous plant hormones [indole-3-acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and zeatin riboside (ZR)] and the expression of a hormone-related gene were measured among wheat lines with different genotypes and spike morphologies. It is a basis for further exploring the developmental mechanisms of branched spike and for breeding new wheat lines with more spikelets and grains. A group of near isogenic lines (NILs), including one branched spike line, and three normal spike type lines was sampled at four time points from the end of two ridges stage to the floret differentiation stage. The endogenous hormones in young shoots, young leaves and tillering nodes in these lines were measured and the expressions of TaIAR3, encoding IAA amide hydrolase which involvs in the IAA synthesis was detected. The results showed that the contents of IAA exhibited a significant different pattern in branched spike line from those in normal spike lines, while the contents of GA, ABA and ZR showed similar change patterns. The expressions of TaIAR3 were also significantly different between branched spike line and normal spike lines. Both the IAA content and TaIAR3 expression in branched spike wheat were much higher than those in the normal spike lines. These results suggest that quantitative changes in auxin may correspond to different spike morphology. Higher IAA content and TaIAR3 expression might be benefical for promoting the formation and elongation of branched rachis in branched spike wheat line.

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“…[39] Interestingly, small changes in the amino acid sequence resulted in a different set of auxin conjugates, which were hydrolyzed by the enzyme. [18] (ILR1 preferentially cleaves IAA-Phe and IAA-Leu; ILL1, ILL2 and IAR3 prefer IAA-Ala; sILR1 is more specific for IAA-Gly and IAA-Ala).…”

Section: Auxin Amidohydrolases: Discoverymentioning

confidence: 99%

Auxin Amidohydrolases – From Structure to Function: Revisited

Smolko¹,

Ludwig‐Müller²,

Salopek‐Sondi³

2018

Croat. Chem. Acta

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The control of plant growth and development is a well-coordinated process between exogenous and endogenous signals. Auxins are plant hormones belonging to the endogenous signals, which control a vast array of different processes. While auxins are growth promoting at low concentrations, higher levels are often inhibitory. Therefore, the tight control of auxin concentrations in a given plant tissue is essential. Among several processes that participate in auxin homeostasis, we focused herein on the process of reversible auxin conjugation that considers the synthesis of inactive auxin conjugates, which can be hydrolyzed back to the active form by so called auxin conjugate hydrolases. Although these proteins have been known for quite some time, their role in plants is still not clear, especially since novel hydrolases with different substrate specificities have been isolated. Thus, we have revisited the knowledge about auxin hydrolases, from their structure and biochemistry to the role in plant development and in dealing with unfavorable climate conditions.

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Cited by 15 publications

References 17 publications

MtPIN4 plays critical roles in amino acid biosynthesis and metabolism of seed in Medicago truncatula

MtPIN4 plays critical roles in amino acid biosynthesis and metabolism of seed in Medicago truncatula

Comparison of Dynamic Changes of Endogenous Plant Hormones and Hormone-Related Gene Expression between Near Isogenic Lines with Normal Spike and Branched Spike Wheat

Auxin Amidohydrolases – From Structure to Function: Revisited

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