Practical Aspects and Case Studies of Industrial Scale Fermentation

Magdouli, Sara; Saffar, Thana; Guedri, Tayssir; Rouissi, Tarek; Brar, Satinder Kaur; Blais, Jean-François

doi:10.1002/9781119248002.ch12

Cited by 4 publications

(2 citation statements)

References 123 publications

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“…The cellular utilization of glucose is retarded by the decreased expression of certain glycolytic enzymes [32]. A defect in membrane fluidity handicaps the solute transport systems of cells [33]. In contrast, a high level of crude glycerol induces the inhibitory effect on E. coli and the underlying mechanism may vary with the various compositions of crude glycerol.…”

Section: Resultsmentioning

confidence: 99%

A simple strategy to effectively produce d-lactate in crude glycerol-utilizing Escherichia coli

Yao-de

Liao

Chiang

et al. 2019

Biotechnol Biofuels

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BackgroundFed-batch fermentation has been conventionally implemented for the production of lactic acid with a high titer and high productivity. However, its operation needs a complicated control which increases the production cost.ResultsThis issue was addressed by simplifying the production scheme. Escherichia coli was manipulated for its glycerol dissimilation and d-lactate synthesis pathways and then subjected to adaptive evolution under high crude glycerol. Batch fermentation in the two-stage mode was performed by controlling the dissolved oxygen (DO), and the evolved strain deprived of poxB enabled production of 100 g/L d-lactate with productivity of 1.85 g/L/h. To increase productivity, the producer strain was further evolved to improve its growth rate on crude glycerol. The fermentation was performed to undergo the aerobic growth with low substrate, followed by the anaerobic production with high substrate. Moreover, the intracellular redox of the strain was balanced by fulfillment of the anaerobic respiratory chain with nitrate reduction. Without controlling the DO, the microbial fermentation resulted in the homofermentative production of d-lactate (ca. 0.97 g/g) with a titer of 115 g/L and productivity of 3.29 g/L/h.ConclusionsThe proposed fermentation strategy achieves the highest yield based on crude glycerol and a comparable titer and productivity as compared to the approach by fed-batch fermentation. It holds a promise to sustain the continued development of the crude glycerol-based biorefinery.

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Section: Resultsmentioning

confidence: 99%

A simple strategy to effectively produce d-lactate in crude glycerol-utilizing Escherichia coli

Yao-de

Liao

Chiang

et al. 2019

Biotechnol Biofuels

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“…In the present study, upscale in a 3 L working volume bioreactor led to a decrease in biomass and SCP production, while the protein content of dry biomass ranged between 34.5-49.6% (w/w). Those results could be explained by the fact that the scale-up of fermentation processes is a multidisciplinary approach and can be affected by multiple factors, including agitation, mass transfer of oxygen, shear damage and others [40], resulting in the altered physiology of microorganisms with constant metabolic shifts, leading to reduced growth and productivity. Yadav et al [17] evaluated the SCP production by K. marxianus (in batch and continuous mode trials) and simultaneous COD removal using cheese whey under 40.0 °C (pH = 3.5, ~50.0 g/L lactose) in a 10 L bioreactor.…”

Section: Discussionmentioning

confidence: 99%

Single-Cell Protein and Ethanol Production of a Newly Isolated Kluyveromyces marxianus Strain through Cheese Whey Valorization

Koukoumaki,

Papanikolaou,

Ioannou

et al. 2024

Foods

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The present work examined the production of single-cell protein (SCP) by a newly isolated strain of Kluyveromyces marxianus EXF-5288 under increased lactose concentration of deproteinized cheese whey (DCW) and different temperatures (in °C: 20.0, 25.0, 30.0 and 35.0). To the best of the authors’ knowledge, this is the first report examining the ability of Kluyveromyces marxianus species to produce SCP at T = 20.0 °C. Different culture temperatures led to significant differences in the strain’s growth, while maximum biomass and SCP production (14.24 ± 0.70 and 6.14 ± 0.66 g/L, respectively) were observed in the cultivation of K. marxianus strain EXF-5288 in shake-flask cultures at T = 20.0 °C. Increased DCW lactose concentrations (35.0–100.0 g/L) led to increased ethanol production (Ethmax = 35.5 ± 0.2 g/L), suggesting that K. marxianus strain EXF-5288 is “Crabtree-positive”. Batch-bioreactor trials shifted the strain’s metabolism to alcoholic fermentation, favoring ethanol production. Surprisingly, K. marxianus strain EXF-5288 was able to catabolize the produced ethanol under limited carbon presence in the medium. The dominant amino acids in SCP were glutamate (15.5 mg/g), aspartic acid (12.0 mg/g) and valine (9.5 mg/g), representing a balanced nutritional profile

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Novel β-glucanases along with xylanase identified in Thermomyces lanuginosus secretome for enhanced saccharification of different lignocellulosics

Brar

Raheja

Falco

et al. 2021

Biomass Conv. Bioref.

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Practical Aspects and Case Studies of Industrial Scale Fermentation

Cited by 4 publications

References 123 publications

A simple strategy to effectively produce d-lactate in crude glycerol-utilizing Escherichia coli

A simple strategy to effectively produce d-lactate in crude glycerol-utilizing Escherichia coli

Single-Cell Protein and Ethanol Production of a Newly Isolated Kluyveromyces marxianus Strain through Cheese Whey Valorization

Novel β-glucanases along with xylanase identified in Thermomyces lanuginosus secretome for enhanced saccharification of different lignocellulosics

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