Practical application of Elisa for detection of vertical transmission of leukosis virus in commercial layer hens

Ignjatovic, Jagoda; Bagust, T. J.

doi:10.1080/03079458308436196

Cited by 16 publications

(3 citation statements)

References 9 publications

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“…Ignjatovic andBagust, 1983), the discussion is further aimed at ALV-p27 detection in albumen samples. The increase of binding characteristics by MCAs prompted us to modify slightly the DAS-ELISA procedures.…”

Section: Gjf De Boer and Ajxme Osterhausmentioning

confidence: 99%

Application of monoclonal antibodies in the avian leukosis virus GS‐antigen ELISA

Boer

Osterhaus

1985

Avian Pathology

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Five hybridoma cell lines which secrete antibodies to avian leukosis/ sarcoma (ALV) group-specific (gs) antigens (gag gene products) have been established. The hybrid cells resulted from fusion of P3/X63-Ag8.653 myeloma cells with splenocytes of BALB/c mice which had been immunised with purified avian myeloblastosis virus (AMV). Screening of supernatant fluids was performed by an indirect double antibody sandwich enzyme-linked immunosorbent assay (IDAS-ELISA) and the immunoelectroblotting technique. Three hybrid clones secrete monoclonal antibodies (MCA) to 27,000 dalton polypeptides (p27) and two hybridomas produce monoclonal antibody directed to 19,000 dalton phosphoprotein (pp19). All five monoclonal antibodies belong to mouse immunoglobulin isotype IgG(1). MCAs directed to different ALV-p27 epitopes can replace polyclonal rabbit or hamster anti-gs sera in the DAS-ELISA in use for the detection of congenitally ALV-shedding hens. In albumen samples a 16- to 32-fold increase of sensitivity of ALV gs-antigen detection was obtained as compared to the DAS-ELISA employing polyclonal sera. Gs-antigens of a purified AMV preparation were detectable up to minimal concentrations of 13 pg/ml.

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Section: Gjf De Boer and Ajxme Osterhausmentioning

confidence: 99%

Application of monoclonal antibodies in the avian leukosis virus GS‐antigen ELISA

Boer

Osterhaus

1985

Avian Pathology

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“…(We acknowledge the development of a computer program in BASIC by Dr R.H. Meloen and Mr J. Boerke). Since the prevalence of ALV-gsa in egg albumen samples is closely associated with congenital transmission of ALV (De Boer et al, 1983;Ignjatovic and Bagust, 1983), albumens are mainly employed for the detection of ALV shedding hens in eradication programmes (Spencer et al, 1976). Until now the detection of ALV shedding hens in basic breeding flocks in The Netherlands was routinely performed by direct complement fixation tests (CFT) (De Boer et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Application of monoclonal antibodies in the dutch avian leukosis control programme

Boer

Osterhaus²,

Kouwenhoven³

1985

Avian Pathology

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“…Subsequent interest in the eradication of LLV from commercial chicken flocks has in practice been replaced by reduction programmes since it was found that no single (and commercially feasible) method could identify all dams transmitting LLV Payne et al, 1982;Ignjatovic and Bagust, 1983). At present reduction programmes are carried out by the testing of flocks for the group-specific (gs)-antigen of LLV in albumen, usually by an enzymelinked immunosorbent assay (ELISA) (Smith et al, 1979;Ignjatovic and Bagust, 1982).…”

Section: Introductionmentioning

confidence: 99%

Effect of lymphoid Leukosis virus on performance of layer hens and the identification of infected chickens by tests on Meconia

Ignjatovic

Fraser²,

Bagust³

1986

Avian Pathology

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SUMMARYThe effect of subclinical infection with lymphoid leukosis virus (LLV) on the productivity traits of layer hens was investigated. In hens that shed gs-antigen of LLV to albumen, onset of sexual maturity was delayed by a mean of 11 days and the number of eggs laid was reducedby 68 per hen up to 75 weeks of age. Shedding hens laid on average 2 g lighter eggs and of lesser specific gravity. Thirty-four % less chickens were obtained in the reproduction programmes from LLV-shedders in comparison with non-shedders. LLV had no significant effect on fertility and hatchability. Reduced egg-related performance was only directly related to LLV-shedding and dams' shedding status had no effect on the egg-related performance of their LLV-free progeny. Dams' shedding status, however, correlated with higher mortality (10% higher) among their progeny. The percentage of non-layers was also higher in progeny of LLV-shedders. Meconia were highly suitable samples for identifying both transmitting dams and infected chickens but only if the test for infectious virus was performed. ELISA on meconia was less reliable than the test for virus and therefore is not recommended for the detection of residual of infected chickens in the flocks selected for reduced gs-antigen shedding.

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Practical application of Elisa for detection of vertical transmission of leukosis virus in commercial layer hens

Cited by 16 publications

References 9 publications

Application of monoclonal antibodies in the avian leukosis virus GS‐antigen ELISA

Application of monoclonal antibodies in the avian leukosis virus GS‐antigen ELISA

Application of monoclonal antibodies in the dutch avian leukosis control programme

Effect of lymphoid Leukosis virus on performance of layer hens and the identification of infected chickens by tests on Meconia

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