PpCBFs selectively regulate PpDAMs and contribute to the pear bud endodormancy process

Li, Jianzhao; Yan, Xue; Yang, Qinsong; Ma, Yihan; Yang, Bo; Tian, Jun; Teng, Yuanwen; Bai, Songling

doi:10.1007/s11103-019-00837-7

Cited by 36 publications

(38 citation statements)

References 39 publications

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“…In a previous study, we observed that PpyCBF4 expression is induced by ABA and activates the PpyDAM3 promoter (Ahmad et al, ; Li et al, ). Because we also identified ABREs in the PpyCBF4 promoter region (Figure S6b), we examined whether PpyABF3 is expressed downstream of the ABA pathway and upstream of PpyCBF4 expression.…”

Section: Resultsmentioning

confidence: 87%

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

Yang

et al. 2020

Plant Cell & Environment

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101

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Bud dormancy is indispensable for the survival of perennial plants in cold winters.Abscisic acid (ABA) has essential functions influencing the endo-dormancy status. Dormancy-associated MADS-box/SHORT VEGETATIVE PHASE-like genes function downstream of the ABA signalling pathway to regulate bud dormancy. However, the regulation of DAM/SVP expression remains largely uncharacterized. In this study, we confirmed that endo-dormancy maintenance and PpyDAM3 expression are controlled by the ABA content in pear (Pyrus pyrifolia) buds. The expression of pear ABRE-BINDING FACTOR3 (PpyABF3) was positively correlated with PpyDAM3 expression. Furthermore, PpyABF3 directly bound to the second ABRE in the PpyDAM3 promoter to activate its expression. Interestingly, both PpyABF3 and PpyDAM3 repressed the cell division and growth of transgenic pear calli. Another ABA-induced ABF protein, PpyABF2, physically interacted with PpyABF3 and disrupted the activation of the PpyDAM3 promoter by PpyABF3, indicating DAM expression was precisely controlled. Additionally, our results suggested that the differences in the PpyDAM3 promoter in two pear cultivars might be responsible for the diversity in the chilling requirements. In summary, our data clarify the finely tuned regulatory mechanism underlying the effect of ABA on DAM gene expression and provide new insights into ABA-related bud dormancy regulation. K E Y W O R D S ABF3, Abscisic acid, bud dormancy, DAM, pear

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Section: Resultsmentioning

confidence: 87%

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

Yang

et al. 2020

Plant Cell & Environment

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101

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“…PtCBF4 was detectable only in 'Walker' leaves, while PtCBF1 and PtCBF6 were the most intensively expressed genes in 'Okanese'. Whether they have different functions, as was suggested for PpCBF4 [37] in pear, is still unclear. It is also remarkable that PtCBF5 was the only gene which was not induced during the CBF-burst on the 10 th day in 'Okanese' buds but was the most intensively up-regulated in 'Walker' buds on the 1 st day.…”

Section: Expression Pattern Of Cbf Genesmentioning

confidence: 96%

“…Additionally, low temperature up-regulated 8 CBFs in Prunus mume which subsequently induced all six DAM genes resulting in dormancy development [36]. Under natural dormancy induction conditions, 3 out of 4 CBFs showed similar expression trends in Pyrus pyrifolia bud tissues, while PpCBF1 showed a different induction kinetic [37]. During an arti cial chilling test, PpCBF1 was the only CBF highly expressed, while PpCBF2 was repressed intensively, and the levels of PpCBF3 and PpCBF4 were undetectable.…”

Section: Expression Pattern Of Cbf Genesmentioning

confidence: 99%

“…While the main regulator of growth cessation and dormancy induction in woody species is short photoperiod, it may be moderated by, and interact with temperature [17]. The increasing con rmation of direct regulation by cold-induced CBFs on DAM gene expression [34,37,55], Niu et al (2016) provided evidence and proposed a model in which CBF induces DAM and DAM downregulates FT which then suppresses growth and stimulates the development of dormancy [38]. Liu and Sherif (2019) further outlined a model integrating multiple phytohormonal networks regulated by DAM [25].…”

