PPARγ activity in subcutaneous abdominal fat tissue and fat mass gain during short-term overfeeding

Abstract

Objective: As the peroxisome proliferator-activated receptor g (PPARg) plays a central role in fat mass regulation, we investigated whether initial subcutaneous PPARg activity is related to fat mass generation during overfeeding. Subjects: Fourteen healthy female subjects (age 2574 years, BMI 22.172.3 kg/m 2 ). Design and measurements: Subjects were overfed with a diet supplying 50% more energy than baseline energy requirements for 14 days. Fasting blood samples were analyzed for leptin, insulin and glucose. Fasting subcutaneous abdominal fat biopsies were obtained for analysis of PPARg1, PPARg2, aP2 and UCP2 mRNAs. Results: Initial PPARg1 and 2, aP2 and UCP2 mRNAs were not related to fat gain (P40.12). However, PPARg1, PPARg2 and aP2 mRNA changes were positively related to changes in plasma leptin (Po0.05) and, except aP2 (P ¼ 0.06), to fat gain (Po0.05). PPARg and aP2 mRNA changes were positively related (Po0.01), indicating that PPARg mRNA levels reflected PPARg activity. Conclusion: These data suggest that the ability to increase PPARg activity might be involved in the susceptibility to gain weight during a positive energy balance.

X‐box binding protein 1 is a novel key regulator of peroxisome proliferator‐activated receptor γ2

“…The Ppar γ 2 mRNA level of adipose tissue increased in obesity . Given our results showing the XBP1s‐mediated positive regulation of PPARγ2, we investigated the possible correlation of Xbp1s with BMI.…”

Cell death–inducing DFF45-like effector C is reduced by caloric restriction and regulates adipocyte lipid metabolism

“…Importantly, PPARγ mRNA levels have been shown to correlate to transcriptional activity in human adipose tissue and to be down-regulated after weight loss [20,21]. Thus, decreased PPARγ activity may contribute to the observed down-regulation of CIDEC in response to caloric restriction.…”

Dietary fat oxidation as a function of body fat

“…Peroxisome proliferator-activated receptor-gamma (PPARg) regulates the expression of genes involved in fat storage [15]. An increase in PPARg expression during overfeeding was positively correlated to fat mass gain [16]. Thus, the ability to increase PPARg activity might be involved in the susceptibility to gain weight during a positive energy balance.…”