Practical application of PMA–qPCR assay for determination of viable cells of inter-species biofilm of Candida albicans–Staphylococcus aureus
Samuel Kendra,
Jarmila Czucz Varga,
Barbora Gaálová-Radochová
et al.
Abstract:Determining the number of viable cells by calculating colony-forming units is time-consuming. The evaluation of mixed biofilms consisting of different species is particularly problematic. Therefore, the aim of this study was to optimize a molecular method—propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) - for accurate and consistent differentiation between living and dead cells. In the practical experimental example, the number of genome copies representing living cells was determined in a… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.