Pra-pemuliaan  Aneka Kacang dalam Mendukung Proses Pemuliaan  untuk Perakitan Varietas Unggul Baru

Asadi, Asadi; Lestari, Puji; Dewi, Nurwita

doi:10.21082/jbio.v12n1.2016.p51-62

Cited by 4 publications

(4 citation statements)

References 30 publications

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“…Characterization of germplasm collections is needed in pre-breeding activities [9]. The purpose of characterization is to identify accessions that have superior character so that they can be used as parents in the breeding program.…”

Section: Introductionmentioning

confidence: 99%

Cowpea [Vigna unguiculata (L.) Walp.] Yield Variance and Supported Character

Putri¹,

Nugrahaeni²

2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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Cowpea is an underutilized legume that has been cultivating in Indonesia. But, the availability of cowpea variety is limited. Cowpea breeding program is started with gene source characterization of germplasm. This research aims to study the yield and its supported component variability of cowpea germplasm. One hundred and fifty cowpea accessions were planted using randomized block design with two replications. Observation was done for days of flowering, days of maturity, plant height, number of branches, number of pods per plant, pod length, number of seeds per pod, seed weights per plant, 100 seeds weight, and seed weights per plot. Statistic descriptive, analysis of variance, and was analyzed with MSTAT 1.4 version software, while path analysis was done with SPSS 19 version. Days of flowering was ranged 32-53 das, days of maturity 60-79 das, plant height 16-51.3 cm, number of branches 0-5, number of pods per plant 13-93, pod length 9.1-22.8 cm, number of seeds per pod 12-20, seed weight 7.47-76.17 g per plant, 100 seeds weight 4.19-18.5 g, and seed weight per plot 45.61-346.58 g. There were significant differences between the genotype in leaf length x width, plant height, pod length, number of pods per plant, weight of 100 seeds, and seed weight per plot. All of the observed characters were correlated highly significant to yield, except days of maturity, leaf length x width, and pod length which correlated significantly, also number of branches and number of seeds per pod which not correlated significantly to yield. Selection was held for yield character using 20% selection index and there were 19 accessions selected, with 269.58-326.48 g seed yield per plot.

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Section: Introductionmentioning

confidence: 99%

Cowpea [Vigna unguiculata (L.) Walp.] Yield Variance and Supported Character

Putri¹,

Nugrahaeni²

2021

Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)

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“…The use of Marker Assisted Selection (MAS) in selection is more profitable compared to conventional selection. With the help of molecular markers, selection is carried out on plant genetic characteristics, which are not influenced by environmental factors, making the selection process faster, more precise, and relatively more cost and time-effective [8] [9]. The selection process, with the help of molecular markers, requires information on markers associated with the desired trait, so there is a need for a validation/testing process for the ability of these markers to identify related traits.…”

Section: Introductionmentioning

confidence: 99%

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

Suprayogi,

Oktaviani,

Riyanto

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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The development of black rice with a fluffier texture at Universitas Jenderal Soedirman, which began in 2004, was carried out by crossing local black rice with the Mentik Wangi variety. At present, six black rice advanced breeding lines are ready for multi-location yield trials. Meanwhile, developing molecular marker-based plant breeding methods can facilitate a more efficient selection process. An important stage in developing molecular markers is marker validation, which aims to test the effectiveness in determining phenotypes in various genotypic and in new populations that differ from others based on the link between the marker and the expected character identified. This research aimed to determine molecular markers that can differentiate the level of amylose content in populations of F8 and F9 lines. The research was carried out on 10 (ten) genotypes consisting of the control variety Logawa, IRPM 112-19- 56, black rice, Mentik Wangi, and six lines. The amplification of the RM190, Wx and SSIIa markers showed polymorphic band. Based on the simple linear regression analysis, in F9, the SSIIa marker had the highest coefficient of determination compared to other markers. Hence, it had the greatest possibility of being a marker for selecting rice based on amylose content.

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“…Therefore, populations obtained through crossbreeding need more screening for resistance-related traits inherited from the parental genotypes, and the emerging genetic diversity must require analysis using molecular markers to identify genes responsible for the resistance. Detecting parental strains with dominant or recessive alleles and individuals with genetic variations can proceed using molecular markers (Azrai, 2005). Specifically, Inter-simple sequence repeat (ISSR) markers based on PCR can safely help amplify two short nucleotides.…”

Section: Introductionmentioning

confidence: 99%

Shallot (Allium Cepa Var. Aggregatum) Genotypes and Their Crossbreds Resistance to Fusarium Wilt Disease

MAHARIJAYA

2024

SABRAOJBG

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Shallots are valuable horticultural products consumed worldwide that offer numerous health benefits. Fusarium wilt disease severely limits shallot production, reducing bulb yield by up to 50%; furthermore, wilt disease-resistant shallot cultivars are insufficient. This study confirmed that the 'Batu Ijo' cultivar is resistant to Fusarium wilt, with the lowest percentage of affected bulbs (29.5%). In contrast, the cultivar 'Bima Brebes' showed the highest incidence of Fusarium infection (41.6%). Neither 'Batu Ijo' nor 'Katumi' became resistant parents when crossbred with susceptible plants because both failed to bloom until the end of the plant generative stage. Thus, to generate diverse segregating populations, a cross combination derived from 'Biru Lancor' (resistant parent) and 'Bima Brebes' ensued. The results showed lower values for the number of mature capsules, true seed shallot weight per plant, and seedling vigor were evident in the progenies than in the parental genotypes. These poor crossing results were due to several factors, including an inappropriate photoperiod for the crossbreeding of 'Bima Brebes' and 'Biru Lancor.' Out of 30, 17 plants showed symptoms of Fusarium wilt disease. Meanwhile, the 13 resistant individuals exhibited similarities with the male parent 'Biru Lancor' during ISSR molecular analysis, suggesting that the male parent may play a role in transmitting disease resistance. The selection of parental individuals and crossbreeding factors are crucial for successful shallot crossbreeding. This study bears significant implications for further development of shallot crossbreeding, potentially leading to new disease-resistant cultivars.

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Pra-pemuliaan Aneka Kacang dalam Mendukung Proses Pemuliaan untuk Perakitan Varietas Unggul Baru

Cited by 4 publications

References 30 publications

Cowpea [Vigna unguiculata (L.) Walp.] Yield Variance and Supported Character

Cowpea [Vigna unguiculata (L.) Walp.] Yield Variance and Supported Character

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

Shallot (Allium Cepa Var. Aggregatum) Genotypes and Their Crossbreds Resistance to Fusarium Wilt Disease

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