PpMYB52 negatively regulates peach bud break through the gibberellin pathway and through interactions with PpMIEL1

Abstract

Bud dormancy, which enables damage from cold temperatures to be avoided during winter and early spring, is an important adaptive mechanism of deciduous fruit trees to cope with seasonal environmental changes and temperate climates. Understanding the regulatory mechanism of bud break in fruit trees is highly important for the artificial control of bud break and the prevention of spring frost damage. However, the molecular mechanism underlying the involvement of MYB TFs during the bud break of peach is still unclear. In this study, we isolated and identified the PpMYB52 (Prupe.5G240000.1) gene from peach; this gene is downregulated in the process of bud break, upregulated in response to ABA and downregulated in response to GA. Overexpression of PpMYB52 suppresses the germination of transgenic tomato seeds. In addition, Y2H, Bimolecular fluorescence complementation (BiFC) assays verified that PpMYB52 interacts with a RING-type E3 ubiquitin ligase, PpMIEL1, which is upregulated during bud break may positively regulate peach bud break by ubiquitination-mediated degradation of PpMYB52. Our findings are the first to characterize the molecular mechanisms underlying the involvement of MYB TFs in peach bud break, increasing awareness of dormancy-related molecules to avoid bud damage in perennial deciduous fruit trees.

Climate change impacts on temperate fruit and nut production: a systematic review

“…Novel genomic technologies developed in recent years have provided new gene mapping techniques and allowed the development of molecular markers that can accelerate the efficient deployment of beneficial alleles to new cultivars ( Lobos and Hancock, 2015 ; Iwata et al., 2016 ; Whitaker et al., 2020 ). For instance, because chilling requirements are becoming a limiting factor for cultivar adaptation in warming environments, scientists are working on identifying genes associated with bud dormancy and flowering time in temperate crops to elucidate the genetic control of these processes and develop new cultivars adapted to different climatic conditions ( Castede et al., 2014 ; Romeu et al., 2014 ; Allard et al., 2016 ; Prudencio et al., 2018 ; Cantin et al., 2020 ; Prudencio et al., 2021 ; Zhang et al., 2022 ). New technologies have also enabled the identification of the genetic regions controlling traits such as flowering time ( Ruiz et al., 2019 ; Branchereau et al., 2023 ) and bud dormancy ( Allard et al., 2016 ; Prudencio et al., 2018 , Prudencio et al., 2021 ), as well as traits related to temperature requirements for endodormancy release ( Romeu et al., 2014 ).…”