Section: The Relation Between Ptcbfs and Ptdam1 Expression Hormone Lmentioning

confidence: 99%

“…Japanese pear PpCBFs were able to induce the expression of PpDAM1-1 and PpMADS13-3 genes in transient reporter assays [55]. Different biochemical methods revealed that pear PpCBF2 and PpCBF4 genes are able to bind to the promoter of PpDAM1 gene, activating its expression, and revealed that PpCBF1, PpCBF2, PpCBF3, PpCBF4 genes can activate PpDAM3 gene [37]. These results not just demonstrate the CBF -DAM signalling pathway is involved in endodormancy development, but also demonstrate a certain level of CBF functional redundancy.…”

Section: Introductionmentioning

confidence: 99%

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Elucidation of Molecular and Hormonal Background of Early Growth Cessation and Endodormancy Induction in Two Contrasting Populus Hybrid Cultivars

Boldizsár

Soltész

Tanino

et al. 2020

Preprint

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Background Over the life cycle of perennial trees, the dormant state enables the avoidance of abiotic stress conditions. The growth cycle can be partitioned into induction, maintenance and release and is controlled by complex interactions between many endogenous and environmental factors. While phytohormones have long been linked with dormancy, there is increasing evidence of regulation by DAM and CBF genes. To reveal whether the expression kinetics of CBFs and their target PtDAM1 is related to growth cessation and endodormancy induction in Populus, two hybrid poplar cultivars were studied.Results Growth cessation, dormancy status and expression of six PtCBFs and PtDAM1 were analyzed. The ‘Okanese’ hybrid cultivar ceased growth rapidly, was able to reach endodormancy, and exhibited a significant increase of several PtCBF transcripts in the buds on the 10th day. The ‘Walker’ cultivar had delayed growth cessation, was unable to enter endodormancy, and showed much lower CBF expression in buds. Expression of PtDAM1 peaked on the 10th day only in the buds of ‘Okanese’. Besides, in the leaves of the two cultivars, PtDAM1 was not expressed while the CBFs expression pattern was reversed. Leaf phytohormones in both cultivars followed similar profiles during growth cessation but differentiated based on cytokinins which were largely reduced, while the Ox-IAA and iP7G increased in ‘Okanese’ compared to ‘Walker’. Surprisingly, ABA concentration was reduced in leaves of both cultivars. However, the metabolic deactivation product of ABA, phaseic acid, exhibited an early peak on the first day in ‘Okanese’.Conclusions Our results indicate that PtCBFs and PtDAM1 have differential kinetics and spatial localization which may be related to early growth cessation and endodormancy induction under the regime of low night temperature and short photoperiod in poplar. Unlike buds, PtCBFs and PtDAM1 expression levels in leaves were not associated with early growth cessation and dormancy induction under these conditions. Our study provides new evidence that the degradation of auxin and cytokinins in leaves may be an important regulatory point in a CBF-DAM induced endodormancy. Further investigation of other PtDAMs in bud tissue and a study of both growth-inhibiting and the degradation of growth-promoting phytohormones is warranted.

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VvDAM-SVPs genes are regulated by FLOWERING LOCUS T (VvFT) and not by ABA/low temperature-induced VvCBFs transcription factors in grapevine buds

Vergara

Noriega

Pérez

2021

Planta

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PpCBFs selectively regulate PpDAMs and contribute to the pear bud endodormancy process

Cited by 36 publications

References 39 publications

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

ABA‐responsive ABRE‐BINDING FACTOR3 activatesDAM3expression to promote bud dormancy in Asian pear

Elucidation of Molecular and Hormonal Background of Early Growth Cessation and Endodormancy Induction in Two Contrasting Populus Hybrid Cultivars

VvDAM-SVPs genes are regulated by FLOWERING LOCUS T (VvFT) and not by ABA/low temperature-induced VvCBFs transcription factors in grapevine buds

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